污染水产去甲基化表观遗传毒性EGFP评价方法

pEGFP-C3质粒经过体外人工甲基化处理后,被转染进入HepG2细胞以构建重组细胞株．以5-AZA为阳性去甲基化毒物与重组细胞共培养,通过亚硫酸氢钠测序法定量检测EGFP基因启动子区甲基化状态,通过实时定量PCR检测EGFP基因表达,借助流式细胞术和荧光摄片定量检测共培养细胞的绿色荧光强度,在DNA甲基化、EGFP基因mRNA表达、GFP蛋白等多个层次研究5-AZA染毒处理与其去甲基化能力和荧光表达改变的响应关系．对天津污染水产的去甲基化能力进行了实际样品测试．结果表明,5-AZA与重组细胞的DNA甲基化、基因表达、蛋白产物变化之间存在显著关联,具有较低的检出浓度和良好的重复性．天津污染海域的水产去甲基化能力较强．本文初步建立了一种污染物去甲基化表观遗传毒性评价方法．     

Cooper Molecules: Second Pairing of Cooper Pairs in Gapless Superconductor CeCoIn_5

We establish a quantum field theory of phase transitions in gapless superconductor CeCoIn5.It is found that uniform Cooper pair gases with pure gradient interactions with negative coefficient can undergo a BardeenCooper-Schrieffer (BCS) condensation below a critical temperature.In the BCS condensation state,bare Cooper pairs with opposite wave vectors are bound into Cooper molecules,and uncoupled bare Cooper pairs are transformed into a new kind of quasiparticle,i.e.,the dressed particles.The Cooper molecule s.ystem is a condensate or a superfluid,and the dressed particle s.ystem is a normal fluid.The critical temperature is derived anal.yticall.y.The critical temperature of the superconductor CeCoIn5 is obtained to be T_c = 2.289 K,which approaches the experimental data.The transition from the BCS condensation state to the normal state is a first-order phase transition.