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Amina Chnirheb Mourad Harir Basem Kanawati Agnes Fekete Mohammed El Azzouzi Norbert Hertkorn Philippe Schmitt-Kopplin 《Analytical and bioanalytical chemistry》2010,398(5):2325-2334
The hydrolysis of the herbicide safener mefenpyrdiethyl (1-(2, 4-dichlorophenyl)-4, 5-dihydro-5-methyl-1H-pyrazole-3,5-dicarboxylic acid diethyl ester) was investigated in aqueous solutions in the pH range from 2 to 9 and the temperature
range from 298 to 323 K. The kinetics of hydrolysis were pseudo first order and were found to be strongly pH and temperature
dependent. While near-constant in acidic medium, the hydrolysis rates strongly increased in alkaline pH, and total hydrolysis
was observed at pH 11. Two main hydrolysis products, mefenpyrethyl (monoester) and mefenpyr (dicarboxylic acid) were isolated
by ultrahigh-pressure liquid chromatography (UHPLC) and characterized using high-resolution Fourier transform ion cyclotron
resonance mass spectroscopy (ICR-FT/MS) as well as 1H, 13C and 2D NMR analyses. Additionally, a density functional theory (DFT) investigation explained the stability of the pesticide
at pH 7 and the high reactivity of the pesticide in alkaline medium. The key nucleophilic reaction partner is hydroxyl ions
instead of neutral water molecules. Furthermore, the calculated activation barrier for hydrolysis in alkaline medium is in
agreement with the extrapolated and experimentally determined activation barrier at pH 14. 相似文献
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Andras Gaspar Mourad Harir Norbert Hertkorn Philippe Schmitt‐Kopplin 《Electrophoresis》2010,31(12):2070-2079
Free‐flow electrophoresis (FFE), a preparative free zone electrophoretic method, was used offline in conjunction with ultrahigh‐resolution FT/ion cyclotron resonance ‐MS to resolve the complexity of Suwannee River fulvic acid (SRFA). Before MS, the FFE separation conditions and the compatibility with ESI were optimized. The constituents in SRFA were effectively separated based on their charge states and sizes. The obtained mass spectra were compared by means of van Krevelen diagrams and the calculated aromaticity indices of the individual constituents were used to describe the distribution of aromatic/unsaturated structures across the FFE‐fractionated samples. The consolidated number of ions observed within the individual SRFA fractions were much higher than those of the bulk samples alone, demonstrating extensive ion suppression effects in bulk SRFA likely also operating in the analysis of complex biogeochemical mixtures in flow injection mode. The FFE approach allows for producing sizable amounts of sample from dilute solutions, which can be easily fractionated into dozens of individual samples with the possibility of further in‐depth characterization. 相似文献
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Gaspar A Harir M Lucio M Hertkorn N Schmitt-Kopplin P 《Rapid communications in mass spectrometry : RCM》2008,22(20):3119-3129
Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) was applied to identify boric acid(B degrees)/borate(B-) complexes in a monomolecular model system, composed of aqueous caffeic acid and boric acid/borate solutions in various concentration ratios at pH 9.2. Using negative mode electrospray ionization as a 'soft' ionization technique, clusters of polyborate were detected beside the well-known BL degrees , BL- and BL2- complexes. An algorithm for the detection of boron complexes, based on their accurate mass and isotope patterns, is proposed which enabled the assignment of elemental compositions/structural formulae of boron/ligand complexes. We present experimental evidence of self-oligomerization of up to six borate units with caffeic acid, resulting in stable covalently bound polyborate-polyol complexes. 相似文献
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Capillary electrokinetic fractionation mass spectrometry (CEkF/MS): Technology setup and application to metabolite fractionation from complex samples coupled at‐line with ultrahigh‐resolution mass spectrometry
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Yu He Mourad Harir Guonan Chen Regis D. Gougeon Lan zhang Xiayang Huang Philippe Schmitt‐Kopplin 《Electrophoresis》2014,35(14):1965-1975
Capillary electrokinetic fractionation (CEkF) is investigated as a new, simple, and robust approach for semipreparative and analytical sample analysis based on pKa‐dependant pH‐driven electrophoretic mobility. CEkF was optimized with contactless conductivity detection and conducted with 10 kV reverse voltage for 10 min, then coupled on/at‐line to ESI/MS. We propose a semi‐empirical model with 14 representative compounds based on the correlation between sample/medium pH regulating the partial charge, the electrokinetic loading of the capillary and intensity (I) of analytes. According to the model, an empirical function (I = f (pH)) could be derived to calculate the acid dissociation constant (pKa) of various model compounds based on their pH‐dependant MS intensity profiles with the RSD < 4.05. Using the ultrahigh‐resolution of ion cyclotron resonance Fourier transform MS, the pKa model was further illustrated in real samples into the structure prediction of important compounds in wine over two vintages. The established CEkF was successfully used to selectively fractionate sulfur compounds from the complex wine samples at pH 1.66. The proposed CEkF approach should allow in the future the simultaneous pKa evaluation of multiple constituents without complicated separation out of a complex mixture in metabolomics or environmental chemistry. 相似文献
