Selenium and nutrition: The accuracy and variability of the selenium content in commercial supplements

Summary Selenium is a required trace-element that has been found to be protective against serious chronic diseases such as cancer and cardiovascular disease in some, but not all, epidemiological studies using both case-control and intervention designs. As a result, the fraction of the adult U.S. population now taking a daily selenium supplement is steadily increasing. In this study, we analyzed 10 or more replicate Se supplement tablets, from each of 15 different products representing 12 different brand names with most being sampled at two different times separated by approximately 30 months. Two chemical forms, seleno-yeast and selenate were tested in 50, 100 and 200 µg/tablet dosages (seleno-yeast) and 25 and 200 µg/tablet dosages (selenate). Variations in contemporary lots were evaluated at both sampling periods. The Se content provided on the product label is generally understated. One tablet contained 2.5 times more selenium than the stated dose. Selenate supplements are less accurately labeled and more highly variable compared to yeast supplements. One popular multivitamin, labeled at 200 µg/tablet, contained tablets in excess of 300 µg. Many subjects using this supplement will exceed the 400 µg/day tolerable upper limit of intake, recently established, for Se by the Institute of Medicine’s Food and Nutrition Board.     

Organization in lipid membranes containing cholesterol

A fundamental attribute of raft formation in cell membranes is lateral separation of lipids into coexisting liquid phases. Using fluorescence microscopy, we observe spontaneous lateral separation in free-floating giant unilamellar vesicles. We record coexisting liquid domains over a range of composition and temperature significantly wider than previously reported. Furthermore, we establish correlations between miscibility in bilayers and in monolayers. For example, the same lipid mixtures that produce liquid domains in bilayer membranes produce two upper miscibility critical points in the phase diagrams of monolayers.     

Fluid analysis of an input control problem

A two-station network with controllable inputs and sequencing control, proposed by Wein (Oper. Res. 38:1065–1078, 1990), is analyzed. A control is sought to minimize holding cost subject to a throughput constraint. In a Lagrangian formulation, input vanishes in the fluid limit. Several alternative fluid models, including workload formulations, are analyzed to develop a heuristic policy for the stochastic network. Both the fluid heuristic and Wein’s diffusion solution are compared with the optimal policy by solving the dynamic program. Examples with up to six customer classes, using Poisson arrival and service processes, are presented. The fluid heuristic does well at sequencing control but the diffusion gives additional, and better, information on input control. The fluid analysis, in particular whether the fluid priorities are greedy, aids in determining whether the fluid heuristic contains useful information.     

Cobalt-catalyzed aminocarbonylation of (hetero)aryl halides promoted by visible light

The catalytic aminocarbonylation of (hetero)aryl halides is widely applied in the synthesis of amides but relies heavily on the use of precious metal catalysis. Herein, we report an aminocarbonylation of (hetero)aryl halides using a simple cobalt catalyst under visible light irradiation. The reaction extends to the use of (hetero)aryl chlorides and is successful with a broad range of amine nucleophiles. Mechanistic investigations are consistent with a reaction proceeding via intermolecular charge transfer involving a donor–acceptor complex of the substrate and cobaltate catalyst.

An aminocarbonylation of (hetero)aryl halides using a simple cobalt catalyst under visible light irradiation is presented.     

Miscibility phase diagrams of giant vesicles containing sphingomyelin

Saturated sphingomyelin (SM) lipids are implicated in lipid rafts in cell plasma membranes. Here we use fluorescence microscopy to observe coexisting liquid domains in vesicles containing SM, an unsaturated phosphatidylcholine lipid (either DOPC or POPC), and cholesterol. We note similar phase behavior in a model membrane mixture without SM (DOPC/DPPC/Chol), but find no micron-scale liquid domains in membranes of POPC/PSM/Chol. We delineate the onset of solid phases below the miscibility transition temperature, and detail indirect evidence for a three-phase coexistence of one solid and two liquid phases.     

Fluorescent probes alter miscibility phase boundaries in ternary vesicles

We use 2H NMR to study the effects of probes on the miscibility transition in multilamellar vesicles of di(18:1) phosphatidylcholine (PC; DOPC), chain perdeuterated di(16:0)PC (DPPCd62), and cholesterol both with and without 0.5 mol % of the fluorescent probes DiIC12 and DiOC18. Both probes raise the miscibility transition temperature in dispersions of 1:1 DOPC/DPPCd62 + 30% cholesterol but to differing extents. In membranes containing the popular probe DiIC12, the fraction of DPPCd62 lipids in the liquid disordered phase is increased, and the ordering of that phase is reduced even at low temperatures. All findings are consistent with a probe-induced expansion of the entire miscibility phase boundary. We examine membranes with smaller DiIC12 fractions and find a significant increase in transition temperature for samples with 0.05 mol % DiIC12, demonstrating that trace components can dramatically alter membrane phase behavior.     

Far‐Red Organic Fluorophores Contain a Fluorescent Impurity

Far‐red organic fluorophores commonly used in traditional and super‐resolution localization microscopy are found to contain a fluorescent impurity with green excitation and near‐red emission. This near‐red fluorescent impurity can interfere with some multicolor stochastic optical reconstruction microscopy/photoactivated localization microscopy measurements in live cells and produce subtle artifacts in chemically fixed cells. We additionally describe alternatives to avoid artifacts in super‐resolution localization microscopy.     

Monotone control of queueing networks

This paper uses submodularity to obtain monotonicity results for a class of Markovian queueing network service rate control problems. Nonlinear costs of queueing and service are allowed. In contrast to Weber and Stidham [14], our monotonicity theorem considers arbitrary directions in the state space (not just control directions), arrival routing problems, and certain uncontrolled service rates. We also show that, without service costs, transition-monotone controls can be described by simple control regions and switching functions. The theory is applied to queueing networks that arise in a manufacturing system that produces to a forecast of customer demand, and also to assembly and disassembly networks.     

Zero-Inventory Conditions for a Two-Part-Type Make-to-Stock Production System

We consider the dynamic scheduling of a two-part-type make-to-stock production system using the model of Wein [12]. Exogenous demand for each part type is met from finished goods inventory; unmet demand is backordered. The control policy determines which part type, if any, to produce at each moment; complete flexibility is assumed. The objective is to minimize average holding and backorder costs. For exponentially distributed interarrival and production times, necessary and sufficient conditions are found for a zero-inventory policy to be optimal. This result indicates the economic and production conditions under which a simple make-to-order control is optimal. Weaker results are given for the case of general production times.