排序方式: 共有35条查询结果,搜索用时 15 毫秒
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Givens RS Timberlake GT Conrad PG Yousef AL Weber JF Amslinger S 《Photochemistry and photobiology》2003,78(1):23-29
On the basis of the earlier examples of diazopyruvoyl (DAP) groups reported by Lawton for covalent binding and cross-linking of proteins and oligopeptides and our recent demonstration that a coumaryl diazopyruvamide was used to label Type-I collagen, we have extended our investigations to the synthesis and cross-linking capabilities of a bis-DAP polyethylene glycol to cross-link Type-I collagen. The new photoactivated cross-linking agent, N,N'-bis(3-diazopyruvoyl)-2,2'-(ethylenedioxy)bis(ethylamine) (DPD, 2), has been designed and synthesized specifically to "weld" collagenous tissues by cross-linking Type-I collagen. A working model for the photochemical welding studies of collagenous tissues was developed using gelatin strips (gel strips) composed of denatured Type-I collagen. Gel strips are transparent to near-UV and visible light, uniform in thickness, and have reproducible composition. Furthermore, the availability of nucleophilic amine sites in gel strips was demonstrated by reaction with o-phthalaldehyde, producing a fluorescent derivative of the protein. Gel strips were coated with a solution of DPD in chloroform 7 irradiated at 320-390 nm, and the resulting bonded gel strips were tested for the strength of the weld. The welds were generally brittle and had average tensile strengths that exceeded 100 N/cm2. Welds were not formed in the absence of light or DPD. Scanning electron microscopy studies revealed a pockmarked surface from severed welds. Welds of rabbit Achilles tendon were also obtained using the tethered diazopyruvamide. These welds were much weaker, having an average tensile strength of 11.95 N/cm2 for DPD-2,2'-ethylenedioxy(bis)ethylamine comonomers in the cross-linking reaction. In both studies the welds obtained by this method were significantly stronger than the controls. 相似文献
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M.Parker Givens 《Optics Communications》1982,41(3):145-148
It is known that when a converging, monochromatic, spherical wave is diffracted at a circular aperture, the point of maximum intensity may not be at the geometric focus of the incident wave, but may be located closer to the aperture. In the present note this effect is calculated using somewhat simpler mathematical procedures than have been used in previous publications. The result is interpreted as the combined effect of diffraction and the inverse square law. 相似文献
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Timberlake GT Yousef AL Chiles SR Moses RA Givens RS 《Photochemistry and photobiology》2005,81(5):1180-1185
Photoactivated bis-diazopyruvamide-N,N'-bis(3-diazopyruvoyl)-2,2'-(ethylenedioxy)bis-(ethylamine), (DPD)-was previously shown to bond materials containing type I collagen. However, tensile strength of bonded collagenous tissue ( approximately 78% water) was low compared with that of dehydrated collagenous gelatin ( approximately 14% water). Here we investigated the role of water in corneal tissue bond strength and in bonding corneal tissue to glass. Bonding corneal tissue to glass may be of value in surgically anchoring keratoprostheses to corneas to alleviate problems with extrusion. Bovine corneal samples were lyophilized for various times resulting in tissue hydrations of zero (no water content) to approximately 3.7 (normal water content). The lyophilized corneal tissue was bonded to solid gelatin sheets, to other corneal samples and to glass using 0.3M DPD in chloroform. Control runs used chloroform only. Samples were irradiated with 100 or 200 J of 320-500 nm light. Strong bonds formed with all three materials when corneal tissue hydration was =1. No bonds or extremely weak bonds formed when tissue hydration levels were >1. No bonding occurred with chloroform alone. Formation of strong bonds only occurs with hydration levels =1 because corneal collagen fibrils are tightly packed and close enough to cross-link with the 1.78 nm long DPD. 相似文献
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Stuart A Givens GD Walker LJ Elangovan S 《The Journal of the Acoustical Society of America》2006,119(4):1946-1949
The purpose of this study was to examine temporal resolution in normal-hearing preschool children. Word recognition was evaluated in quiet and in spectrally identical continuous and interrupted noise at signal-to-noise ratios (S/Ns) of 10, 0, and -10 dB. Sixteen children 4 to 5 years of age and eight adults participated. Performance decreased with decreasing S/N. At poorer S/Ns, participants demonstrated superior performance or a release from masking in the interrupted noise. Adults performed better than children, yet the release from masking was equivalent. Collectively these findings are consistent with the notion that preschool children suffer from poorer processing efficiency rather than temporal resolution per se. 相似文献
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The interassociates of the free commutative semigroup on n generators, for n > 1, are identified. For fixed n, let (S, ·)
denote this semigroup. We show that every interassociate can be written in the form
, depending only on a n-tuple
. Next, if
and
are isomorphic interassociates of (S, ·) such that
, for xii and xj in the generating set of S, then
. Moreover,
if and only if
is a permutation of
. 相似文献
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[equation--see text] Introducing 3-methoxy or 3,5-dimethoxy substituents on the 4-hydroxyphenacyl (pHP) photoremovable protecting group has been explored with two excitatory gamma-amino acids, L-glutamic acid and gamma-amino butyric acid (GABA). These substituents significantly extend the absorption range of the pHP chromophore, e.g., the tail of absorption bands of 2a,b extend above 400 nm, well beyond the absorptions of aromatic amino acids and nucleotides. Irradiation releases the amino acids with rate constants of approximately 10(7) s(-)(1) and appearance efficiencies (Phi(app)) of 0.03-0.04. The photoproducts are formed through the pHP excited triplet and are primarily products of photoreduction and photohydrolysis. 1a,b also rearranged to the phenylacetic acid 3. 相似文献
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Maria D.M.C. Ribeiro da Silva Mnica A.A. Vieira Chelsea Givens Stephanie Keown William E. Acree Jr. 《Thermochimica Acta》2006,450(1-2):67-70
The standard (p° = 0.1 MPa) molar enthalpies of formation of crystalline 2,3,5-trimethylpyrazine-1,4-dioxide and 2,3,5,6-tetramethylpyrazine-1,4-dioxide were measured, at T = 298.15 K, by static bomb calorimetry and the standard molar enthalpies of sublimation, at T = 298.15 K, were obtained from Calvet microcalorimetric measurements. These values were used to derive the respective standard molar enthalpies of formation in gaseous phase. The mean N–O bond dissociation enthalpy has been calculated for both compounds. 相似文献
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M. E. Givens C. A. Zmudzinski R. P. Bryan J. J. Coleman 《Fiber and Integrated Optics》2013,32(4):343-352
Graded barrier quantum well heterostructure (GBQWH) broad area lasers have been shown to be capable of high power pulsed and cw operation. In this article, we consider several operational characteristics and design issues associated with broad area graded barrier quantum well heterostructure lasers grown by metalorganic chemical vapor deposition. In particular, the effect of junction heating on emission wavelength for cw device operation and the effects of various buffer layer structures on the material properties and device characteristics of GBQWH structures are addressed. Typical results for high power operation of uncoated broad area laser diodes are also outlined. 相似文献