脂质体电动色谱法评价阿魏酸与生物膜的相互作用

利用脂质体与生物膜结构的相似性,将脂质体加入毛细管电泳缓冲溶液中作为假固定相,在数分钟内测定了阿魏酸的脂水分配系数Klw,建立了脂质体电动色谱评价阿魏酸与生物膜相互作用的方法。研究了脂质体中胆固醇的含量、缓冲溶液pH值和缓冲体系对Klw的影响。结果表明,在实验条件范围内(胆固醇含量0~30%,pH值4.0~12.0),胆固醇含量升高,缓冲溶液pH值增大,Klw降低;在不同的缓冲体系中,离子强度越大,Klw越大。脂水分配系数的变化反映了阿魏酸与生物膜相互作用。     

Effect of Nitridation on Morphology, Structural Properties and Stress of AlN Films

We investigate effects of nitridation on AlN morphology, structural properties and stress. It is found that 3min nitridation can prominently improve AlN crystal structure, and slightly smooth the surface morphology. However, 10min nitridation degrades out-of-plane crystal structure and surface morphology instead. Additionally, 3-min nitridation introduces more tensile stress （1.5 GPa） in AlN films, which can be attributed to the weaker islands 2D coalescent. Nitridation for lOmin can introduce more defects, or even forms polycrystallinity interlayer, which relaxes the stress. Thus, the stress in AlN with 10 min nitridation decreases to -0.2 GPa compressive stress.     

组合手性萃取拆分克伦特罗外消旋体

以D-二苯甲酰酒石酸(D-DBTA)和D-二对甲苯甲酰酒石酸(D-DTTA)的组合作为手性选择剂,研究克伦特罗对映体在水相和有机相中的萃取分配行为,优化了手性萃取条件,考察了组合手性选择剂的不同摩尔浓度比、有机溶剂、水相pH值和亲脂性阴离子BPh4-对手性萃取性能的影响,并测定了手性萃取拆分过程中的热力学函数.在优化的手性萃取条件下,有机相中的对映体过量值(e.e.%)可大于10%,而所使用的选择剂量相对大为降低.热力学数据分析表明,该手性萃取过程为焓控过程.     

A Comparison between AIN Films Grown by MOCVD Using Dimethylethylamine Alane and Trimethylaluminium as the Aluminium Precursors

Aluminium nitride （AIN） films grown with dimethylethylamine Mane （DMEAA） are compared with the ones grown with trimethylaluminium （TMA）. In the high-resolution x-ray diffraction Ω scans, the full width at half maximum （FWHM） of （0002） AIN films grown with DMEAA is about 0.70 deg, while the FWHM of （0002） AIN films grown with TMA is only 0.11 deg. The surface morphologies of the films are different, and the rms roughnesses of the surface are approximately identical. The rms roughness of AIN films grown with DMEAA is 47.4nm, and grown with TMA is 69.4nm. Although using DMEAA as the aluminium precursor cannot improve the AIN crystal quality, AIN growth can be reached at low temperature of 673 K. Thus, DMEAA is an alternative aluminium precursor to deposit AIN film at low growth temperatures.     

Influence of Different Interlayers on Growth Mode and Properties of InN by MOVPE

We grow InN epilayers on different interlayers by metal organic vapour phase epitaxy （MOVPE） method, and investigate the effect of interlayer on the properties and growth mode of InN films. Three InN samples were deposited on nitrided sapphire, low-temperature InN （LT-InN） and high-temperature GaN （HT-GaN）, respectively. The InN layer grown directly on nitrided sapphire owns the narrowest x-ray diffraction rocking curve （XRC） width of 300 aresee among the three samples, and demonstrates a two-dimensional （2D） step-flow-like lateral growth mode, which is much different from the three-dimensional （3D） pillar-like growth mode of LT-InN and HT-GaN buffered samples. It seems that mismatch tensile strain is helpful for the lateral epitaxy of InN film, whereas compressive strain promotes the vertical growth of InN films.     

