Multi‐Colored Fibers by Self‐Assembly of DNA,Histone Proteins,and Cationic Conjugated Polymers

The development of biomolecular fiber materials with imaging ability has become more and more useful for biological applications. In this work, cationic conjugated polymers (CCPs) were used to construct inherent fluorescent microfibers with natural biological macromolecules (DNA and histone proteins) through the interfacial polyelectrolyte complexation (IPC) procedure. Isothermal titration microcalorimetry results show that the driving forces for fiber formation are electrostatic and hydrophobic interactions, as well as the release of counterions and bound water molecules. Color‐encoded IPC fibers were also obtained based on the co‐assembly of DNA, histone proteins, and blue‐, green‐, or red‐ (RGB‐) emissive CCPs by tuning the fluorescence resonance energy‐transfer among the CCPs at a single excitation wavelength. The fibers could encapsulate GFP‐coded Escherichia coli BL21, and the expression of GFP proteins was successfully regulated by the external environment of the fibers. These multi‐colored fibers show a great potential in biomedical applications, such as biosensor, delivery, and release of biological molecules and tissue engineering.     

Multi‐Colored Fibers by Self‐Assembly of DNA,Histone Proteins,and Cationic Conjugated Polymers

The development of biomolecular fiber materials with imaging ability has become more and more useful for biological applications. In this work, cationic conjugated polymers (CCPs) were used to construct inherent fluorescent microfibers with natural biological macromolecules (DNA and histone proteins) through the interfacial polyelectrolyte complexation (IPC) procedure. Isothermal titration microcalorimetry results show that the driving forces for fiber formation are electrostatic and hydrophobic interactions, as well as the release of counterions and bound water molecules. Color‐encoded IPC fibers were also obtained based on the co‐assembly of DNA, histone proteins, and blue‐, green‐, or red‐ (RGB‐) emissive CCPs by tuning the fluorescence resonance energy‐transfer among the CCPs at a single excitation wavelength. The fibers could encapsulate GFP‐coded Escherichia coli BL21, and the expression of GFP proteins was successfully regulated by the external environment of the fibers. These multi‐colored fibers show a great potential in biomedical applications, such as biosensor, delivery, and release of biological molecules and tissue engineering.     

Conjugated polymer nanoparticles as fluorescence switch for selective cell imaging

Fluorescence switch plays a vital role in bioelectronics and bioimaging.Herein,we presented a new kind of facile electrostatic complex nanoparticles(ECNs)for fluorescence switching in cells and marking of individual cell.The ECNs were prepared by mixing positively charged poly(6-(2-(thiophen-3-yl)ethoxy)hexyl trimethylammonium bromide)(PT)and negatively charged diarylethene sodium salt(DAECOONa).DAE-COONa is a photoswitchable molecule which can be transformed between the ring-closed fo rm and ring-open form under the irradiation of UV or visible light.The closed-form of DAE-COONa can efficie ntly quench the fluorescence of PT through intermolecular energy transfer,while the open form of DAE-COONa does not influence the emission of PT.Thus,the fluorescence of ECNs can be modulated by light irradiation,and the ECNs with good fluorescence switching performance have been employed for fluorescence imaging and individual cell lighting up process successfully.We demonstrate that the electrostatic complex strategy provides a facile method to construct fluorescence switch fo r selective cell marking and imaging applications.     

pH Dependence of Surface Enhanced FT-Raman Scattering of Ω-Mercaptoalkanols on Silver Surface

Fourier transform surface enhanced Raman spectrometer is used to characterize Ω-mercaptoalkanols (HO(CH₂)_nSH, n=2, 6) chemisorption on silver surface from their aqueous solution of different pH values.     

Effects of temperature and dissolved CO<Subscript>2</Subscript> on the scaling of water in the presence of copper and zinc

In this study, through the method of rapid controlled precipitation (RCP), the influence of temperature and dissolved CO₂ on the scaling capacity of mineral water in the presence of copper and zinc ions was studied in laboratory experiments. The results indicated that with a rise in temperature or concentration of dissolved CO₂, the scaling time of Salvetat water greatly decreased and the rate of precipitation considerably increased; therefore, the minimum dosage of copper or zinc ion for achieving total inhibition of scaling needed to be increased, which would provide better guidance for practical use of metal ions in inhibiting the scaling of drinking water.     

分光路二维剪切干涉强激光腔镜变形检测系统

介绍一种分光路二维剪切干涉光电检测系统。该系统实现了两正交方向(二维)同时进行检测与计算机自动化数据处理;采用了一系列消噪声措施和两图像相减等技术,使得波前重构中能较好地采用简单的数值积分方法;并得到了在士10μm的测量范围内,方差不超过0. 257μm和0. 05μm的灵敏度。利用该系统成功地对强激光腔镜变形过程实现了动态检测。     

化学氧碘激光器光腔中碘分子浓度的测量

依据Ｂｅｅｒ定律，采用双光路法，利用Ａｒ￣＋激光器的４８８ｎｍ线作为探测激光，对化学氧碘激光器光腔中的碘分子浓度进行了瞬时测量，详细地研究了化学氧碘激光器重要部件之一，激活介质的给体－腆池的工作性能对其他工作参数的依赖关系，并估计了ｋｗ级化学氧碘激光器的最佳氧碘配比约为９０：１．     

Selective biocompatibility and responsive imaging property of cationic conjugated polyelectrolyte to cancer cells

Herein, we bring novel insights into a water-soluble conjugated polyelectlyte by deeply studying its properties in cells and observe fl uorescence enhancement of blue emission and turn-on of long-wavelength emission of PFP in HepG2 cells.     

Thioether-functionalized mesoporous fiber membranes: sol-gel combined electrospun fabrication and their applications for Hg2+ removal

Mesoporous polyvinylpyrrolidone (PVP)/SiO(2) composite nanofiber membranes functionalized with thioether groups have been fabricated by a combination method of sol-gel process and electrospinning. The precursor sol was synthesized by one-step co-condensation of tetraethyl orthosilicate (TEOS) and 1,4-bis(triethoxysilyl)propane tetrasulfide (BTESPTS, (CH(3)CH(2)O)(3)Si(CH(2))(3)S-S-S-S(CH(2))(3)Si-(OCH(2)CH(3))(3)), with the triblock copolymer poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (P123, EO(20)PO(70)EO(20)) as template. After the addition of PVP, nanofiber membranes were prepared by electrospinning. The membranes were characterized by scanning electron microscopy (SEM), high-resolution transmission electron microscopy (HRTEM) images, X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), N(2) adsorption-desorption isotherms, and an Elementar Vario EL analyzer. The composites were used as highly selective adsorbents for Hg(2+) due to the modification with thioether groups (-S-), and were conveniently separated from the waste water. The composite could be regenerated through acidification.