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1.
Guralchuk GY Sorokin AV Katrunov IK Yefimova SL Lebedenko AN Malyukin YV Yarmoluk SM 《Journal of fluorescence》2007,17(4):370-376
Optical spectroscopy experiments were used to study the features of cyanine dye 3,3′-dimethyl-9-(2-thienyl)-thiacarbocyanine
iodide (L-21) aggregation in binary solutions DMF:Tris–HCl buffer (pH = 8) containing nucleic acids (DNA or RNA). The appearance
of absorption and luminescence bands associated with J-aggregates and dimers that are formed within the minor groove of DNA
has been observed. The model of L-21 J-aggregate structure is proposed. It has been found that dimers are the building blocks
of L-21 J-aggregates. Disorientation in dimers caused by the minor groove curvature is reason of observation of Davydov splitting
in absorption spectrum of L-21 J-aggregates. In the solution containing DNA the absorption and luminescence bands of L-21
J-aggregates exhibit the specific properties that allows the dye L-21 to be used as a fluorescent probe for DNA detection. 相似文献
2.
3.
Lebedenko AN Guralchuk GY Sorokin AV Yefimova SL Malyukin YV 《The journal of physical chemistry. B》2006,110(36):17772-17775
Using fluorescent microscopy and microspectroscopy, optical properties and morphology transformations in individual pseudoisocyanine (PIC) J-aggregates in aqueous electrolyte solutions have been explored. A stringlike structure of J-aggregates with a string diameter much less than 1 microm has been observed. Photodestruction of the strings under short-wavelength excitation has been revealed. Rodlike PIC crystallites, about 1 microm in diameter, have been observed with time. The fluorescence spectrum of rodlike crystallites has been found to differ from that of stringlike J-aggregate and from PIC crystal powder spectra. The crystallites are very stable, and their photodestruction has not been observed under any excitation conditions. It has been found that rodlike crystallites in contrast to stringlike J-aggregates possess optical waveguide properties. The luminescence of crystallites can be observed only at the excitation spot and at butt ends located up to hundreds of micrometers from the excitation spot. 相似文献
4.
N. V. Znamenskii P. O. Maksimchuk Yu. V. Malyukin A. A. Masalov V. V. Semin’ko A. Yu. Shashkov 《Journal of Experimental and Theoretical Physics》2013,116(4):579-586
Stationary and time-resolved spectroscopic methods are used to show that the impurity ions in Y2SiO5:Pr3+ and YPO4:Pr3+ nanocrystals are distributed nonuniformly. This nonuniform distribution is found to be caused by the temperature-dependent segregation of Pr3+ ions near the surface of a nanocrystal. The motion of the activator ions from the bulk of a nanocrystal to the near-surface layer is traced when the activator concentration and the heat-treatment parameters are varied over wide ranges, and the main parameters of this effect (impurity redistribution intensity and time, diffusion coefficient) are estimated. 相似文献
5.
S. L. Yefimova G. Ya. Gural’chuk A. V. Sorokin Yu. V. Malyukin I. A. Borovoy A. S. Lubyanaya 《Journal of Applied Spectroscopy》2008,75(5):658-663
Interactions between the cationic polymethine dyes 3,3′-diethyloxacarbocyanaine iodide (DiOC2), 3,3′-dioctadecyloxacarbocyanine iodide (DiOC18), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in the nano-scale volume of anionic sodium
dodecylsulfate (SDS) micelles have been studied using optical spectroscopy techniques. The solubilization of pairs of dye
molecules (DiOC18/DiI or DiOC2/DiI) within SDS micelles was monitored by Forster resonance energy transfer (FRET) between the dyes in each pair. The influence
of the hydrophobicity of the dyes DiOC2 and DiOC18 on the efficiency of their binding to SDS micelles and, consequently, on the efficiency of FRET between DiOC18 and DiI or DiOC2 and DiI fluorophors has been analyzed. It has been shown that hydrophobic interactions in addition to electrostatic interactions
are of key importance for the binding between the positively charged dyes and negatively charged surfactant micelles.
Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 75, No. 5, pp. 646–652, September–October, 2008. 相似文献
6.
