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1.
A method using capillary gel electrophoresis with laser-induced fluorescence detection is described which permits complete sequence determination of antisense DNA analogues of unknown sequence. This method, originally created as a tool to confirm the sequence of antisense oligonucleotides being developed as therapeutic drugs, utilizes data collected under a range of experimental conditions described by the Ogston model as applied to gel electrophoresis. A linear relationship independent of experimental conditions between the relative electrophoretic migration time and the oligonucleotide base number was observed and is shown to be consistent with a simplified version of this model and can be used to facilitate the sequence determination. 相似文献
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Marcelino Maneiro Manuel R. Bermejo M. Isabel Fernndez Ana M. Gonzlez‐Noya Alexei M. Tyryshkin Robin G. Pritchard 《无机化学与普通化学杂志》2003,629(2):285-290
A new MnIII‐Schiff base complex, [MnL(OH2)](ClO4) ( 1 ) (H2L = N, N′‐bis‐(3‐Br‐5‐Cl‐salicylidene)‐1, 2‐diimino‐2‐methylethane), an inorganic model of the catalytic center (OEC, Oxygen Evolving Complex) in photosystem II (PSII), has been synthesized and characterized by elemental analysis, IR and EPR spectroscopy, mass spectrometry, magnetic susceptibility measurement and the study of its redox properties by cyclic and normal pulse voltammetry. This complex mimics reactivity (showing a relevant photolytic activity), and also some structural characteristics (parallel‐mode MnIII EPR signal from partially assembled OEC cluster) of the natural OEC. The complex 1 was found to rearrange in solution into a crystallographically solved square‐pyramidal complex, [MnLL′] ( 2 ) (HL′ = 6‐bromo‐4‐chloro‐2‐cyanophenol), through a process, which probably liberates radical species (detected by EPR), and provokes a C—N bond cleavage in the ligand. A photo‐radical mechanism is discussed to explain this rearrangement. 相似文献
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V. F. Dmitriev 《Technical Physics》2002,47(8):1024-1031
A synthesis method for ladder-type filters based on SAW resonators is described. SAW devices are designed with modified equations for coupled waves. Nomograms for choosing the geometry of the filters on ST, X-cut quartz and on 36° Y, X-cut LiTaO3 substrates are presented. Theoretical results are compared with experimental data. 相似文献
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Florin Radu Alexei Nefedov Johannes GrabisGregor Nowak Andre BergmannHartmut Zabel 《Journal of magnetism and magnetic materials》2006
We have used soft X-ray resonant magnetic scattering (XRMS) to search for the presence of an effective ferromagnetic moment belonging to the antiferromagnetic (AF) layer which is in close contact with a ferromagnetic (F) layer. Taking advantage of the element specificity of the XRMS technique, we have measured hysteresis loops of both Fe and CoO layers of a CoO(40 Å)/Fe (150 Å) exchange bias bilayer. From these measurements we have concluded that the proximity of the F layer induces a magnetic moment in the AF layer. The F moment of the AF layer has two components: one is frozen and does not follow the applied magnetic field and the other one follows in phase the ferromagnetic magnetization of the F layer. The temperature dependence of the F components belonging to the AF layer is shown and discussed. 相似文献
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V. P. Koshelets S. V. Shitov L. V. Filippenko P. N. Dmitriev A. N. Ermakov A. S. Sobolev M. Yu. Torgashin 《Radiophysics and Quantum Electronics》2003,46(8-9):618-630
We overview recent achievements in the field of cryogenic submillimeter-wave receivers based on superconductor–insulator–superconductor (SIS) tunnel junctions. The main attention is paid to the novel superconducting integrated receivers (SIRs) with an on-chip superconducting local oscillator. The single-chip microcircuit of the receiver, which integrates a quantum mixer based on the nonlinearity of a quasi-particle current in the SIS junction, a planar superconducting receiving antenna, and a cryogenic local oscillator, is described. Being dc-powered only by batteries, such a microcircuit operates as a submillimeter-wave superheterodyne receiver without any additional microwave equipment. Such receivers are very attractive for radioastronomical research, space communication systems, and monitoring of the environment from satellites, balloons, and special aircraft. A breadboard of a superconducting spectrometer with a phase-locked flux-flow oscillator (FFO) has been developed and tested. A frequency resolution better than 10 kHz was reached at a frequency of 365 GHz. We describe a balloon-borne 500-650 GHz integrated spectrometer for oblique atmospheric sounding, developed for the international Terahertz Limb Sounder (TELIS) project. The first flight is scheduled for 2005. 相似文献
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