ZnO Nanostructures Grown on A1N/Sapphire Substrates by MOCVD

ZnO nanorods and nanotubes are successful synthesized on A1N/sapphire substrates by metal-organic chemical vapour deposition （MOGVD）. The different morphology and structure properties of ZnO nanorods and nanotubes are found to be affected by the A1N under-layer. The photoluminescence spectra show the optical properties of the ZnO nanorods and nanotubes, in which a blueshift of UV emission is observed and is attributed to the surface effect.[第一段]     

厚体液膜动力学拆分普萘洛尔外消旋体

以S,S-酒石酸正十二酯和H3BO生成的配合物为手性载体, 通过量热分析结果初步证明该配合物的存在, 采用厚体液膜拆分了普萘洛尔外消旋体. 考察了S,S-酒石酸十二酯浓度和水相pH值对拆分性能的影响, 优化了其拆分操作条件, 同时进行了动力学分析. 得出厚体液膜拆分的一般规律, 即其拆分属于动力学拆分过程, 适当提高手性载体的浓度有利于提高对映单体流量和拆分的立体选择性, 水相pH值对于拆分性能有着重要的影响. 其拆分过程中萃取反应过程为控速步骤, 对映单体的跨膜传递可以用两个串联的准一级不可逆过程进行描述.     

福林酚分光光度法测定蜂毒中总蛋白质

建立福林酚分光光度法测定蜂毒中总蛋白含量的方法。参照《中国药典》2020年版通则0731蛋白质含量测定方法第二法,以水作为溶剂,在样品溶液中加入福林酚溶液4.00 mL,于25 ℃下反应40 min,在750 nm波长处测定吸光度,标准曲线法定量。总蛋白质的质量浓度(x)在44.4～222.0 μg/mL范围内与吸光度(y)线性关系良好,线性方程为y=3.078 8x+0.025 7,相关系数为0.999 0,定量限为4.6 μg/mL。总蛋白质测定结果的相对标准偏差为1.6%(n=6),样品加标回收率为96.56%～104.53%。该方法可用于蜂毒样品中总蛋白含量的测定。     

厚体液膜拆分克伦特罗外消旋体及其动力学研究

以D-( )-二苯甲酰酒石酸(D-( )-DBTA)为流动载体,研究了克伦特罗(clenbuterol,Cle)对映体的厚体液膜法拆分,建立了手性拆分条件和动力学拆分模型。考察了D-( )-DBTA浓度、缓冲液pH值对手性拆分性能的影响,进行了动力学分析,并测定了膜-料液界面的萃取反应表观速率常数k1和膜-反萃相界面的反萃取表观速率常数k2。结果表明:在优化的实验条件下(pH为7,手性载体与Cle浓度比为1∶4),Cle外消旋体能被含有手性载体D-( )-DBTA的厚体液膜有效拆分,分离因子大于1.08;Cle单体的跨膜迁移过程可以用两个串联的准一级不可逆过程进行描述。     

高效毛细管电泳对克伦特罗对映体的分离及定量检测

以羟丙基-β-环糊精(HP-β-CD)作为手性添加剂,采用毛细管区带电泳(CZE)成功分离了克伦特罗(Clenbuterol,Cle)对映体.研究了β-CD种类与浓度,缓冲液pH值及浓度,操作温度等对分离的影响.结果表明,以30 mmol/L的HP-β-CD为手性添加剂,50 mmol/L的磷酸盐缓冲溶液(pH 2.5)为缓中液,分离电压24 kV,操作温度20℃,可使Cle对映体实现基线分离,其分离度为6.78.对拆分机理也进行了探讨,测定了HP-β-CD与两对映体的结合常数及热力学参数;对CZE定量能力(线性,精度)进行了考察,R和S型的线性相关系数都大于0.998,两者的相对标准偏差(RSD)均低于2.2%.