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For the detection of the cystic fibrosis protein (CFP) in serum of cystic fibrosis (CF) carriers, thin-layer polyacrylamide gel isoelectric focusing proved inappropriate as a diagnostic test, but was useful for screening fractions on purification of CFP by chromatofocusing on a Mono P column. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis an Mr 12,000 protein (P12) was found in most CFP-positive sera, indicating good correlation between these two CF-associated proteins. Detection of the P12 protein by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was well reproducible and less delicate than IEF. The technique was also used to purify P12 from serum by two successive preparative electrophoresis steps in a 7.5-15% gradient and 15% homogeneous gel. The use of silver staining revealed that P12, which was present in all sera of CF patients and carriers with variable intensities, was also present in trace amounts in normal sera. 相似文献
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Figarella CF Benkadda S Beyer P Garbet X Voitsekhovitch I 《Physical review letters》2003,90(1):015002
Effects of externally imposed and self-generated poloidal flows on turbulent transport in the edge region of a tokamak are investigated using 3D nonlinear global simulations of resistive pressure-gradient-driven turbulence. Transport reduction is found to be due to synergetic changes in the fluctuation amplitude and in the dephasing of the fluctuations. A scaling of the fluctuation level and turbulent diffusivity with E x B flow shear strength is deduced from these simulations. These scalings agree with recent experimental observations on edge biasing as well as with analytical models. 相似文献
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I. Voitsekhovitch X. Garbet S. Benkadda P. Beyer C. F. Figarella 《Czechoslovak Journal of Physics》2002,52(10):1097-1106
Statistical properties of ion temperature gradient driven turbulence and anomalous flux are analysed in plasmas with low and
improved energy confinement. The turbulence is simulated using the three dimensional fluid code, where the source is prescribed
and the turbulence and temperature profile evolve self-consistently. The transition to the improved core confinement with
an internal transport barrier is obtained by increasing the heating power. It is found that the momentums of the probability
distribution function of flux fluctuations reduce to their Gaussian values when the transport barrier on the temperature profile
forms. The formation of the oscillating structure with the properties of linear wave has been found at very high heating power.
Presented at 5th Workshop “Role of Electric Fields in Plasma Confinement and Exhaust”, Montreux, Switzerland, June 23–24,
2002. 相似文献
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María L. Novoa Yelisbeth Escalante Liliana Maldonado Iván Galindo-Castro Annamil Álvarez Katherine Figarella 《Natural product research》2015,29(3):207-212
13,14-Dihydroxy-8,11,13-podocarpatrien-7-one (1) and a series of ring C aromatic diterpene derivatives were synthesised from (+)-manool (4) and evaluated for their cytotoxic, leishmanicidal and trypanocidal activities. Our results indicated that compound 1 and other podocarpane-type intermediates are cytotoxic. Cleavage of C6–C7 bond of compound 7 improved cytotoxic activity, indicating that, in particular, the 6,7-seco-podocarpane-type compound 20 might serve as a lead compound for further development. 相似文献
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In this paper a proof of existence and non-existence of theconically self-similar free-vortex solutions to the Navier-Stokesequations, originally found by Yih et al. (1982, Phys. Fluids.25, 2147-2158), is presented. This proof clearly establishesthat these solutions do not have any kind of singularity atthe symmetry axis. This analysis gives considerably improvedexistence and non-existence bounds and it is shown that thesebounds are close to optimal in the low-swirling limit. Thisapproach links the questions of existence and non-existencefor the swirling case and for the non-swirling case. The proof,which is an extension of techniques developed by Serrin (1972,Phil. Trans. R. Soc. Lond. 271, 325-360), is based on Schauder'sFixed Point Theorem and is, therefore, non-constructive. Therefore,the paper ends with a brief discussion of the question of howto compute the conically self-similar free-vortex solutionsto the Navier-Stokes equations. 相似文献
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