Spatial distribution and formation of nitrate radical NO<Stack><Subscript>3</Subscript><Superscript>2−</Superscript></Stack> in Antarctic calcitic evaporates

Nitrate radical NO₃²⁻ in calcitic evaporate was discovered in Antarctica. The distribution and formation of nitrate radical NO₃²⁻ in the calcite have been studied by pulse and continuous-wave electron spin resonance. In samples that had been annealed to destroy the NO₃²⁻, regeneration of the radical by γ-rays or UV light indicated that the radical was formed by UV light (with wavelengths less than 340 nm) from solar rays, not by environmental radiation. The nonuniform spatial distribution of the nitrate radical, which was deduced from high ratios of local spin density to total spin density, suggests that the nitrate impurity was introduced into the calcium carbonate after carbonate grain formation. Formation of the carbonate-containing nitrate requires the presence of high amounts of nitrate and a dry climate. Formation of the nitrate radical requires sample exposure to UV light. These conditions are satisfied in the environment of Antarctica.     

The dynamics of Rydberg electron wavepackets in NO

Rydberg electron wavepackets have been studied in molecular NO for a variety of rotational states of the ion core. Predominantly radial motion of the electron wavepacket is observed which is similar to that previously reported in atomic systems. Interference effects similar to those observed in unperturbed Rydberg series are evident and third and fourth order partial revivals are identified. Most interestingly, when the classical period of electronic motion is close to the classical period of rotation of the molecular ion, the molecular dynamics dominates the electronic dynamics.     

NMR diffusion spectroscopy as a measure of host-guest complex association constants and as a probe of complex size

The complexes of cyclohexylacetic acid and cholic acid with beta-cyclodextrin were studied by NMR diffusion coefficient measurements. The diffusion coefficient of the 1:1 cyclohexylacetic acid/beta-cyclodextrin complex, K(a) = 1800 +/- 100 M(-1), is slightly slower (3.23 +/- 0.07 x 10(-6) cm(2) s(-1)) than that of beta-cyclodextrin (3.29 +/- 0.07 x 10(-6) cm(2) s(-1)). The diffusion coefficient of the 1:1 cholic acid/beta-cyclodextrin complex, K(a) = 5900 +/- 800 M(-1), is significantly slower (2.93 +/- 0.07 x 10(-6) cm(2) s(-1)) than that of beta-cyclodextrin. The results indicate that caution should be exercised when studying host-guest complexation by the so-called 'single point' technique. A novel data treatment is introduced which takes into account the diffusion behavior of all of the species when determining K(a). Experimentally determined diffusion coefficients of complexes are also a useful probe of the size of host-guest complexes.     

A Rapid Freeze‐Quench Setup for Multi‐Frequency EPR Spectroscopy of Enzymatic Reactions

Electron paramagnetic resonance (EPR) spectroscopy in combination with the rapid freeze‐quench (RFQ) technique is a well‐established method to trap and characterize intermediates in chemical or enzymatic reactions at the millisecond or even shorter time scales. The method is particularly powerful for mechanistic studies of enzymatic reactions when combined with high‐frequency EPR (ν≥90 GHz), which permits the identification of substrate or protein radical intermediates by their electronic g values. In this work, we describe a new custom‐designed micro‐mix rapid freeze‐quench apparatus, for which reagent volumes for biological samples as small as 20 μL are required. The apparatus was implemented with homemade sample collectors appropriate for 9, 34, and 94 GHz EPR capillaries (4, 2, and 0.87 mm outer diameter, respectively) and the performance was evaluated. We demonstrate the application potential of the RFQ apparatus by following the enzymatic reaction of PpoA, a fungal dioxygenase producing hydro(pero)xylated fatty acids. The larger spectral resolution at 94 GHz allows the discernment of structural changes in the EPR spectra, which are not detectable in the same samples at the standard 9 GHz frequency.     

STUDIES OF ADHESION OF METAL PARTICLES TO SILICA PARTICLES BASED ON ZETA POTENTIAL MEASUREMENTS

The zeta-potentials of silica, copper, platinum and gold particles have been measured as a function of pH. The isoelectric points were found to be at pH 3.0, 5.8, 3.0 and 3.5, respectively. In the pH range 3.0 to 5.8 copper and silica particles are oppositely charged and accordingly the coating of silica with copper particles could be demonstrated. In the case of gold and platinum the sign of the charge is such that direct adhesion to silica particles cannot be expected and this was also demonstrated in the case of platinum.     

Synthesis and characterisation of sterically stabilised polypyrrole particles using a chemically reactive poly(vinyl amine)-based stabiliser

Poly(vinyl amine) (PVAm) was derivatised using 2-thiophenecarboxaldehyde via Michael addition to prepare a statistical copolymer stabiliser for the synthesis of primary amine-functionalised polypyrrole (PPy) particles. A minimum stabiliser concentration of around 20 % (w/v) relative to pyrrole was required for well-defined PPy particles of approximately 100–200 nm, as judged by transmission electron microscopy (TEM) and dynamic light scattering (DLS). FTIR spectroscopy confirmed that stabiliser grafting had occurred, while x-ray photoelectron spectroscopy (XPS) studies indicated a stabiliser surface coverage on the PPy particles of around 53 %. PPy particles prepared at stabiliser concentrations below 20 % (w/v) were not colloidally stable above pH 6. However, higher stabiliser concentrations (e.g., 50 % (w/v) based on pyrrole) led to a significant improvement, with colloidal stability being retained above pH 7. Long-term colloidal stability studies of PPy particles stored at pH 7.5 confirmed that the amine-based stabiliser produced more stable aqueous dispersions than the imine-based stabiliser, since the latter bond is hydrolytically unstable.     

Linear instability of planar shear banded flow

We study the linear stability of planar shear banded flow with respect to perturbations with wave vector in the plane of the banding interface, within the nonlocal Johnson-Segalman model. We find that perturbations grow in time, over a range of wave vectors, rendering the interface linearly unstable. Results for the unstable eigenfunction are used to discuss the nature of the instability. We also comment on the stability of phase separated domains to shear flow in model H.