Measuring intracellular calcium fluxes in high throughput mode

The measurement of intracellular calcium fluxes in real time is widely applied within the pharmaceutical industry to measure the activation of G-protein coupled receptors (GPCRhyp;s), either for pharmacological characterisation or to screen for new surrogate ligands. Initially restricted to G(q) coupled GPCRs, the introduction of promiscuous and chimeric G-proteins has further widened the application of these assays. The development of new calcium sensitive dyes and assays has provided sensitive, homogeneous assays which can be readily applied to high throughput screening (HTS). In this paper we describe the full automation of this assay type using a fluorometric imaging plate reader (FLIPR ) integrated into a Beckman/Sagian system to establish a simple robotic system that is well suited for the current medium throughput screening in this area of lead discovery. Using a recently completed HTS we discuss important determinants for FLIPR based screening, highlight some limitations of the current approach, and look at the requirements for future automated systems capable of keeping up with expanding compound files.     

Demonstrating Linear Regression and Error Using an Experiment with a Microwave Oven

To provide an application for the method of linear least squares to data collected in a laboratory, a beaker with water is heated in a microwave oven, and the water temperature is measured as a function of heating variables (time and oven setting). This procedure enables a student to obtain a regression line for each oven setting, and to evaluate the intercept and slope of this line and compare them with the initial temperature of the water and the heating versus oven setting relationship described in the microwaves manufacturers manual. They also are asked to identify any sources of errors observed in this experiment.     

Origin and System Effects of Polarization Mode Dispersion in Chirped Bragg Gratings

Polarization mode dispersion of chirped Bragg gratings is analyzed in terms of key birefringence phenomena and impact on telecommunication systems performance. The influence on polarization mode dispersion (PMD) of fiber birefringence, grating chromatic dispersion, and ripples of the group delay curve is pointed out. Polarization mode dispersion influence on systems performance is investigated by numerical simulations and transmission experiments at 10 Gbit/s. The deterministic nature of Bragg gratings PMD determines a moderate, upper-limited system penalty for a transmission line employing a single compensating device. However, in the case of broadband components, a non-negligible PMD penalty may be observed due to the difficulty of controlling accurately the group delay linearity.     

Origin and System Effects of Polarization Mode Dispersion in Chirped Bragg Gratings

Polarization mode dispersion of chirped Bragg gratings is analyzed in terms of key birefringence phenomena and impact on telecommunication systems performance. The influence on polarization mode dispersion (PMD) of fiber birefringence, grating chromatic dispersion, and ripples of the group delay curve is pointed out. Polarization mode dispersion influence on systems performance is investigated by numerical simulations and transmission experiments at 10 Gbit/s. The deterministic nature of Bragg gratings PMD determines a moderate, upper-limited system penalty for a transmission line employing a single compensating device. However, in the case of broadband components, a non-negligible PMD penalty may be observed due to the difficulty of controlling accurately the group delay linearity.     

Substructured two-grid and multi-grid domain decomposition methods

Two-level Schwarz domain decomposition methods are very powerful techniques for the efficient numerical solution of partial differential equations (PDEs). A two-level domain decomposition method requires two main components: a one-level preconditioner (or its corresponding smoothing iterative method), which is based on domain decomposition techniques, and a coarse correction step, which relies on a coarse space. The coarse space must properly represent the error components that the chosen one-level method is not capable to deal with. In the literature, most of the works introduced efficient coarse spaces obtained as the span of functions defined on the entire space domain of the considered PDE. Therefore, the corresponding two-level preconditioners and iterative methods are defined in volume. In this paper, we use the excellent smoothing properties of Schwarz domain decomposition methods to define, for general elliptic problems, a new class of substructured two-level methods, for which both Schwarz smoothers and coarse correction steps are defined on the interfaces (except for the application of the smoother that requires volumetric subdomain solves). This approach has several advantages. On the one hand, the required computational effort is cheaper than the one required by classical volumetric two-level methods. On the other hand, our approach does not require, like classical multi-grid methods, the explicit construction of coarse spaces, and it permits a multilevel extension, which is desirable when the high dimension of the problem or the scarce quality of the coarse space prevents the efficient numerical solution. Numerical experiments demonstrate the effectiveness of the proposed new numerical framework.

     

WDM-DPSK Systems Based on SOAs in TOSCA Project

Abstract

Using differential phase shift keying, we fully exploit semiconductor optical amplifiers (SOAs) for signal amplification in practical Wavelength Division Multiplexing (WDM) Nx10 Gbit/s unrepeated systems. Record transmission experiments are presented, thus making SOAs a competitive amplification alternative in metro and extended-metro networks.     

Quantum Optimal Control Problems with a Sparsity Cost Functional

In this article, the investigation of a class of quantum optimal control problems with L¹ sparsity cost functionals is presented. The focus is on quantum systems modeled by Schrödinger-type equations with a bilinear control structure as it appears in many applications in nuclear magnetic resonance spectroscopy, quantum imaging, quantum computing, and in chemical and photochemical processes. In these problems, the choice of L¹ control spaces promotes sparse optimal control functions that are conveniently produced by laboratory pulse shapers. The characterization of L¹ quantum optimal controls and an efficient numerical semi-smooth Newton solution procedure are discussed.     

All-optical 40 Gbits/s packet regeneration by means of cross-gain compression in a semiconductor optical amplifier

We experimentally demonstrate all-optical reshaping of 40 Gbits/s packets by using cross-gain compression in a semiconductor optical amplifier (SOA). This scheme, which is based on cross-saturation effects between two conjugate signals copropagating in a single SOA, is wavelength preserving and polarization independent and does not suffer from any transient effect at packet edges. We report evidence of noise redistribution and packet reshaping by means of the bit-error rate versus threshold measurements for different input optical signal-to-noise ratios.