痕量样品高灵敏度快速测量方法与便携式系统研究

为了实现高灵敏度、快速、准确鉴定痕量病原菌核酸样品,介绍了一种痕量核酸样品高灵敏度快速测量方法,并发展了一种微流控芯片核酸等温扩增实时分子诊断技术,研制了新型微纳体系生化反应载体芯片。通过表面惰性处理降低芯片表面对生物分子的吸附影响,构建一种大数值孔径、长工作距离的便携式共焦光学检测系统,有效消除背景荧光的影响,提高了检测灵敏度。在微纳升(7μL～40nL)试剂消耗反应体系水平,实现检测灵敏度5个DNA分子拷贝数,并以呼吸道感染疾病的大肠埃希氏菌检测为例,开展临床应用研究,满足低成本临床医疗应用需要。     

Label-free biomolecular imaging using scanning spectral interferometry

This letter presents a label-free biomolecular imaging technique based on white-light interferometry and spectral detection. The method measures thickness changes caused by specific binding between biomolecules to detect the presence of certain analyte. A spectrum-shifting algorithm is developed to resolve the thickness information from the spectrum. The axial resolution of the experimental instrument can reach ~1 nm, thereby enabling detection of trace amounts （-1 ng/mm2） of proteins or DNA. This letter also presents two experiments to prove the feasibility of the method for detecting proteins and DNA without fluorescent labeling.