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Tandem purification of mouse IgM monoclonal antibodies produced in vitro using anion-exchange and gel fast protein liquid chromatography 总被引:1,自引:0,他引:1
P Clezardin N R Hunter I R MacGregor J L MacGregor D S Pepper J Dawes 《Journal of chromatography. A》1986,358(1):209-218
A tandem chromatographic procedure was used to isolate rapidly mouse IgM monoclonal antibodies produced by cultivation of hybridomas in vitro. Hybridoma culture supernatants containing mouse IgM monoclonal antibodies were first chromatographed on an anion-exchange Mono Q column connected to a fast protein liquid chromatography system. This anion-exchange step offers the advantage of obtaining IgM antibodies in a concentrated form. The IgM-rich fractions from the Mono Q column were then injected on a gel filtration Superose 6 column equilibrated with a low-ionic strength buffer and eluted with a high-ionic strength buffer. Assessment of the purity of isolated IgM monoclonal antibodies was performed by sodium dodecyl sulphate polyacrylamide gel electrophoresis together with a Coomassie Brillant Blue R 250 staining technique. Assessment of the immunoreactivity of isolated IgM monoclonal antibodies was performed by an enzyme linked immunosorbent assay using a solid phase adsorbed antigen against which IgM monoclonal antibodies were directed. The chromatographic procedures described allows the rapid isolation of mouse IgM monoclonal antibodies produced in vitro at a high degree of purity and in an immunoreactive state. 相似文献
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Astley MR Kataoka M Ford CJ Barnes CH Anderson D Jones GA Farrer I Ritchie DA Pepper M 《Physical review letters》2007,99(15):156802
We measure the electron escape rate from surface-acoustic-wave dynamic quantum dots (QDs) through a tunnel barrier. Rate equations are used to extract the tunneling rates, which change by an order of magnitude with tunnel-barrier-gate voltage. We find that the tunneling rates depend on the number of electrons in each dynamic QD because of Coulomb energy. By comparing this dependence to a saddle-point-potential model, the addition energies of the second and third electron in each dynamic QD are estimated. The scale ( approximately a few meV) is comparable to those in static QDs as expected. 相似文献
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T. Ferrus R. George C.H.W. Barnes N. Lumpkin D.J. Paul M. Pepper 《Physica B: Condensed Matter》2007,400(1-2):218-223
Sodium impurities are diffused electrically to the oxide-semiconductor interface of a silicon MOSFET to create an impurity band. At low temperature and at low electron density, the band is split into an upper and a lower sections under the influence of Coulomb interactions. We used magnetoconductivity measurements to provide evidence for the existence of Hubbard bands and determine the nature of the states in each band. 相似文献
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D. Bisello G. Busetto A. Castro M. Nigro M. Penzo L. Pescara M. Posocco P. Sartori L. Stanco Z. Ajaltouni A. Falvard J. Jousset B. Michel J. C. Montret A. Antonelli R. Baldini A. Calcaterra G. Capon M. Schioppa J. -E. Augustin G. Cosme F. Couchot B. Dudelzak F. Fulda G. Grosdidier B. Jean-Marie S. Jullian D. Lalanne V. Lepeltier F. Manè C. Paulot R. Riskalla Ph. Roy G. Szklarz DM Collaboration 《Zeitschrift fur Physik C Particles and Fields》1988,39(1):13-19
Thee + e ?→K + K ? cross section has been measured from about 750 events in the energy interval \(1350 \leqq \sqrt s \leqq 2400 MeV\) with the DM2 detector at DCI. TheK ± form factor |F F ±| cannot be explained by the ρ, ω, ? and ρ′(1600). An additional resonant amplitude at 1650 MeV has to be added as suggested by a previous experiment. 相似文献
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J. -E. Augustin G. Cosme F. Couchot B. Dudelzak F. Fulda B. Grelaud G. Grosdidier B. Jean-Marie S. Jullian D. Lalanne V. Lepeltier B. Louis F. Mané C. Paulot R. Riskalla Ph. Roy F. Rumpf G. Szklarz Z. Ajaltouni A. Falvard J. Jousset B. Michel J. C. Montret R. Baldini S. Calcaterra G. Capon D. Bisello G. Busetto S. Limentani M. Nigro L. Pescara M. Posocco P. Sartori L. Stanco DM Collaboration 《Zeitschrift fur Physik C Particles and Fields》1987,36(3):369-376
The radiative decayJ/ψ → γ π+ π? has been studied using the 8.6 millionJ/ψ produced in the DM2 experiment at the DCIe +e? storage rings at Orsay. The π+ π? mass spectrum shows a cleanf 2 (1270) signal, and the possible presence of two other states at thef 2 (1720) andf 4 (2030) masses. For thef 2 (1270), the branching ratio BR(J/ψ →γf)xBR(f→π+ π?) is measured to be (7.50±0.30±1.12)×10?4, and the spin analysis prefers theJ=2 assignment, with helicity parametersx=0.83±0.06 andy=0.01±0.06. The existence of higher mass states is discussed. 相似文献
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Pepper SE Bunker DJ Bryan ND Livens FR Charnock JM Pattrick RA Collison D 《Journal of colloid and interface science》2003,268(2):408-412
Technetium is a long-lived product of nuclear fission that readily forms the soluble pertechnetate anion [TcO(4)](-). Green rusts (layered hydrous oxides containing both Fe(II) and Fe(III) and with interlayer sulfate or carbonate anions) concentrate >99.8% of 99Tc, present as [TcO(4)](-), from aqueous solution, even in the presence of high concentrations of NaNO(3), a common constituent of radioactive waste streams. The mechanism of removal from solution is apparently reduction and formation of strong Tc(IV) surface complexes. X-ray absorption spectroscopy shows that [TcO(4)](-) is indeed reduced by reaction with both sulfate- and carbonate-form green rusts and is found in a TcO(2)-like environment. On contact with air, the green rusts oxidize to poorly crystalline goethite but the Tc environment is unchanged. There is no increase in Tc solubility associated with oxidation of the host green rust. This behavior suggests that green rusts may be useful in the treatment of Tc-containing waste streams, in groundwater cleanup, and in restricting Tc migration from repositories. 相似文献
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