胰岛素样生长因子-1对血管平滑肌细胞PI3K／PTEN信号通路的影响

目的：探讨胰岛素样生长因子-1(IGF-1)促血管平滑肌细胞(VSMC)增殖的细胞内信号转导机制。方法：体外培养的兔血管平滑肌细胞分3组处理，以细胞计数、噻唑盐比色法测定细胞增殖能力，以磷脂酰肌醇-3激酶(PI3K)特异性抑制剂渥漫青霉素(WT)孵育细胞间接反映PI3K作用。Western Blot定量磷酸酶PTEN表达水平，免疫沉淀、特异底物diC16PIP3绿色试剂法测定PTEN脂质磷酸酶活性。结果：IGF-1(100μg／L)使细胞计数及MTT比色A值分别增加至对照组的2．8倍和3．8倍，WT抑制VSMC增殖，并完全逆转IGF-1的作用(均P<0.01)。各浓度IGF-1对PTEN蛋白表达水平无明显影响，其对PTEN活性的抑制呈浓度(10～100μg／L)及时间(3min～24h)依赖性(均P<0．01)。结论：IGF-I促VSMC增殖作用与活化PI3K蛋白激酶的促生长活性及抑制PTEN脂质磷酸酶的负性调节细胞生长作用有关。     

Fiber laser-pumped, chirped, PPMgLN-based high efficient broadband mid-IR generation

We demonstrate a high-efficiency, high-power-nanosecond fiber laser-pumped broadband mid-infrared out- put based on a linearly chirped PPMgLN crystal. By using a linearly polarized pulsed Yb-doped fiber laser as the pump, we experimentally obtain a 1.22-W broadband mid-infrared laser output under pump power of 10.15 W. The 3-dB bandwidths of the idler and signal output are approxiwately 150 and 13 nm, centering at 3.60 and 1.51 μm, respectively. The measured idler spectrum shows a smooth spectral profile.     

Isoscalar Giant Monopole Resonance in Relativistic Continuum Random Phase Approximation

The isoscalar giant monopole resonance （ISGMR） in nuclei is studied in the framework of a fully consistent relativistic continuum random phase approximation （RCRPA）. In this method the contribution of the continuum spectrum to nuclear excitations is treated exactly by the single particle Green＇s function technique. The negative energy states in the Dirac sea are also included in the single particle Green＇s function in the no-sea approximation. The single particle Green＇s function is calculated numerically by a proper product of the regular and irregular solutions of the Dirac equation. The strength distributions in the RCRPA calculations, the inverse energyweighted sum rule m-1 and the centroid energy of the ISGMR in ^120Sn and ^208Pb are analysed. Numerical results of the RCRPA are checked with the constrained relativistic mean field model and relativistic random phase approximation with a discretized spectrum in the continuum. Good agreement between them is achieved.     

一种基于增益调制技术的全光纤化脉冲Yb光纤激光器

以波长为975 nm的半导体激光器作为泵浦源,周期性地脉冲泵浦一个包含Yb掺杂光纤和光纤光栅对的Yb光纤激光器,实现了基于增益调制技术的全光纤化高功率Yb光纤激光器的稳定脉冲输出.在50 kHz重频下,采用20 W的泵浦功率和2.4 μs的泵浦脉冲宽度,获得了1 060 nm波长脉冲宽度仅100 ns的稳定脉冲激光输出,单脉冲激光能量约为20 μJ.以此作为脉冲激光种子进行功率放大,获得了性能稳定的全光纤结构高功率脉冲激光输出,放大后单脉冲能量超过200 μJ,激光放大器斜率效率达到60%.     

Collective multipole excitations of exotic nuclei in relativistic continuum random phase approximation

The isoscalar and isovector collective multipole excitations in exotic nuclei are studied in the framework of a fully self-consistent relativistic continuum random phase approximation （RCRPA）. In this method the contri- bution of the continuum spectrum to nuclear excitations is treated exactly by the single particle Green＇s function. Different from the cases in stable nuclei, there are strong low-energy excitations in neutron-rich nuclei and proton-rich nuclei. The neutron or proton excess pushes the centroid of the strength function to lower energies and increases the fragmentation of the strength distribution. The effect of treating the contribution of continuum exactly is also discussed.     

Relativistic Continuum Random Phase Approximation and Applications I. Formalism

A fully consistent relativistic continuum random phase approximation （RCRPA） is constructed in terms of the Green＇s function technique. In this method the contribution of the continuum spectrum to nuclear excitations is treated exactly by the single particle Green＇s function, which includes also the negative states in the Dirac sea in the no sea approximation. The theoretical formalism of RCRPA and numerical details are presented. The single particle Green＇s function is calculated numerically by a proper product of regular and irregular solutions of the Dirac equation. The numerical details and the formalism of RCRPA in the momentum representation are presented.     

Relativistic Continuum Random Phase Approximation and Applications II. Applications

The fully consistent relativistic continuum random phase approximation （RCRPA） has been constructed in the momentum representation in the first part of this paper. In this part we describe the numerical details for solving the Bethe-Salpeter equation. The numerical results are checked by the inverse energy weighted sum rules in the isoscalar giant monopole resonance, which are obtained from the constraint relativistic mean field theory and also calculated with the integration of the RCRPA strengths. Good agreement between them is achieved. We study the effects of the self-consistency violation, particularly the currents and Coulomb interaction to various collective multipole excitations. Using the fully consistent RCRPA method, we investigate the properties of isoscalar and isovector collective multipole excitations for some stable and exotic from light to heavy nuclei. The properties of the resonances, such as the centroid energies and strength distributions are compared with the experimental data as well as with results calculated in other models.