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1.
A novel species of DNA--protein conjugate was synthesized by chemically linking DNA oligonucleotides to Aequorea victoria green fluorescent protein mutant EYFP. An additional cysteine was added to the C-terminus of the EYFP by genetic engineering and used to covalently attach amino-modified oligonucleotide with the aid of the heterobifunctional crosslinker sSMCC. EYFP maintained its fluorescence upon conjugation. The oligonucleotide provides an additional binding site to the fluorescent protein, and hence, the EYFP conjugate could be specifically hybridized with both complementary DNA-protein conjugates in-solution as well as immobilized at capture oligonucleotides attached to a solid substrate. These studies are paving the way for future applications in the self-assembly of photoactive supramolecular complexes, such as artificial light-harvesting systems. 相似文献
2.
The "epimerisation" of UDP-GlcNAc to ManNAc, the first step in the biosynthesis of sialic acids, is catalyzed by UDP-GlcNAc 2-epimerase. In this paper we report the synthesis of transition state based inhibitors of this enzyme. To mimic the assumed first transition state of this reaction (TS 1), we designed and synthesized the novel UDP-exo-glycal derivatives 1-4. We also report herein the synthesis of 5 and 6, the first C-glycosidic derivatives of 2-acetamidoglucal, and the synthesis of the ketosides 7 and 8, which were designed as bis-substrate analogue and bis- product analogue, respectively, to mimic the second step of the reaction via the assumed second transition state TS 2. 相似文献
3.
Comparison of microwave-assisted acid leaching techniques for the determination of heavy metals in sediments, soils, and sludges 总被引:2,自引:0,他引:2
Microwave-assisted EPA method 3051 for nitric acid leaching of environmentally key elements from sediments, soils, and sludges
was tested, and the influence of leaching temperature and time on element recovery for an estuarine sediment (CRM 277) was
investigated. The extraction efficiencies for four certified reference materials applying EPA method 3051, an optimized nitric
acid procedure, and an aqua regia (HCl/HNO3 3:1) procedure were compared. Digestions were carried out in a high-pressure microwave system offering simultaneous temperature
and pressure control for all digestion vessels employed. Eight elements (Cd, Co, Cr, Cu, Hg, Ni, Pb, and Zn) were determined
by ICP-AES and ICP-MS. Extraction efficiency strongly depended on the applied leaching parameters and varied for certain elements
among different materials when a nitric acid procedure was applied. In general, element recoveries obtained from the aqua regia procedure were superior to those obtained from nitric acid procedures and showed good agreement with the 95% confidence interval
of the certified value for most of the elements investigated.
Received: 27 March 1998 / Revised: 29 June 1998 / Accepted: 3 July 1998 相似文献
4.
Louis Dressler Hendrik Nicolai Senda Agrebi Florian Ries Amsini Sadiki 《Entropy (Basel, Switzerland)》2022,24(5)
This contribution presents a straightforward strategy to investigate the entropy production in stratified premixed flames. The modeling approach is grounded on a chemistry tabulation strategy, large eddy simulation, and the Eulerian stochastic field method. This enables a combination of a detailed representation of the chemistry with an advanced model for the turbulence chemistry interaction, which is crucial to compute the various sources of exergy losses in combustion systems. First, using detailed reaction kinetic reference simulations in a simplified laminar stratified premixed flame, it is demonstrated that the tabulated chemistry is a suitable approach to compute the various sources of irreversibilities. Thereafter, the effects of the operating conditions on the entropy production are investigated. For this purpose, two operating conditions of the Darmstadt stratified burner with varying levels of shear have been considered. The investigations reveal that the contribution to the entropy production through mixing emerging from the chemical reaction is much larger than the one caused by the stratification. Moreover, it is shown that a stronger shear, realized through a larger Reynolds number, yields higher entropy production through heat, mixing and viscous dissipation and reduces the share by chemical reaction to the total entropy generated. 相似文献
5.
6.
Wolfgang Schreier Tobias Schrader Florian Roller Peter Gilch Wolfgang Zinth et al. 《Applied physics. B, Lasers and optics》1994,58(1):A6-A8
Call for papers
Time-resolved vibrational spectroscopy 相似文献7.
Florian Enescu 《Journal of Pure and Applied Algebra》2003,178(2):159-167
It is well known that nice conditions on the canonical module of a ring have a strong impact in the study of strong F-regularity and F-purity. For example, in the Gorenstein (or even -Gorenstein) case strong F-regularity is equivalent to weak F-regularity, and it is conjectured that this is true in general. In this note, we will show how to use the double cover of a ring to obtain sufficient conditions for strong F-regularity and F-purity. Our results involve a closure operation for a pair of ideals that could be of relevance for the conjecture mentioned above. 相似文献
8.
In the present paper we propose three new methods for computing sequences of enclosing intervals for a zero of a real function without convexity assumptions.The new methods have been tested on a series of published examples. The numerical experiments show that our methods are comparable in terms of efficiency with the well-known algorithms of Dekker and Brent.The present paper was written when this author was visiting the University of Karlsruhe. He would like to acknowledge the support provided by the University of Karlsruhe. 相似文献
9.
Florian Enescu 《Proceedings of the American Mathematical Society》2003,131(11):3379-3386
The notion of stability of the highest local cohomology module with respect to the Frobenius functor originates in the work of R. Hartshorne and R. Speiser. R. Fedder and K.-i. Watanabe examined this concept for isolated singularities by relating it to -rationality. The purpose of this note is to study what happens in the case of non-isolated singularities and to show how this stability concept encapsulates a few of the subtleties of tight closure theory. Our study can be seen as a generalization of the work by Fedder and Watanabe. We introduce two new ring invariants, the -stability number and the set of -stable primes. We associate to every ideal generated by a system of parameters and an ideal of multipliers denoted and obtain a family of ideals . The set is independent of and consists of finitely many prime ideals. It also equals prime ideal such that is -stable. The maximal height of such primes defines the -stability number.
10.
For tilt angles smaller than the meridional ray coupling condition previously investigated [S. F. Morse et al., J. Acoust. Soc. Am. 103, 785-794 (1998)], flexural helical waves on cylindrical shells can significantly enhance the backscattering. These contributions are compared and modeled here for an empty cylinder. Experiments using tone bursts were performed on a tilted stainless steel shell to investigate the contributions caused by flexural leaky Lamb waves above the coincidence frequency of the shell. In some of the measurements the tone bursts were of sufficient duration to superpose helical wave contributions of successive circumnavigations, along with the meridional contribution near the critical tilt, to arrive at a quasi-steady-state backscattering amplitude for the cylinder. These measurements are compared with an approximate numerical partial-wave series solution and a ray theory as a function of the tilt angle. The data for ka = 20 follow the basic shape of the ray theory and the relevant features of the partial-wave model. They illustrate the importance of the interference of successive helical wave contributions. Measurements (also as a function of the tilt angle) using tone bursts that were sufficiently short to separate the earliest helical wave contribution from later contributions also support the ray theory. 相似文献