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Labeling of genetically modified organisms (GMOs) is now in place in many countries, including the European Union, in order to guarantee the consumer's choice between GM and non-GM products. Screening of samples is performed by polymerase chain reaction (PCR) amplification of regulatory sequences frequently introduced into genetically modified plants. Primers for the 35S promoter from Cauliflower mosaic virus (CaMV) are those most frequently used. In virus-infected plants or in samples contaminated with plant material carrying the virus, false-positive results can consequently occur. A system for real-time PCR using a TaqMan minor groove binder probe was designed that allows recognition of virus coat protein in the sample, thus allowing differentiation between transgenic and virus-infected samples. We measured the efficiency of PCR amplification, limits of detection and quantification, range of linearity, and repeatability of the assay in order to assess the applicability of the assay for routine analysis. The specificity of the detection system was tested on various virus isolates and plant species. All 8 CaMV isolates were successfully amplified using the designed system. No cross-reactivity was detected with DNA from 3 isolates of the closely related Carnation etched ring virus. Primers do not amplify plant DNA from available genetically modified maize and soybean lines or from different species of Brassicaceae or Solanaceae that are natural hosts for CaMV. We evaluated the assay for different food matrixes by spiking CaMV DNA into DNA from food samples and have successfully amplified CaMV from all samples. The assay was tested on rapeseed samples from routine GMO testing that were positive in the 35S screening assay, and the presence of the virus was confirmed. 相似文献
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Nevenka Rajic Djordje Stojakovic Natasa Zabukovec Logar Venceslav Kaucic 《Applied Surface Science》2010,257(5):1524-1532
The natural zeolite tuff (clinoptilolite) from a Serbian deposit has been studied as adsorbent for Ni(II) ions from aqueous solutions. Its sorption capacity at 298 K varies from 1.9 mg Ni g−1 (for the initial solution concentration of 100 mg Ni dm−3) to 3.8 mg Ni g−1 (for C0 = 600 mg Ni dm−3) and it increases 3 times at 338 K. The sorption is best described by the Sips isotherm model. The sorption kinetics follows the pseudo-second-order model, the activation energies being 7.44, 5.86, 6.62 and 6.63 kJ mol−1 for C0 = 100, 200, 300 and 400 mg Ni dm−3, respectively. The sorption involves a film diffusion, an intra-particle diffusion, and a chemical cation-exchange between the Na+ ions of clinoptilolite and the Ni2+ ions. The sorption is endothermic (ΔH° being 37.9, 33.4, 30.0, 27.7 and 24.3 kJ mol−1 for C0 = 100, 200, 300, 400 and 600 mg Ni dm−3, respectively) and spontaneous in the 298-338 K temperature range. Thermal treatment of the Ni(II)-loaded clinoptilolite results in the formation of spherical nano-NiO particles of approx. 5 nm in diameter which are randomly dispersed in the clinoptilolite lattice. 相似文献
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We consider the normalized Ricci flow ? t g = (ρ ? R)g with initial condition a complete metric g 0 on an open surface M where M is conformal to a punctured compact Riemann surface and g 0 has ends which are asymptotic to hyperbolic cusps. We prove that when χ(M) < 0 and ρ < 0, the flow g(t) converges exponentially to the unique complete metric of constant Gauss curvature ρ/2 in the conformal class. 相似文献
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Blagojević SM Anić SR Cupić ZD Pejić ND Kolar-Anić LZ 《Physical chemistry chemical physics : PCCP》2008,10(44):6658-6664
The influence of the initial malonic acid concentration [MA]0 (8.00 x 10(-3) < or = [MA]0 < or = 4.30 x 10(-2) mol dm(-3)) in the presence of bromate (6.20 x 10(-2) mol dm(-3)), bromide (1.50 x 10(-5) mol dm(-3)), sulfuric acid (1.00 mol dm(-3)) and cerium sulfate (2.50 x 10(-3) mol dm(-3)) on the dynamics and the kinetics of the Belousov-Zhabotinsky (BZ) reactions was examined under batch conditions at 30.0 degrees C. The kinetics of the BZ reaction was analyzed by the earlier proposed method convenient for the examinations of the oscillatory reactions. In the defined region of parameters where oscillograms with only large-amplitude relaxation oscillations appeared, the pseudo-first order of the overall malonic acid decomposition with a corresponding rate constant of 2.14 x 10(-2) min(-1) was established. The numerical results on the dynamics and kinetics of the BZ reaction, carried out by the known skeleton model including the Br2O species, were in good agreement with the experimental ones. The already found saddle node infinite period (SNIPER) bifurcation point in transition from a stable quasi-steady state to periodic orbits and vice versa is confirmed by both experimental and numerical investigations of the system under consideration. Namely, the large-amplitude relaxation oscillations with increasing periods between oscillations in approaching the bifurcation points at the beginning and the end of the oscillatory domain, together with excitability of the stable quasi-steady states in their vicinity are obtained. 相似文献
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Perišić-Janjić Nada U. Ž. Jovanović Bratislav Janjić Natasa J. Rajković Olga S. Antonović Dušan G. 《平面色谱法杂志一现代薄层色谱法》2003,16(6):425-432
JPC – Journal of Planar Chromatography – Modern TLC - The chromatographic behavior of nine newly synthesized s-tri-azines has been studied by thin-layer chromatography on C18/UV254... 相似文献
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Jevremovic M Lazarevic N Pavlovic S Orlic M 《Isotopes in environmental and health studies》2011,47(1):87-92
The objective of this study is to evaluate natural and artificial radioactivity levels in 18 different samples of medicinal herbs from Serbian market. The activity concentrations of (238)U, (232)Th, (40)K, and (137)Cs were measured using gamma spectroscopy system with a high-purity germanium detector. The activity concentrations of (238)U, (232)Th, (40)K, and (137)Cs were found to be in a range of 0.6-8.2, 1.7-15.1, 126-1243.7, and 0.3-8.8 Bq/kg, respectively. The annual whole body doses from ingestion of (137)Cs and natural radionuclides for an adult person who consumed herbal tea from these medicinal herbs were found to be 2.5-469.9 nSv for (137)Cs, 0.7-9.7 nSv for (238)U, 0.3-2.8 nSv for (232)Th, and 1026.0-10,132.0 nSv for (40)K. These doses are not hazardous to public health. The obtained results were compared with correspondent studies conducted worldwide. 相似文献
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Velázquez in 1994 used the degree theory to show that there is a perturbation of Simons’ cone, starting from which the mean curvature flow develops a type II singularity at the origin. He also showed that under a proper time-dependent rescaling of the solution around the origin, the rescaled flow converges in the C0 sense to a minimal hypersurface which is tangent to Simons’ cone at infinity. In this paper, we prove that the rescaled flow actually converges locally smoothly to the minimal hypersurface, which appears to be the singularity model of the type II singularity. In addition, we show that the mean curvature of the solution blows up near the origin at a rate which is smaller than that of the second fundamental form. 相似文献
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Panagiota Daskalopoulos Natasa Sesum 《Calculus of Variations and Partial Differential Equations》2010,37(1-2):187-215
We consider a compact star-shaped mean convex hypersurface ${\Sigma^2\subset \mathbb{R}^3}$ . We prove that in some cases the flow exists until it shrinks to a point. We also prove that in the case of a surface of revolution which is star-shaped and mean convex, a smooth solution always exists up to some finite time T < ∞ at which the flow shrinks to a point asymptotically spherically. 相似文献