排序方式: 共有19条查询结果,搜索用时 15 毫秒
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Bharathi Avula Babu L. Tekwani Narayan D. Chaurasiya NP Dhammika Nanayakkara Yan‐Hong Wang Shabana I. Khan Vijender R. Adelli Rajnish Sahu Mahmoud A. Elsohly James D. McChesney Ikhlas A. Khan Larry A. Walker 《Journal of mass spectrometry : JMS》2013,48(2):276-285
Therapeutic efficiency and hemolytic toxicity of primaquine (PQ), the only drug available for radical cure of relapsing vivax malaria are believed to be mediated by its metabolites. However, identification of these metabolites has remained a major challenge apparently due to low quantities and their reactive nature. Drug candidates labeled with stable isotopes afford convenient tools for tracking drug‐derived metabolites in complex matrices by liquid chromatography‐tandem mass spectrometry (LC‐MS‐MS) and filtering for masses with twin peaks attributable to the label. This study was undertaken to identify metabolites of PQ from an in vitro incubation of a 1:1 w/w mixture of 13C6‐PQ/PQ with primary human hepatocytes. Acquity ultra‐performance LC (UHPLC) was integrated with QTOF‐MS to combine the efficiency of separation with high sensitivity, selectivity of detection and accurate mass determination. UHPLC retention time, twin mass peaks with difference of 6 (originating from 13C6‐PQ/PQ), and MS‐MS fragmentation pattern were used for phenotyping. Besides carboxy‐PQ (cPQ), formed by oxidative deamination of PQ to an aldehyde and subsequent oxidation, several other metabolites were identified: including PQ alcohol, predictably generated by oxidative deamination of PQ to an aldehyde and subsequent reduction, its acetate and the alcohol's glucuronide conjugate. Trace amounts of quinone‐imine metabolites of PQ and cPQ were also detected which may be generated by hydroxylation of the PQ/cPQ quinoline ring at the 5‐position and subsequent oxidation. These findings shed additional light on the human hepatic metabolism of PQ, and the method can be applied for identification of reactive PQ metabolites generated in vivo in preclinical and clinical studies. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
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E. Barnitzke D. Brunton J. A. Scherrer G. E. F. Lundell H. Gehle E. Hildebrandt und E. Schiller 《Fresenius' Journal of Analytical Chemistry》1933,94(7-8):257-259
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Acta Mechanica Sinica - This paper provides a short overview of how to use machine learning to build data-driven models in fluid mechanics. The process of machine learning is broken down into five... 相似文献
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Joshua L. Proctor Steven L. Brunton J. Nathan Kutz 《The European physical journal. Special topics》2016,225(13-14):2413-2434
The increasing ubiquity of complex systems that require control is a challenge for existing methodologies in characterization and controller design when the system is high-dimensional, nonlinear, and without physics-based governing equations. We review standard model reduction techniques such as Proper Orthogonal Decomposition (POD) with Galerkin projection and Balanced POD (BPOD). Further, we discuss the link between these equation-based methods and recently developed equation-free methods such as the Dynamic Mode Decomposition and Koopman operator theory. These data-driven methods can mitigate the challenge of not having a well-characterized set of governing equations. We illustrate that this equation-free approach that is being applied to measurement data from complex systems can be extended to include inputs and control. Three specific research examples are presented that extend current equation-free architectures toward the characterization and control of complex systems. These examples motivate a potentially revolutionary shift in the characterization of complex systems and subsequent design of objective-based controllers for data-driven models. 相似文献
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Glenzer SH MacGowan BJ Meezan NB Adams PA Alfonso JB Alger ET Alherz Z Alvarez LF Alvarez SS Amick PV Andersson KS Andrews SD Antonini GJ Arnold PA Atkinson DP Auyang L Azevedo SG Balaoing BN Baltz JA Barbosa F Bardsley GW Barker DA Barnes AI Baron A Beeler RG Beeman BV Belk LR Bell JC Bell PM Berger RL Bergonia MA Bernardez LJ Berzins LV Bettenhausen RC Bezerides L Bhandarkar SD Bishop CL Bond EJ Bopp DR Borgman JA Bower JR Bowers GA Bowers MW Boyle DT Bradley DK Bragg JL Braucht J 《Physical review letters》2011,106(8):085004
We demonstrate the hohlraum radiation temperature and symmetry required for ignition-scale inertial confinement fusion capsule implosions. Cryogenic gas-filled hohlraums with 2.2 mm-diameter capsules are heated with unprecedented laser energies of 1.2 MJ delivered by 192 ultraviolet laser beams on the National Ignition Facility. Laser backscatter measurements show that these hohlraums absorb 87% to 91% of the incident laser power resulting in peak radiation temperatures of T(RAD)=300 eV and a symmetric implosion to a 100 μm diameter hot core. 相似文献
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It has been shown by ESR spectroscopy that the title reaction involves abstraction of hydrogen from the phosphite, since at ?10°C the reaction has a kinetic deuterium isotope effect, kH/kD, or ~3. The rate constant for hydrogen abstraction is c. 2 × 104 M?1 s?1. There is no significant addition of alkoxyl radicals to the phosphite. 相似文献
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