CYP2C9酶与Warfarin结合模型的立体选择性理论研究

对CYP2C9酶与S-Warfarin复合物的晶体结构进行分子对接、分子动力学模拟、通道分析及结合自由能计算,发现原晶体结构中的结合模式为"亚稳态",提出了CYP2C9与S-Warfarin结合的可催化模式;比较了CYP2C9与S-和R-Warfarin结合的异同,确定了在结合过程中起重要作用的锚定氨基酸残基,尤其是位于活性位点区域的苯丙氨酸簇.在结合过程中这些残基通过芳香环的移动对稳定底物的结合模式起到至关重要的作用,阐明了该酶呈现相关底物选择性的原因.对于CYP2C9与底物对接模式及立体选择性的研究有助于在分子层面上理解特异性底物与酶的结合特点,为潜在的药物设计提供了合理可信的理论依据.     

High-peak-power,single-mode,nanosecond pulsed,all-fiber laser for high resolution 3D imaging LIDAR system

This study presents an eye-safe, single-mode, nanosecond-pulsed, and all-fiber laser source with master- oscillator-power-amplifier configuration at 1 550 nm that is suitable for high-resolution three-dimensional (3D) imaging light detection and ranging (LIDAR) system. The output peak power of 7.6 kW is obtained at the 1.2-ns pulse width and 50-kHz repetition rate. The single-mode pulse laser output ensures the range precision and imaging results of the LIDAR system. The laser is used as a transmitter for the 3D imaging LIDAR system. The detailed characteristics of the LIDAR system and the results of the 3D imaging are presented.