Targeting the menaquinol binding loop of mycobacterial cytochrome bd oxidase

Molecular Diversity - Mycobacteria have shown enormous resilience to survive and persist by remodeling and altering metabolic requirements. Under stringent conditions or exposure to drugs,...     

Discovery of a Novel Mycobacterial F‐ATP Synthase Inhibitor and its Potency in Combination with Diarylquinolines

The F₁F_O‐ATP synthase is required for growth and viability of Mycobacterium tuberculosis and is a validated clinical target. A mycobacterium‐specific loop of the enzyme's rotary γ subunit plays a role in the coupling of ATP synthesis within the enzyme complex. We report the discovery of a novel antimycobacterial, termed GaMF1, that targets this γ subunit loop. Biochemical and NMR studies show that GaMF1 inhibits ATP synthase activity by binding to the loop. GaMF1 is bactericidal and is active against multidrug‐ as well as bedaquiline‐resistant strains. Chemistry efforts on the scaffold revealed a dynamic structure activity relationship and delivered analogues with nanomolar potencies. Combining GaMF1 with bedaquiline or novel diarylquinoline analogues showed potentiation without inducing genotoxicity or phenotypic changes in a human embryonic stem cell reporter assay. These results suggest that GaMF1 presents an attractive lead for the discovery of a novel class of anti‐tuberculosis F‐ATP synthase inhibitors.     

Gas chromatography with spectroscopic detectors.

In recent years, capillary gas chromatography (GC) with Fourier Transform Infrared (FT-IR) and/or mass spectral (MS) detection has become a primary analytical tool for qualitative and quantitative analysis of complex mixtures. Because of the wide range of applications, the analytical requirements have motivated a variety of chromatographic and detection developments. This review examines those, illustrating with applications that demonstrate the power of GC and multidimensional GC-MS, GC-FT-IR and GC-FT-IR-MS systems for solving a variety of analytical problems. In addition, the article discusses the integrated performance of such analytical systems with the aid of recent sample introduction and computer data analysis advances.     

Investigation on influence of dimpled surfaces on heat transfer enhancement and friction factor in solar water heater

Journal of Thermal Analysis and Calorimetry - The solar water heating system efficiently converts available solar energy into useful thermal energy. The collector is the predominant unit of the...     

Discovery of a Novel Mycobacterial F-ATP Synthase Inhibitor and its Potency in Combination with Diarylquinolines

The F₁F_O-ATP synthase is required for growth and viability of Mycobacterium tuberculosis and is a validated clinical target. A mycobacterium-specific loop of the enzyme's rotary γ subunit plays a role in the coupling of ATP synthesis within the enzyme complex. We report the discovery of a novel antimycobacterial, termed GaMF1, that targets this γ subunit loop. Biochemical and NMR studies show that GaMF1 inhibits ATP synthase activity by binding to the loop. GaMF1 is bactericidal and is active against multidrug- as well as bedaquiline-resistant strains. Chemistry efforts on the scaffold revealed a dynamic structure activity relationship and delivered analogues with nanomolar potencies. Combining GaMF1 with bedaquiline or novel diarylquinoline analogues showed potentiation without inducing genotoxicity or phenotypic changes in a human embryonic stem cell reporter assay. These results suggest that GaMF1 presents an attractive lead for the discovery of a novel class of anti-tuberculosis F-ATP synthase inhibitors.     

Comparison of Quasi Bang-Bang and Sliding Mode Controls for a DC Shunt Motor with Time Delay

We propose a hybrid fuzzy-linear controller for a nonlinearterminal voltage-controlled DC motor. The nonlinearity includes theeffect of magnetization saturation and a nonlinear fan load. The inputto the proposed Fuzzy Logic Controller (FLC) is an 'aggregate' errorbetween the required and the actual rpm and its time derivatives. TheFLC is designed to approximate a bang-bang controller and itscoefficients are chosen as a tradeoff between short rise time, smallsteady-state errors, and control chattering. The FLC performance wascompared to that of a well-known design; namely a sliding modecontroller with a boundary layer. Although the FLC design procedure iseasier, the performance of the two controllers are quite similar.Unfortunately, the FLC design was not robust enough to counter theeffect of large time delays introduced by the power electronicsinterface.