An In Vitro Pilot Fermentation Study on the Impact of Chlorella pyrenoidosa on Gut Microbiome Composition and Metabolites in Healthy and Coeliac Subjects

The response of a coeliac and a healthy gut microbiota to the green algae Chlorella pyrenoidosa was evaluated using an in vitro continuous, pH controlled, gut model system, which simulated the human colon. The effect of C. pyrenoidosa on the microbial structure was determined by 16S rRNA gene sequencing and inferred metagenomics, whereas the metabolic activitywas determined by¹H-nuclear magnetic resonancespectroscopic analysis. The addition of C. pyrenoidosa significantly increased the abundance of the genera Prevotella, Ruminococcus and Faecalibacterium in the healthy donor, while an increase in Faecalibacterium, Bifidobacterium and Megasphaera and a decrease in Enterobacteriaceae were observed in the coeliac donor. C. pyrenoidosa also altered several microbial pathways including those involved in short-chain fatty acid (SCFA) production. At the metabolic level, a significant increase from baseline was seen in butyrate and propionate (p < 0.0001) in the healthy donor, especially in vessels 2 and 3. While acetate was significantly higher in the healthy donor at baseline in vessel 3 (p < 0.001) compared to the coeliac donor, this was markedly decreased after in vitro fermentation with C. pyrenoidosa. This is the first in vitro fermentation study of C. pyrenoidosa and human gut microbiota, however, further in vivo studies are needed to prove its efficacy.     

Evaluating alternative system configurations using simulation: A nonparametric approach

The real utility of simulation lies in comparing different alternatives that might represent competing system designs. Conventional statistical techniques are not directly applicable to the analysis of simulation output data in the evaluation of competing alternatives since the usual assumptions of normality and common variance are difficult to justify in simulation experiments. This paper revisits a known nonparametric test whose application has recently become feasible due to considerable increases in computing power:randomization tests assess the significance of the observed value of the test statistic by evaluating different permutations of the data. The procedure only requires invariance of the data under all permutations.     

The characterization of soils, clays, and clay firing products

Mössbauer spectroscopic studies of soils are generally centered around the characterization of Fe³⁺ oxides, making use of distinctive magnetic properties of the different oxides and the high sensitivity of the technique for magnetically ordered phases. Most work on clays and clay minerals, in contrast, focuses on the determination of the oxidation state of iron, iron structural site occupancies and distortions, and the transformations that take place during clay firing.     

The characterization of poorly chrystalline Si-containing natural iron oxides by Mössbauer spectroscopy

Soils developed from recent basalts of Marion Island in the Sub-Antarctic contain about 20% of a poorly crystalline iron oxide. The association of this phase with Al and Si appears to have a major influence on its Mössbauer spectra: whereas room-temperature spectra indicate a relatively regular structure, the magnetic hyperfine fields at 4.2K are lower than those of even the most poorly crystalline pure ferrihydrites.     

Niobian iron oxides as heterogeneous Fenton catalysts for environmental remediation

Heterogeneous Fenton or Fenton-like reagents consist of a mixture of an iron-containing solid matrix and a liquid medium with H₂O₂. The Fenton system is based on the reaction between Fe^2?+? and H₂O₂ to produce highly reactive intermediate hydroxyl radicals (^???OH), which are able to oxidize organic contaminants, whereas the Fenton-like reaction is based on the reaction between Fe^3?+? and H₂O₂. These heterogeneous systems offer several advantages over their homogeneous counterparts, such as no sludge formation, operation at near-neutral pH and the possibility of recycling the iron promoter. Some doping transition cations in the iron oxide structure are believed to enhance the catalytic efficiency for the oxidation of organic substrates in water. In this work, goethites synthesized in presence of niobium served as precursors for the preparation of magnetites (niobian magnetites) via chemical reduction with hydrogen at 400°C. These materials were used as Fenton-like catalysts. Both groups of (Nb, Fe)-oxide samples were characterized by ⁵⁷Fe Mössbauer spectroscopy at 298 K. The results show that increasing niobium contents raise the catalytic potential for decomposition of methylene blue, which was, in this work, used as a model molecule for organic substrates in water.     

The effect of the 4MAT method (learning styles and brain hemispheres) of instruction on achievement in mathematics

The purpose of this study was to determine the efficiency of 4MAT method of instruction in which learning style and cerebral hemispheres are taken into account in teaching the binary operation and its properties in mathematics. The sample of this study comprised 58 ninth grade students in two separate classes in a high school. One of the classes was selected as the experimental group in which 4MAT method of instruction was used; and the other class was selected as the control group in which the traditional teaching was given, and this selection was performed randomly. The data have been obtained primarily from three scales, namely ‘mathematical knowledge test’, ‘mathematical attitude scale’ and ‘knowledge test on binary operation and its properties’. It has been determined that 4MAT method of instruction was more efficient than the traditional method in teaching of the binary operation subject in mathematics.     

Multi-location transshipment problem with capacitated production

We consider coordination among stocking locations through replenishment strategies that explicitly take into account lateral transshipments, i.e., transfer of a product among locations at the same echelon level. Our basic contribution is the incorporation of supply capacity into the traditional transshipment model. Our goal is to analyze the impact on system behavior and on stocking locations’ performance of the fact that the supplier may fail to fulfill all the replenishment orders. We therefore formulate the capacitated supply scenario as a network flow problem embedded in a stochastic optimization problem, which is solved through a sample average approximation method. We find that, depending on the production capacity, system behavior can vary drastically. Moreover, in a production-inventory system, we find evidence that either capacity flexibility (i.e., extra production) or transshipment flexibility (i.e., pooling) is required to maintain a desired level of service.