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KL Britton HF Rogers Y Asano T Dairi Y Kato TJ Stillman DW Rice 《Acta crystallographica. Section D, Biological crystallography》1998,53(4):124-126
The novel NAD+-linked opine dehydrogenase from a soil isolate Arthrobacter sp. strain 1C belongs to an enzyme superfamily whose members exhibit quite diverse substrate specificites. Crystals of this opine dehydrogenase, obtained in the presence or absence of co-factor and substrates, have been shown to diffract to beyond 1.8 ? resolution. X-ray precession photographs have established that the crystals belong to space group P21212, with cell parameters a = 104.9, b = 80.0, c = 45.5 ? and a single subunit in the asymmetric unit. The elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this novel class of dehydrogenases to enable a comparison to be made with other enzyme families and also as the basis for mutagenesis experiments directed towards the production of natural and synthetic opine-type compounds containing two chiral centres. 相似文献
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T. J. Bonagamba F. Becker‐Guedes E. R. DeAzevedo K. Schmidt‐Rohr 《Journal of Polymer Science.Polymer Physics》2001,39(20):2444-2453
Slow side‐group dynamics in a series of five poly(alkyl methacrylate)s with various side‐group sizes [poly(methacrylic acid) (PMAA), poly(methyl methacrylate) (PMMA), poly(ethyl methacrylate) (PEMA), poly(isobutyl methacrylate) (PiBMA), and poly(cyclohexyl methacrylate), with ? H, ? CH3, ? CH2CH3, ? CH2CH(CH3)2, and ? cyclohexyl alkyl substituents (CODEX), respectively] were studied quantitatively by centerband‐only detection of exchange nuclear magnetic resonance (NMR). Flips and small‐angle motions of the ester groups associated with the β relaxation were observed distinctly in the CODEX NMR data, and the fraction of slowly flipping groups was measured with a precision of 3%. In PMMA, 34% of the side groups flipped on a 1‐s timescale, whereas the fraction was 31% in PEMA at 25 °C. Even the large isobutylether and cyclohexylester side groups flipped in the glassy state, although the flipping fraction was reduced to 22 and about 10%, respectively. In PMAA, no slow side‐group flips were detected on the 1‐s timescale. A striking difference in the temperature dependence of the flipping fraction in PMMA versus PEMA and PiBMA was observed. In PMMA, the flipping fraction was temperature‐independent between 25 and 80 °C, whereas in PEMA, it increased continuously from 31 to 60% between 25 and 60 °C. A similar doubling was also observed in PiBMA. © 2001 John Wiley & Sons, Inc. J Polym Sci Part B: Polym Phys 39: 2444–2453, 2001 相似文献
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Karim Dahmouche Mohamed Atik Nilson C. Mello Tito J. Bonagamba Horacio Panepucci Michel A. Aegerter Patrick Judeinstein 《Journal of Sol-Gel Science and Technology》1997,8(1-3):711-715
Two families of hybrid organic-inorganic composites exhibiting Li+ ionic conduction (ORMOLYTES) have been prepared by the sol-gel process. The first family, prepared from a mixture of 3-isocyanatopropyltriethoxysilane, O,O Bis (2-aminopropyl)-polyethyleneglycol (or O,O Bis (2-aminopropyl)-polypropyleneglycol) and lithium salt, presents chemical bonds between the organic and the inorganic phase and an ionic conductivity higher than 10-4 S m-1 at room temperature. Their properties have been related to their structure using liquid state NMR measurements of 7Li between -100°C and +100°C and the DMTA technique. In the second family, prepared by ultrasonic method from a mixture of tetraethoxysilane (TEOS), polyethyleneglycol (PEG) and lithium salt, the organic and inorganic phases are not chemically bonded. Ionic conductivity has been studied as a function of the polymer chain length and concentration. Values of up to 10-2 S m-1 at room temperature have been obtained (with a silica-PEG300 system, PEG/TEOS = 40% in weight). Again, structure was investigated by liquid state 7Li NMR measurements. 相似文献
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deAzevedo ER Tozoni JR Schmidt-Rohr K Bonagamba TJ 《The Journal of chemical physics》2005,122(15):154506
One-dimensional (1D) exchange NMR experiments can elucidate the geometry, time scale, memory, and heterogeneity of slow molecular motions (1 ms-1 s) in solids. The one-dimensional version of pure-exchange (PUREX) solid-state exchange NMR, which is applied to static samples and uses the chemical shift anisotropy as a probe for molecular motion, is particularly promising and convenient in applications where site resolution is not a problem, i.e., in systems with few chemical sites. In this work, some important aspects of the 1D PUREX experiment applied to systems with complex molecular motions are analyzed. The influence of intermediate-regime (10 micros-1 ms) motions and of the distribution of reorientation angles on the pure-exchange intensity are discussed, together with a simple method for estimating the activation energy of motions occurring