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Elaborate calibration procedure for cell irradiation at the CAS-LIBB single-particle microbeam 下载免费PDF全文
Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro. It is crucial to find a reference point that relates the microbeam's location to the microscope's plane, and align
individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam,which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup. 相似文献
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This paper theoretically studies the free energy and conformational entropy of a long
polymer threading a long nanopore (n0/N \ge 0.1) on external electric
field. The polymer expanded model is built in this paper, that is, a
single long polymer chain with N monomers (each of size a) threading
a pore
with n0 monomers can be regarded as polymer with N+n_{0} monomers
translocating a 2-dimension hole embedded in membrane. A theoretical
approach is presented which explicitly takes into account the nucleation
theory. Our calculations imply that, the structure of polymer changes
more acutely than other situation, while its leading monomer reaches the second
vacuum and its end monomer escapes the first vacuum. And it is also shown
that the length scale of polymer and pore play a very important role for
polymer translocation dynamics. The present model predicts that the
translocation time depends on the chemical potential gradient and the
property of the solvent on sides of pore to some extent. 相似文献
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