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将核酸外切酶Ⅲ诱导的双重信号放大技术与MoS2纳米片的荧光猝灭性质结合,构建了一种高灵敏高选择性的DNA检测方法.首先设计两条末端修饰荧光基团的探针核酸(HP1和HP2).由于两条探针核酸具有3'粘性末端,使其不会被核酸外切酶Ⅲ降解,因而被吸附于MoS2纳米片而猝灭其荧光.当目标DNA存在时,会促使核酸外切酶Ⅲ启动双重信号放大反应,并将探针核酸降解成大量的不能吸附于MoS2纳米片表面的荧光碎片.在优化条件下,目标DNA浓度在0.5~6.0 pmol/L范围内与荧光信号变化呈良好的线性关系,检出限为0.28 pmol/L.与单重信号放大技术相比,本方法极大改善了分析灵敏度和检出限,且具有良好的单碱基错配区分能力. 相似文献
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In this paper, we consider multi-agent consensus problems
in a decentralised fashion. The interconnection topology graph among
the agents is switching and undirected. The agent dynamics is
expressed in the form of a double integrator model. Two different
cases are considered in this study. One is the leader-following case
and the other is leaderless case. Based on graph theory and common
Lyapunov function method, some sufficient conditions are obtained
for the consensus stability of the considered systems with the
neighbour-based feedback laws in both leader-following case and
leaderless case respectively. Finally, two numerical examples are
given to illustrate the obtained results. 相似文献
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