干细胞迁移机理的近场扫描光学显微术研究

将内皮细胞生长因子(VEGF)置于甲基纤维素碟中形成VEGF的浓度梯度分布,并将人脐带间充质干细胞(Mesenchymal stem cells,MSCs)于此浓度梯度中培养,观察VEGF能否诱导MSCs定向迁移。应用近场扫描光学显微术(Near-field scanning optical microscopy,NSOM)同时获取了VEGF诱导前后的MSCs的形貌和光学信息。结果表明,近场光学图观测到形貌图上所没有的黑色斑点,分析认为这些黑斑为细胞的黏着斑。近场光学图显示经过VEGF诱导后细胞的黏着斑数量明显增加。同时,对诱导前后干细胞的骨架蛋白进行免疫荧光标记并用共聚焦显微镜进行观察,结果表明细胞骨架由诱导前的无序状态转变为诱导后的有序状态,说明诱导后的干细胞处于迁移状态。光学超微结构图则显示了诱导后干细胞表面的光学细节比诱导前细胞大量增加,出现了大量直径约200 nm的光斑,这是由于细胞表面大量分泌黏附分子等蛋白分子引起的,这些结果为VEGF能够诱导MSCs进行定向迁移提供了实验依据和可视化证明。也表明NSOM不但能提供高分辨的光学分辨率,还可提供生物细胞精细结构的更深层次的光学信息。     

统计物理教学中引入Jarzynski等式的必要性

20世纪90年代以来,统计物理学界最重要的成果之一是Jarzynski在1997年得到的一个远离平衡态等式,称为Jarzynski等式。其具有广泛的适用性,且已经在单个DNA分子的实验上得到了证实和应用。Jarzynski等式具有重要的意义:利用该等式可以由非平衡物理过程中功的测量来精确地计算两个平衡态之间Helmholz自由能的差。本文简要综述了Jarzynski等式理论和实验的重要研究成果,表明Jarzynski等式是物理世界中一个非常本质的规律,进而阐明统计物理教材或教学的讨论课程中引入这一等式的必要性。     

Ballistic diffusion induced by non-Gaussian noise

In this letter,we have analyzed the diffusive behavior of a Brownian particle subject to both internal Gaussian thermal and external non-Gaussian noise sources.We discuss two time correlation functions C(t) of the non-Gaussian stochastic process,and find that they depend on the parameter q,indicating the departure of the non-Gaussian noise from Gaussian behavior:for q ≤ 1,C(t) is fitted very well by the first-order exponentially decaying curve and approaches zero in the longtime limit,whereas for q 1,C(t) can be approximated by a second-order exponentially decaying function and converges to a non-zero constant.Due to the properties of C(t),the particle exhibits a normal diffusion for q ≤ 1,while for q 1 the non-Gaussian noise induces a ballistic diffusion,i.e.,the long-time mean square displacement of the free particle reads [x(t)-x(t)]2∝t2.     

Nonergodic Brownian Motion

The long-time behavior of a system is suggested to confirm nonergodicity of non-Markovian Brownian dynamics, namely, whether the stationary probability density function （PDF） of the system characterized mainly by low moments of variables depends on the initial preparation. Thus we classify nonergodic Brownian motion into two classes： the class-I is that the PDF of a force-free particle depends on the initial velocity and the equilibration can be recovered through a bounded potential; while the PDF in the class-H depends on the initial coordinate and the equilibration can not be approached by introducing any potential. We also compare our result with the conditions of three kinds for ergodicity.     

周期场中非各态历经布朗运动

将自由状态下呈弹道扩散的非各态历经系统置于周期场中,进而将非各态历经布朗运动分为两类.第一类是阻尼核的Laplace变换的低频为零的系统,当温度远大于势垒高度时,系统平均能量的动能部分依赖粒子的初始速度分布;随温度降低,系统的各态历经性得到恢复.然后将第一类系统的稳定速度变量作为一个内部噪声,再去驱动一个自由布朗粒子,则阻尼核的Laplace变换在零频时为无穷大.结果发现,粒子扩散系数随温度的增加而趋于零,显示一种经典局域化特征,系统的渐进分布依赖于初始坐标分布.这是第二类非各态历经性运动,不能通过外加势而恢复. 关键词： 非各态历经 非Markov布朗运动 扩散系数 噪声谱     

Energy Transfer in the Light-Harvesting Complexes of Purple Bacteria

In this paper, we use a nonlinear decohering quantum model to study the initial step of photosynthesis which is an ultrafast transfer process of absorption the sunlight by light-harvesting complexes and electronic excitation transfer to the reaction center(RC). In this decohering model, the Hamiltonian of the system commutes with the systemenvironment interaction. We take B850 ring of light-harvesting complex II(LH-II) in purple bacteria as an example to calculate the efficiency of the energy transfer as a function of time. We find that the environmental noise can make the LH-II have stable energy transfer efficiency over a long time. This is to say that the environmental noise which is the decohering source has advantage of the energy transfer in the process of photosynthesis.     

外部信号驱动下胶原粒子在谐振势场中的协作效应

基于朗之万方程研究了一个运动在简谐势场中胶原粒子的热力学行为,给出了其在周期信号驱动下的有效温度和耗散功的解析表达式.结果表明：外部周期搅拌能够聚合环境热能传递给胶原粒子,使其有效温度大幅度增加,这是势场、外部信号及噪声的协作机理所引起;但是在共振条件下,外界所作的耗散功也达到极大. 关键词： 耗散功 有效温度 周期信号 协作效应