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Desislava Jivkova Ganesan Sathiyanarayanan Mourad Harir Norbert Hertkorn Philippe Schmitt-Kopplin Ghislain Sanhaji Sylvain Fochesato Catherine Berthomieu Alain Heyraud Wafa Achouak Catherine Santaella Thierry Heulin 《Molecules (Basel, Switzerland)》2022,27(21)
The current study examines the desiccation-resistant Ramlibacter tataouinensis TTB310T as a model organism for the production of novel exopolysaccharides and their structural features. This bacterium is able to produce dividing forms of cysts which synthesize cell-bound exopolysaccharide. Initial experiments were conducted on the enrichment of cyst biomass for exopolysaccharide production under batch-fed conditions in a pilot-scale bioreactor, with lactate as the source of carbon and energy. The optimized medium produced significant quantities of exopolysaccharide in a single growth phase, since the production of exopolysaccharide took place during the division of the cysts. The exopolysaccharide layer was extracted from the cysts using a modified trichloroacetic acid method. The biochemical characterization of purified exopolysaccharide was performed by gas chromatography, ultrahigh-resolution mass spectrometry, nuclear magnetic resonance, and Fourier-transform infrared spectrometry. The repeating unit of exopolysaccharide was a decasaccharide consisting of ribose, glucose, rhamnose, galactose, mannose, and glucuronic acid with the ratio 3:2:2:1:1:1, and additional substituents such as acetyl, succinyl, and methyl moieties were also observed as a part of the exopolysaccharide structure. This study contributes to a fundamental understanding of the novel structural features of exopolysaccharide from a dividing form of cysts, and, further, results can be used to study its rheological properties for various industrial applications. 相似文献
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Basem Kanawati Mourad Harir Philippe Schmitt-Kopplin 《International journal of mass spectrometry》2009,288(1-3):6-15
Diuron (3-(3,4-dichlorophenyl)-1,1-dimethylurea), a common herbicide from phenyl urea class, was investigated by studying the formation of several negative ions [M−H]− in the gas phase and the fragmentation behaviour of the thermodynamically most probably formed isomeric anions upon linear ion acceleration/collision experiments. The collision induced dissociation experiments (CID) were carried out in a hexapole–quadrupole–hexapole hybrid system coupled to 12 T magnet with infinity ICR cell for high resolution measurements. Two distinctive main pathways were observed in the MS/MS spectrum. Sustained off-resonance irradiation (SORI) experiments inside the ICR cell reinforce the fragmentation channels obtained from linear ion acceleration experiments. The fragmentation pathways were also completely investigated by the use of B3LYP/6-311+G(2d,p)//B3LYP/6-31+G(d) level of theory. Elimination of dimethylamine takes place in a two-step process, by which two successive 1,3 proton shifts occur. The second 1,3 proton shift is concerted with the departure of dimethylamine. The driving force for the (CH3)2NH elimination is the formation of isocyanate group. The formed primary product ion can further decompose to release HCl through a new transition state. A stable new aromatic product ion is formed with 10π electrons. Loss of C3H5NO neutral from another anionic isomer of the precursor ion was also observed and is characteristic for the amide terminal of the diamide functional group. A concerted mechanism is proposed, by which N–C bond breakage and cyclization of the eliminated neutral fragment C3H5NO takes place simultaneously to form 1-methyl-aziridin-2-one. 相似文献
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CE-MS has gained further attention as a multidimensional analytical approach. The number of publications dealing with this technique is still increasing on the level of application as well as method development-oriented approaches. Additionally, 15 reviews were published the last two years focusing on CE-MS. An overview of all papers found to have been published within 2005 and 2006 is given in tabulated form. Additionally, four promising technical trends are chosen to be presented in detail: (i) chip-based CE-MS, (ii) capillary coatings in CE-MS, (iii) new trends in CEC-MS and (iv) the application of 2-D CE-MS. 相似文献