胰高血糖素样肽-1受体胞外区域突变体复合物的动力学研究

采用分子动力学模拟方法研究了胰高血糖素样肽-1(GLP-1)与GLP-1受体(GLP-1R)胞外区域的相互作用.结果表明,配体的结合导致受体的构象发生改变,Loop2区域的氨基酸Pro90和Trp91以及C末端的Glu128向配体移动.根据保守位点突变受体(P73A,V81L,Y88A,P90A和W91A)后所得多肽模拟数据,发现Loop2区域在突变体中的结构和柔性均发生了明显变化,Trp91和Tyr88的突变将导致配体亲和力丧失.研究结果证明,P73A突变型受体和野生型受体分别与配体相互作用后,二者数值差别不大,因此Pro73不是关键残基;V81L突变体则会导致配体亲和力的丧失.该结果为GLP-1药物设计提供了重要理论依据.     

Two-dimensional microscanner for laser projection

The design of a two-dimensional(2D) microscanner actuated electrostatically is presented,and a silicon-oninsulator(SOI) micromachining process is utilized to fabricate the sample.The microscanner can oscillate at inherent frequencies of 1146 and 360 Hz around two rotational axes,generating maximum twisting angles of ±10 and ±5.3 under two 10-V square waves,respectively.A monochromatic laser projection system based on Lissajous pattern is demonstrated using the developed microscanner,revealing an image resolution of 168×56 at 20 frames per second.