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1.
We report the synthesis, characterization, thermal and dilute-solution properties of zwitterionic copolymers of the sulfobetaine type, where the proportion of ionic groups is varied over a wide range. Two different structures are considered: poly (2-vinyl pyridine sulfopropyl betaine) and poly (4-vinyl pyridine sulfopropyl betaine). Synthesis is carried out to obtain random copolymers which are further characterized and their ionic content determined. At low proportions of ionic groups, the behavior is similar to that observed in ionomers, with substantial differences between the two structures, Increased interaction among the zwitterionic groups at higher charge densities is evidenced, which leads to the formation of clusters and agglomerates. An increase in the intrinsic viscosity with salt content in aqueous solutions is also observed. This behavior is analogous to that of homopolymers with 100% ionic content, but with particular features of interest. 相似文献
2.
M. Costa-Ferreira A. Dias C. Maximo M. J. Morgado G. Sena-Martins J. Cardoso Duarte 《Applied biochemistry and biotechnology》1994,44(3):231-242
Production of xylanolytic enzymes by anAspergillus niger CCMI 850 isolate was investigated in batch cultures. The effect of the composition of a fermentation medium that did not
include chemical inducers, on β-xylanase, β-xylosidase, α-l-arabinofuranosidase, and total cellulase activity was studied. With 4% xylan as the carbon source, about 65 U/mL of β-xylanase
was obtained, whereas the total cellulase activity was undetectable, under the specified conditions. This β-xylanase activity
represents the highest reported for a wild-type strain ofA. niger. The effect of pH and temperature on the activity of β-xylanase was studied. Partial characterization of the β-xylanase showed
that with insoluble birchwood as substrate theK
m
andV
max were 0.3 mM and 19 μmol/min, respectively. Aspects of using the crude β-xylanase preparation for applications in the pulp and paper industry
were discussed. 相似文献
3.
Rodrigo Cardoso Magno Lorian Cobra Straker Wanderley de Souza Marcia Attias 《Microscopy and microanalysis》2005,11(2):166-174
Toxoplasma gondii, the causative agent of toxoplasmosis, is capable of actively penetrating and multiplying in any nucleated cell of warm-blooded animals. Its survival strategies include escape from fusion of the parasitophorous vacuole with host cell lysosomes and rearrangement of host cell organelles in relation to the parasitophorous vacuole. In this article we report the rearrangement of host cell organelles and elements of the cytoskeleton of LLCMK2 cells, a lineage derived from green monkey kidney epithelial cells, in response to infection by T. gondii tachyzoites. Transmission electron microscopy made on flat embedded monolayers cut horizontally to the apical side of the cells or field emission scanning electron microscopy of monolayers scraped with scotch tape before sputtering showed that association of mitochondria to the vacuole is much less frequent than previously described. On the other hand, all parasitophorous vacuoles were surrounded by elements of the endoplasmic reticulum. These data were complemented by observations by laser scanning microscopy using fluorescent probes from mitochondria and endoplasmic reticulum and reinforced by three-dimensional reconstruction from serial sections observed by transmission electron microscopy and labeling of mitochondria and endoplasmic reticulum by fluorescent probes. 相似文献
4.
L. A. Schuch D. J. R. Nordemann W. O. Barreto A. Cardoso A. Zago 《Journal of Radioanalytical and Nuclear Chemistry》1994,177(1):39-49
Soil samples from Paraná State, Brazil, were collected in 1991 and compared with others collected at the same location in March 1977 and at the end of 1983. Pedological analyses were practiced on these samples and137Cs and232Th,226Ra and40K activities were determined by gammaray spectrometry. A latitude dependence of137Cs was found as well. It was impossible to determine the137Cs contribution from Chernobyl nuclear accident because of low fallout and intense leaching, erosion and re-suspension in soils of regions with high annual precipitation. Natural radionuclides did not show such effects. 相似文献
5.
Teixeira-Neto É Leite CA Cardoso AH Medeiros da Silva MC Braga M Galembeck F 《Journal of colloid and interface science》2000,231(1):182-189
Poly(styrene-co-acrylamide) (PS-AAM) latex was prepared, fractionated by sedimentation under gravity, and characterized by PCS, infrared spectra, secondary and backscattered electron imaging in the scanning electron microscope, and electron spectroscopy imaging in an analytical transmission electron microscope. Three latex fractions were obtained. The lower fraction was opalescent and its particles were the more uniform, concerning size, chemical composition, and topochemical features. This lower fraction was still further fractionated by zonal centrifugation in a density gradient, yielding two fractions with similar macrocrystal-forming abilities but different sizes and chemical compositions. These results confirm those previously obtained for the PS-HEMA latex. Copyright 2000 Academic Press. 相似文献
6.
