Design and preparation of filter sets for fourplex fluorescence PCR instruments

Aiming at the crosstalk problem caused by small spectral intervals between fluorescent reagents in fourplex fluorescence quantitative polymerase chain reaction （PCR） detection analysis system, we calculate and analyse the effect of cut-off steepness, central wavelength positioning, and bandwidth of filters on crosstalk. Design and prepare four sets of fluorescence excitation and emission filters with proper shape cut-off steepness （optical density （OD） from OD 0.3 to OD 6 is less than 8 nm） and bandwidth （9-11 nm）. In fourDlex PCR instrument, crosstalk coefficient in all four channels are less than 0.3%.     

数字电影反光镜3D扫描面形检测与评价

通过3D扫描的方法,对椭球反光镜的面形进行了检测,并与标准椭球面形进行最佳拟合对比,以E210反光镜为例。结果表明:3D扫描能够完成椭球反光镜的检测,E210反光镜在底孔部位存在加工过量,在端口部位存在近似十字形对称的加工不足的现象,加工误差范围为-0.06 mm ~+0.08 mm,符合轮廓度误差0.1 mm的要求。3D扫描点云能够进行逆向反求并建立实际反射面模型,进一步模拟反光镜的反射光斑形状、大小等参数,模拟结果与检测结果一致。