NL Asfandiarov AI Fokin VG Lukin EP Nafikova GS Lomakin VS Fal'ko YV Chizhov 《Rapid communications in mass spectrometry : RCM》1999,13(12):1116-1123
A series of anthraquinone (C(14)O(2)H(8)) derivatives has been studied by means of electron capture negative ion mass spectrometry (ECNI-MS), photoelectron spectroscopy (PES), and AM1 quantum chemical calculations. Mean lifetimes of molecular negative ions M(-.) (MNI) have been measured. The mechanism of long-lived MNI formation in the epithermal energy region of incident electrons has been investigated. A simple model of a molecule (a spherical potential well with the repulsive centrifugal term) has been applied for the analysis of the energy dependence of cross sections at the first stage of the electron capture process. It has been shown that a temporary resonance of MNI at the energy approximately 0.5 eV corresponds to a shape resonance with lifetime 1-2.10(-13) s in the f-partial wave (l = 3) of the incident electron. The next resonant state of MNI at the energy approximately 1.7 eV has been associated with the electron excited Feshbach resonance (whose parent state is a triplet npi* transition). In all cases the initial electron state of the MNI relaxes into the ground state by means of a radiationless transition, and the final state of the MNI is a nuclear excited resonance with a lifetime measurable on the mass spectrometry timescale. Copyright 1999 John Wiley & Sons, Ltd. 相似文献
7.
R. S. Borisov B. V. Grinev Yu. V. Malyukin B. I. Minkov N. V. Znamenskii É. A. Manykin D. V. Marchenko E. A. Petrenko 《Journal of Experimental and Theoretical Physics》1999,88(2):385-391
The results of an experimental investigation of low-temperature optical spectra and phase relaxation of electronic excitations
of Pr3+ impurity ions in a Y2SiO5 crystal are reported. It is established that at low temperatures spectral lines are broadened by a mechanism that is uncharacteristic
for crystals and is due to the interaction of impurity ions with two-level systems. The constants characterizing the interaction
of Pr3+ impurity ions with phonons and two-level systems are found.
Zh. éksp. Teor. Fiz. 115, 704–715 (February 1999) 相似文献
8.
Svetlana L. Yefimova Irina Yu. Kurilchenko Tatyana N. Tkacheva Nataliya S. Kavok Igor N. Todor Nataliya Yu. Lukianova Vasyl F. Chekhun Yuriy V. Malyukin 《Journal of fluorescence》2014,24(2):403-409
We report the Förster resonance energy transfer (FRET)-labeling of liposomal vesicles as an effective approach to study in dynamics the interaction of liposomes with living cells of different types (rat hepatocytes, rat bone marrow, mouse fibroblast-like cells and human breast cancer cells) and cell organelles (hepatocyte nuclei). The in vitro experiments were performed using fluorescent microspectroscopic technique. Two fluorescent dyes (DiO as the energy donor and DiI as an acceptor) were preloaded in lipid bilayers of phosphatidylcholine liposomes that ensures the necessary distance between the dyes for effective FRET. The change in time of the donor and acceptor relative fluorescence intensities was used to visualize and trace the liposome-to-cell interaction. We show that FRET-labeling of liposome vesicles allows one to reveal the differences in efficiency and dynamics of these interactions, which are associated with composition, fluidity, and metabolic activity of cell plasma membranes. 相似文献
9.
Strong temperature controlled segregation of doped ions in Y2SiO5:Pr3+ nanocrystals detected by spectroscopic techniques is reported. The elastic interactions stimulate Pr3+ segregation thus leading to non-uniform distribution of doped ions, pair formation and, as a consequence, to abnormal low threshold of luminescence concentration quenching for Y2SiO5:Pr3+ nanocrystals. 相似文献
10.
R. S. Grinyov A. V. Sorokin G. Ya. Gural’chuk S. L. Efimova I. A. Borovoi Yu. V. Malyukin 《Theoretical and Experimental Chemistry》2009,45(1):58-62
We used a squarylium dye SQ as a specific exciton trap for J aggregates of the amphiphilic cyanine dye amphi-PIC. The exciton transport parameters in amphi-PIC J aggregates were estimated using a modified Stern–Volmer equation. We found that SQ quenches 50% of the luminescence of amphi-PIC
J aggregates for a ratio of 1 SQ molecule per 80 amphi-PIC molecules.
Translated from Teoreticheskaya i éksperimental’naya Khimiya, Vol. 45, No. 1, pp. 50–53, January–February, 2009. 相似文献