排序方式: 共有38条查询结果,搜索用时 15 毫秒
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Bereman MS Tomazela DM Heins HS Simonato M Cogo PE Hamvas A Patterson BW Cole FS MacCoss MJ 《Analytical and bioanalytical chemistry》2012,403(8):2397-2402
We report a method to measure in vivo turnover of four proteins from sequential tracheal aspirates obtained from human newborn infants with respiratory distress syndrome using targeted proteomics. We detected enrichment for all targeted proteins approximately 3 h from the start of infusion of [5,5,5-(2)H(3)] leucine, secretion times that varied from 1.2 to 2.5 h, and half lives that ranged between 10 and 21 h. Complement factor B, a component of the alternative pathway of complement activation, had an approximately twofold-longer half-life than the other three proteins. In addition, the kinetics of mature and carboxy-terminal tryptic peptides from the same protein (surfactant protein B) were not statistically different (p = 0.49). 相似文献
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Simonato S Gröger H Möllmer J Staudt R Puls A Dreisbach F Feldmann C 《Chemical communications (Cambridge, England)》2012,48(6):844-846
The CO(2) uptake on nanoscale AlO(OH) hollow spheres (260 mg g(-1)) as a new material is comparable to that on many metal-organic frameworks although their specific surface area is much lower (530 m(2) g(?1)versus 1500-6000 m(2) g(?1)). Suited temperature-pressure cycles allow for reversible storage and separation of CO(2) while the CO(2) uptake is 4.3-times higher as compared to N(2). 相似文献
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Carofiglio T Fornasier R Lucchini V Simonato L Tonellato U 《The Journal of organic chemistry》2000,65(26):9013-9021
Reductive amination of 6-deoxy-6-formyl-beta-cyclodextrin with 5-(p-aminophenyl)-10,15,20-tris(p-sulfonatophenyl)porphyrin in the presence of an excess of sodium cyanoborohydride affords the hydrophilic cyclodextrin-porphyrin conjugate 3 in 23% yield. The structure of 3 was confirmed by NMR spectroscopy and mass spectrometry techniques. Compound 3 showed a marked tendency to dimerize in aqueous conditions via the formation of intermolecular porphyrin-cyclodextrin inclusion complexes and/or through electrostatic interactions. Information on the structure of these aggregates has been obtained by the use of circular dichroism and UV-vis spectroscopy. Aggregation can be avoided by the use of heptakis(2,3,6-tri-O-methyl)-beta-cyclodextrin (TM beta CD) that forms a 1:1 inclusion complex with compound 3. 相似文献
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Laurence Latu-Romain Celine Mouchet Cyril Cayron Emmanuelle Rouviere Jean-Pierre Simonato 《Journal of nanoparticle research》2008,10(8):1287-1291
In this paper the effect of varying temperature, pressure and chemical precursors on the vapour–liquid–solid (VLS) growth
of silicon nanowires (Si NWs) have been investigated. Some aspects of nucleation and growth mechanisms are discussed. Control
on Si NW morphology by varying the choice of gaseous precursor (silane or dichlorosilane) at elevated temperatures is reported. 相似文献
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Liu FX Marchal-Roch C Bouchard P Marrot J Simonato JP Hervé G Sécheresse F 《Inorganic chemistry》2004,43(7):2240-2242
Silver atoms and strongly interlinking Keggin units ensure the tridimensionnal macrocationic structure of [Ag(6)(PMo(10)V(2)O(40))](CH(3)COO).8H(2)O, which catalyzes O(2)-based oxidation of p-methoxytoluene in acetic acid by air. 相似文献
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Manuela Simonato Aldo Baritussio Barbara Pioselli Carlo Ori Silvia Catinella Virgilio P. Carnielli Paola E. Cogo 《Analytical and bioanalytical chemistry》2014,406(25):6225-6233
Surfactant protein C (SP-C) is deemed as the surfactant protein most specifically expressed in type II alveolar epithelial cells and plays an important role in surfactant function. SP-C turnover in humans and its meaning in the clinical context have never been approached. In this study, we used mass spectrometry to investigate SP-C turnover in humans. We studied four infants and eight adults requiring mechanical ventilation. All patients had no lung disease. Patients received a 24-h continuous infusion of 13C-leucine as precursor of SP-C, and serial tracheal aspirates and plasma samples were obtained every 6 h till 48 h. SP-C was isolated from tracheal aspirates by sorbent-phase chromatography. 13C-leucine SP-C enrichment could be successfully measured in three infant and in four adult samples by using mass spectrometry coupled with a gas chromatographer. Median SP-C fractional synthesis rate, secretion time, and peak time were 15.7 (14.1–27.5) %/day, 6.0 (4.7–11.5) h, and 24 (20–27) h. In conclusion, this study shows that it is feasible to accurately determine SP-C turnover in humans by stable isotopes. 相似文献