Mapping of possible prion protein self-interaction domains using peptide arrays

Background  

The common event in transmissible spongiform encephalopathies (TSEs) or prion diseases is the conversion of host-encoded protease sensitive cellular prion protein (PrP^C) into strain dependent isoforms of scrapie associated protease resistant isoform (PrP^Sc) of prion protein (PrP). These processes are determined by similarities as well as strain dependent variations in the PrP structure. Selective self-interaction between PrP molecules is the most probable basis for initiation of these processes, potentially influenced by chaperone molecules, however the mechanisms behind these processes are far from understood. We previously determined that polymorphisms do not affect initial PrP^C to PrP^Sc binding but rather modulate a subsequent step in the conversion process. Determining possible sites of self-interaction could elucidate which amino acid(s) or amino acid sequences contribute to binding and further conversion into other isoforms. To this end, ovine – and bovine PrP peptide-arrays consisting of 15-mer overlapping peptides were probed with recombinant sheep PrP^C fused to maltose binding protein (MBP-PrP).     

Investigation of Alternative Length Scale Substitutions in Detached-Eddy Simulation

The objective of this work is the comparison of three different DES-style hybrid RANS/LES implementations based on the Wilcox k– model. The three variants are designed to investigate alternative methods of substitution of the DES length scale within the background Reynolds Averaged Navier-Stokes (RANS) model. Basis for comparison is provided by both the idealised case of the decay of isotropic turbulence (DIT) as well as the practical case of the massively separated, turbulent flow around an airfoil at high angle of attack. The results of the investigations are discussed in detail, the outcome of which is an emphasis of the importance of DIT as a method for calibration, as well as of the relative freedom with which alternative DES-inspired approaches can be implemented for flows of practical relevance.     

Studies on photoinduced effects in pulse-electrodeposited Ag/Hg-1212/CdSe hetero-nanostructures

Metal/superconductor/semiconductor (Ag/Hg-1212/CdSe) hetero-nanostructures have been fabricated using pulse-electrodeposition technique and are characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy (SEM) studies. The junction capacitance of Ag/Hg-1212, Hg-1212/CdSe and Ag/Hg-1212/CdSe heterojunctions is measured in dark and under laser irradiation at room temperature. The nature of the junction formed and built-in-junction potentials were determined. The increase in carrier concentration across the junction due to photo-irradiation has been observed.     

Effect of laser irradiation of C-V characteristics of electrodeposited Ag/Tl-2223/CdSe hetero-nanostructures

One of the innovative technological directions for the high-temperature superconductors has been persued by fabricating the heteroepitaxial multilayer structures such as superconductor-semiconductor heterostructures. In the present investigation, metal/superconductor/semiconductor (Ag/Tl-2223/CdSe) hetero-nanostructures have successfully been fabricated using dc electrodeposition technique and were characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy (SEM) studies. The measurement of junction capacitance as a function of biasing voltage was used for the estimation of junction built-in-potential (V _D) and to study the charge distribution in a heterojunction. The Mott-Schottky plots were measured for each junction in dark and under the photo-irradiation. The effect of laser irradiation on C-V characteristics of hetero-nanostructure has been studied.     

A measurement of the energy dependence of the inclusive yield of high-Pt events triggered by a large solid angle calorimeter

Large aperture calorimeter measurements of high transverse momentum events from 100, 200 and 340 GeV pp collisions are reported. Yields are higher by one to two orders of magnitude than the yield expected from uncorrelated statistical fluctuations in multiparticle events. The exponential slopes of the yields versus x_t exhibited a systematic change with $\text{s}\text{(x}{}_{\mathrm{<mtext>t</mtext>}}\text{= 2P}{}_{\mathrm{<mtext>t</mtext>}}\text{/}\text{s}\text{)}$. The events are shown to be more diffuse than pure jets as produced in e⁺e^? collisions. However, these events are consistent with a QCD-inspired 4-jet model.     

Structure in the K+-nucleon I = 0 total cross section below 1.1 GeV/c

Measurements have been carried out of total cross sections of positive kaons on hydrogen and deuterium to a statistical precision of better than ± 0.1 mb for most points in the range of laboratory momentum from 410 to 1065 MeV/c in intervals of approximately 50 MeV/c. A very broad elastic structure in the I = 0 state is inferred.