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Mustafa A. Jihad Farah T. M. Noori Majid S. Jabir Salim Albukhaty Faizah A. AlMalki Amal A. Alyamani 《Molecules (Basel, Switzerland)》2021,26(11)
Flaky graphene oxide (GO) nanoparticles (NPs) were synthesized using Hummer’s method and then capped with polyethylene glycol (PEG) by an esterification reaction, then loaded with Nigella sativa (N. sativa) seed extract. Aiming to investigate their potential use as a smart drug delivery system against Staphylococcus aureus and Escherichia coli, the spectral and structural characteristics of GO-PEG NPs were comprehensively analyzed by XRD, AFM, TEM, FTIR, and UV- Vis. XRD patterns revealed that GO-PEG had different crystalline structures and defects, as well as a higher interlayer spacing. AFM results showed GONPs with the main grain size of 24.41 nm, while GONPs–PEG revealed graphene oxide aggregation with the main grain size of 287.04 nm after loading N. sativa seed extract, which was verified by TEM examination. A strong OH bond appeared in FTIR spectra. Furthermore, UV- Vis absorbance peaks at (275, 284, 324, and 327) nm seemed to be correlated with GONPs, GO–PEG, N. sativa seed extract, and GO –PEG- N. sativa extract. The drug delivery system was observed to destroy the bacteria by permeating the bacterial nucleic acid and cytoplasmic membrane, resulting in the loss of cell wall integrity, nucleic acid damage, and increased cell-wall permeability. 相似文献
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We studied effect of 1-aminoanthracene (1-AMA) binding on the structures of dimeric β lactoglobulin, dimeric odorant binding
protein (OBP) and monomeric α1-acid glycoprotein (lipocalin family proteins) by monitoring fluorescence excitation spectra and measuring fluorescence lifetimes
of the tryptophan residues of the proteins. Results show that binding of 1-AMA to β lactoglobulin and OBP modifies their conformation
even at low probe concentration compared to that of the proteins. Structural modification induces a red shift of the fluorescence
excitation spectra maximum of tryptophan residues accompanied with an increase of the third fluorescence lifetime and a decrease
of its pre-exponential factor. These effects were not observed for α1-acid glycoprotein, probably as the result of carbohydrate presence. These data raise doubts concerning use of 1-AMA as a
probe to study biological properties of β lactoglobulin and OBP. 相似文献
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Human serum albumin consists of a single polypeptide of 585 amino acid residues with 1 Trp residue. In the present work, we
measured fluorescence lifetimes of the protein in both native and denatured states. The results indicate that Trp emission
occurs with three lifetimes in both states. Lifetimes values and contribution to the global emission decay differ between
the two states. Data are interpreted as the results of an emission occurring from three substructures of the tryptophan formed
in the excited state. Two of these substructures are already present for the tryptophan free in solution. The third lifetime
is the result of the interaction between the tryptophan residue and surrounding microenvironment. The populations of these
substructures characterized by the pre-exponential parameters of the fluorescence lifetimes are dependent on the fluorophore
microenvironment and on the global protein structure. 相似文献
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Jihad René Albani 《Journal of fluorescence》2009,19(6):1061-1071
We measured fluorescence lifetimes of L-Tryptophan dissolved in de-ionized water and in ethanol in the absence and the presence
of high progesterone concentrations. The hormone absorbs between 220 and 280 with a peak around 250 nm, while its absorption
is equal to zero beyond 280 nm. Tryptophan excitation spectrum recorded in presence of progesterone shows that the So → 1La transition is completely abolished while the So
→
1Lb transition is not affected. Emission of L-tryptophan in water occurs with two fluorescence lifetimes, 0.40 and 2.8 ns. In
ethanol, three fluorescence lifetimes equal to around 0.2, 1.8 and 4.8 ns were observed. Addition of progesterone to the medium
does not affect any of the fluorescence lifetimes indicating clearly that both transitions could induce tryptophan excitation
and that recorded fluorescence lifetimes could be assigned to sub-structures generated in the excited state. 相似文献
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Grotjahn DB Sheridan PM Al Jihad I Ziurys LM 《Journal of the American Chemical Society》2001,123(23):5489-5494
Alkali metal amides typically aggregate in solution and the solid phase, and even in the gas phase. In addition, even in the few known monomeric structures, the coordination number of the alkali metal is raised by binding of Lewis-basic solvent molecules, with concomitant changes in structure. In contrast, the simplest lithium amide LiNH(2) has never been made in a monomeric form, even though its structure has been theoretically predicted several times. Here, the first experimental structural data for a monomeric, unsolvated lithium amide are determined using a combination of gas-phase synthesis and millimeter/submillimeter-wave spectroscopy. All data point to a planar structure for LiNH(2). The r(o) structure of LiNH(2) has a Li-N distance of 1.736(3) A, an N-H distance of 1.022(3) A, and a H-N-H angle of 106.9(1) degrees. These results are compared with theoretical predictions for LiNH(2), and experimental data for oligomeric, solid-phase samples, which could not resolve the question of whether LiNH(2) is planar or not. In addition, comparisons are made with revised gas-phase and solid-phase data and calculated structures of NaNH(2). 相似文献
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Jihad H. Asad 《Journal of Electrostatics》2013,71(4):754-755
This work showed that infinite d-dimensional networks consisting of identical capacitors each of capacitance C can be analyzed using basic concepts of physics. In this work we have showed that the equivalent capacitance Ceq between any two adjacent nodes in the infinite d-dimensional networks consisting of identical capacitors, is equal to dC where d is the dimension of the infinite network (i.e., d = 1,2,3,…). The results obtained here are in an excellent agreement with previous studied carried out. 相似文献
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Gregory B. Kharas Youya Gao Jihad Aburas Christina Chintanaphol Mallory L. Davis Hubert Dolubizno 《高分子科学杂志,A辑:纯化学与应用化学》2013,50(8):593-598
Novel trisubstituted ethylenes, ring-substituted butyl 2-cyano-3-phenyl-2-propenoates, RPhCH=C(CN)CO2C4H9 (where R is 2-C6H5CH2O, 3-C6H5CH2O, 4-C6H5CH2O, 4-CH3COO, 3-CH3CO, 4-CH3CO, 4-CH3CONH, 2-CN, 3-CN, 4-CN, 4-(CH3)2N, 4-(C2H5)2N) were prepared and copolymerized with styrene. The monomers were synthesized by the piperidine catalyzed Knoevenagel condensation of ring-substituted benzaldehydes and butyl cyanoacetate, and characterized by CHN analysis, IR, 1H and 13C-NMR. All the ethylenes were copolymerized with styrene (M1) in solution with radical initiation (ABCN) at 70°C. The compositions of the copolymers were calculated from nitrogen analysis and the structures were analyzed by IR, 1H and 13C-NMR. The order of relative reactivity (1/r1) for the monomers is 4-C6H5CH2O (6.39) > 2-C6H5CH2O (2.06) > 3-CH3CO (1.86) > 3-C6H5CH2O (1.78) > 4-CH3COO (1.58) > 3-CN (1.47) > 4-CN (1.21) > 4-(C2H5)2N (1.19) > 4-(CH3)2N (1.18) > 2-CN (1.04) > 4-CH3CO (0.71) > 4-CH3CONH (0.63). Decomposition of the copolymers in nitrogen occurred in two steps, first in the 200–500°C range with residue (3.6–9.5% wt), which then decomposed in the 500–800°C range. 相似文献