Bestimmung von Zink in Thrombocyten mit INAA und AAS

Zusammenfassung Zur Klärung widersprüchlicher Angaben in der Literatur untersuchten wir die Zinkkonzentration von normalen menschlichen Thrombocyten mit Hilfe der instrumentellen Neutronenaktivierungsanalyse (INAA, n=26) und der Atomabsorptionsspektrophotometrie (AAS, n=5 × 6). Wir fanden folgende Fehlerquellen: Zinkkontamination, fehlerhaftes Thrombocytenfeuchtgewicht, Fehler bei der INAA. In den AAS-Proben (n=5 × 6) untersuchten wir zusätzlich die Konzentrationen von Calcium und Magnesium. Wir stellten fest, daß nach Ausschalten der Fehlerquellen die Normalbereiche (¯x ± 2 s) der Elemente Zink, Calcium und Magnesium viel enger liegen als bisher angenommen. Sie betragen, auf das plasmakorrigierte Thrombocytenfeuchtgewicht bezogen: Zn=26,5–32,3 g/g; Ca= 567–693 g/g; Mg=178–218 g/g.

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Determination of zinc in platelets by INAA and AAS

Summary To clarify discrepancies in the literature we determined the concentration of zinc in normal human platelets using instrumental neutron activation analysis (INAA, n=26) and atomic absorption spectrophotometry (AAS, n=5 × 6). We detected the following sources of error: zinc contamination, erroneous platelet wet weight, errors when using INAA. Additionally, we determined the concentrations of calcium and magnesium in the AAS-samples (n=5 × 6). We observed that after elimination of the sources of error the normal ranges (mean ± 2 SD) for the elements zinc, calcium and magnesium are far closer than hitherto believed. Based on platelet wet weight corrected for plasma, they are: Zn=26.5–32.3 g/g; Ca= 567-693 g/g, Mg =178–218 g/g.

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Herrn Prof. Dr. W. Fresenius zum 70. Geburtstag gewidmet     

Chemical constituents from the leaves of Manglietia phuthoensis and their effects on osteoblastic MC3T3-E1 cells

Two new phenyl glycosides, mangliesides A and B (1, 2), a new ionol glycoside, manglieside C (3), two new lignan glycosides, mangliesides D and E (4, 5), were isolated from the leaves of Manglietia phuthoensis, along with two known lignans, 3-methoxymagnolol (6) and obovatol (7). Their structures were established by means of 1D and 2D NMR, electrospray ionization (ESI)-MS and HR-ESI-MS experiments. Among them, compounds 2 and 5 significantly (p<0.05) increased the growth and differentiation of osteoblastic MC3T3-E1 cells in vitro.     

The stringy E-function of the moduli space of rank 2 bundles over a Riemann surface of genus 3

We compute the stringy E-function (or the motivic integral) of the moduli space of rank 2 bundles over a Riemann surface of genus 3. In doing so, we answer a question of Batyrev about the stringy E-functions of the GIT quotients of linear representations.

     

Lupane-triterpene carboxylic acids from the leaves of Acanthopanax trifoliatus

Two new lupane-triterpene carboxylic acids, called acantrifoic acid A (1) and acantrifoside C (2) have been isolated from the leaves of Acanthopanax trifoliatus. Based on extensive 1D and 2D NMR spectroscopic data, their chemical structures were determined as 3 alpha-acetoxy-30-hydroxylup-20(29)-ene-23,28-dioic acid and 3 alpha-acetoxy-30-hydroxylup-20(29)-ene-23,28-dioic acid 28-O-alpha-L-rhamnopyranosyl-(1-->4)-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranosyl ester.     

Muurolane‐type sesquiterpenes from marine sponge Dysidea cinerea

Seven new muurolane‐type sesquiterpenes, (4R,5R)‐muurol‐1(6),10(14)‐diene‐4,5‐diol (1), (4R,5R)‐muurol‐1(6)‐ene‐4,5‐diol (2), (4R,5R,10R)‐10‐methoxymuurol‐1(6)‐ene‐4,5‐diol (3), (4S)‐4‐hydroxy‐1,10‐seco‐muurol‐5‐ene‐1,10‐dione (4), (4R)‐4‐hydroxy‐1,10‐seco‐muurol‐5‐ene‐1,10‐dione (5), (6S,10S)‐6,10‐dihydroxy‐7,8‐seco‐2,8‐cyclo‐muurol‐4(5),7(11)‐diene‐12‐oic acid (6), and (6R,10S)‐6,10‐dihydroxy‐7,8‐seco‐2,8‐cyclo‐muurol‐4(5),7(11)‐diene‐12‐oic acid (7) were isolated from the marine sponge Dysidea cinerea. Their structures were determined by the combination of spectroscopic and chemical methods, including 1D‐NMR, 2D‐NMR, and CD spectra as well as by comparing the NMR data with those reported in the literature. Copyright © 2013 John Wiley & Sons, Ltd.     

Intersection pairings on singular moduli spaces of bundles over a Riemann surface and their partial desingularisations

This paper studies intersection theory on the compactified moduli space of holomorphic bundles of rank n and degree d over a fixed compact Riemann surface of genus where n and d may have common factors. Because of the presence of singularities we work with the intersection cohomology groups defined by Goresky and MacPherson and the ordinary cohomology groups of a certain partial resolution of singularities of Based on our earlier work [25], we give a precise formula for the intersection cohomology pairings and provide a method to calculate pairings on The case when n = 2 is discussed in detail. Finally Witten's integral is considered for this singular case.