A new visual investigation into nanogold-based genechip assay by atomic force and scanning tunneling microscope

Conformations of surface atoms in various stages of nanogold-based genechip testing are scanned by the atomic force and scanning tunneling microscope. We intuitively observe the process and differences in probe combination, nucleic acid hybridization, and silver staining, which might be useful to validate the assay method of genechip. We hope to use this technology to make the other invisible chemical or biochemical reaction become visible and convincible in the future.     

人工合成杆状病毒后期启动子的探讨

本文以苜蓿丫纹夜蛾核型多角体病毒(Autographa californica Nuclear PolyhedrosisVirus,AcNPV)多角体、p10、病毒粒子衣壳和核心碱性蛋白启动子的保守序列为基础,设计合成杆状病毒后期启动子——92和96碱基的互补寡聚核苷酸,并以此重组出5株在多角体启动序列、翻译起始密码子ATG上游-92处,分别或同时插入反方向的合成和多角体启动子,以及氯霉素乙酰基转移酶(CAT)基因的AcNPV毒株,探讨了带这些启动子的CAT在草地夜蛾(Spodoptera frugiperda)细胞中的表达规律及多角体基因对其表达的影响。形成多角体的重组病毒株AcNPV-vⅪⅤⅥ~+CAT和AcNPV-vSⅪⅤⅥ~+CAT中,处于合成启动子与多角体ⅪⅤ启动顺序下游的CAT基因在细胞中的表达量,要比只在多角体启动子带动下高3倍。