with a single correlation time. In addition, it is demonstrated that detailed information on the motional geometry can be obtained from 1D PUREX spectral line shapes. Experiments on a molecular crystal, dimethyl sulfone, confirm the analysis quantitatively. In two amorphous polymers, atactic polypropylene (aPP) and polyisobutylene (PIB), which differ only by one methyl group in the repeat unit, the height of the normalized exchange intensity clearly reveals a striking difference in the width of the distribution of correlation times slightly above the glass transition. The aPP shows the broad distribution and Williams-Landel-Ferry temperature dependence of correlation times typical of polymers and other "fragile" glass formers. In contrast, the dynamics in PIB occur essentially with a single correlation time and exhibits Arrhenius behavior, which is more typical of "strong" glass formers; this is somewhat surprising given the weak intermolecular forces in PIB. 相似文献
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K. Dahmouche P.H. De Souza T.J. Bonagamba H. Paneppucci P. Judeinstein S.H. Pulcinelli C.V. Santilli 《Journal of Sol-Gel Science and Technology》1998,13(1-3):909-913
Two groups of hybrid organic-inorganic composites exhibiting ionic conduction properties, so called ORMOLYTES (organically modified electrolytes), have been prepared by the sol-gel process. The first group has been prepared from mixture of a lithium salt and 3-isocyanatopropyltriethoxysilane(IsoTrEOS),O,O-bis(2-aminopropyl)polypropyleneglycol. These materials produce chemical bonds between the organic (polymer) and the inorganic (silica) phases. The second group has been prepared by an ultrasonic method from a mixture of tetraethoxysilane (TEOS), polypropyleneglycol and a lithium salt. The organic and inorganic phases are not chemically bonded in these samples. The Li+ ionic conductivity, , of all these materials has been studied by AC impedance spectroscopy up to 100°C. Values of up to 10–6 –1 · cm–1 have been found at room temperature. A systematic study of the effects of lithium concentration, polymer chain length and the polymer to silica weight ratio on shows that there is a strong dependence of on the preparation conditions. The dynamic properties of the Li+ ion and the polymer chains as a function of temperature between –100 and 120°C were studied using 7Li solid-state NMR measurements. The ionic conductivity of both families are compared and particular attention is paid to the nature of the bonds between the organic and inorganic components. 相似文献
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Bonk FA deAzevedo ER Sarthour RS Bulnes JD Freitas JC Guimarães AP Oliveira IS Bonagamba TJ 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2005,175(2):226-234
This article presents the realization of many self-reversible quantum logic gates using two-qubit quadrupolar spin 3/2 systems. Such operations are theoretically described using propagation matrices for the RF pulses that include the effect of the quadrupolar evolution during the pulses. Experimental demonstrations are performed using a generalized form of the recently developed method for quantum state tomography in spin 3/2 systems. By doing so, the possibility of controlling relative phases of superimposed pseudo-pure states is demonstrated. In addition, many aspects of the effect of the quadrupolar evolution, occurring during the RF pulses, on the quantum operations performance are discussed. Most of the procedures presented can be easily adapted to describe selective pulses of higher spin systems (>3/2) and for spin 1/2 under J couplings. 相似文献
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Tozoni JR Teles J Auccaise R Oliveira-Silva R Rivera-Ascona C Vidoto EL Guimarães AP Oliveira IS Bonagamba TJ 《Journal of magnetic resonance (San Diego, Calif. : 1997)》2011,212(2):265-273
In this paper we present a series of high-resolution zero-field NMR spectra of the polycrystalline intermetallic compound GdAl2. The spectra were obtained with the sample at 4.2 K in the ordered magnetic state and in the absence of an external static magnetic field. Using a sequence composed of two RF pulses, we obtained up to five multi-quantum echoes for the 27Al nuclei, which were used to construct the zero-field NMR spectra. The spectra obtained from the FID observed after the second pulse and the even echoes exhibited higher resolution than the odd ones. In order to explain such behavior, we propose a model in which there are two regions inside the sample with different inhomogeneous spectral-line broadenings. Moreover, with the enhanced resolution from the FID signal, we were able to determine quadrupolar couplings with great precision directly from the respective spectra. These results were compared with those obtained from the quadrupolar oscillations of the echo signals, and showed good agreement. Similar data were also obtained from 155Gd and 157Gd nuclei. 相似文献
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