Ultraviolet (UV) spectrophotometric and nonaqueous volumetric methods are described for the determination of carvedilol in pharmaceutical formulations. Linearity, precision, and accuracy were evaluated according to the validation guidelines of the International Conference on Harmonization and the United States Pharmacopeia for both methods. The UV spectrophotometric procedure was performed in ethanol at 244 nm. Good linearity was obtained between 2 and 7 microg/mL with a correlation coefficient of 0.9999. The intra- and interday precision values were <2% for all samples analyzed. The accuracy, determined from recovery studies, was between 97.5 and 102.2%. The other procedure was based on the volumetric quantitation of carvedilol in a nonaqueous medium with 0.01 M perchloric acid and 1% violet crystal as the indicator. The validation of the volumetric method yielded good results that included linearity (r of > 0.999), precision (relative standard deviations of <2% for intra- and interday precision), and accuracy (96.4-102.4%). The methods were applied to tablets and compounded capsules. Statistical analysis by analysis of variance showed no significant difference between the results obtained by the proposed methods. 相似文献
7.
A252Cf neutron source has been used to analyse manganese in ores such as pyrolusite, rodonite (manganese silicate) and blends used in dry-batteries. Samples with about 150 mg and standards of manganese dioxide were irradiated for about 20 min and counted using a well-type NaI(Tl) scintillation counter and scaler, with or without pulse-height discriminator between the detector and the scaler. The interferences of nuclear reactions56Fe(n,p)56Mn and59Co(n,α)56Mn were studied, as well as problems in connection with neutron shadowing during irradiation, gamma-rays attenuation during counting and influence of granulometry of samples. Some of the samples were also analysed by wet-chemical method (sodium bismuthate) in order to compare results. 相似文献
8.
A flow-injection—conductimetric method was applied to the determination of ammonia, nitrate and nitrite at concentrations down to 5, 20 and 20 ng ml?1, respectively. Ammonia was determined by merging the injected sample with an alkaline solution (NaOHEDTA) and passing the mixture through a diffusion cell. The ammonia released was collected by a flowing stream of deionized water that passed through a conductance flow cell. Nitrate and nitrite concentrations were determined after reduction to ammonia in alkaline medium using a column filled with metallic zinc. The ammonia produced was then measured as described above. About 60 samples per hour can be processed with a relative standard deviation of about 1%. Satisfactory agreement was observed between results for ammonia in samples of natural water and nitrate in tap and mineral water determined by the proposed method and by standard spectrophotometric procedures. Speciation can be achieved by adding sulphanilic acid to remove nitrite from the sample and determining the ammonia without the use of the column. 相似文献
9.
Thamara R. dos Santos Jakcline dos Santos Melo Alysson V. dos Santos Patrícia Severino lvaro S. Lima Eliana B. Souto Aleksandra Zieliska Juliana C. Cardoso 《Molecules (Basel, Switzerland)》2022,27(24)
The aim of this study was the development of a cereal bar based on bee pollen (BP), honey (H), and flour by-products (peel passion fruit flour—PPFF), generating an innovative product. BP is a protein-rich ingredient and can be used in the composition of cereal bars. PPFF is a by-product rich in fibers. The formulations were developed using a 23 factorial design with four replicates in the center point, studying the sensory analysis as a response variable. The texture and nutritional parameters were performed for the optimal formulation. BP showed ca. 15% of protein. The final formulation (10.35% BP, 6.8% PPFF, and 25% H) presented 22.2% moisture, 1.8% ash, 0.4% total fat, 3.0% fiber, 63.1% carbohydrates, and 74.0 Kcal/25 g. The sensory analysis presented valued around 7 (typical of a traditional bar). Regarding the possibility of purchasing the product, 51% of the panelists said they would probably buy the developed product. The formulated cereal bar had a similar composition as those already marketed. Moreover, it can be considered a source of fiber and is sensory acceptable. This approach opens up new opportunities for developing nutritional and functional foodstuff with improved sensorial aspects. 相似文献
10.
Partial least squares model and design of experiments toward the analysis of the metabolome of Jatropha gossypifolia leaves: Extraction and chromatographic fingerprint optimization 下载免费PDF全文
Fausto Carnevale Neto Rafael Teixeira Freire Patrícia Cardoso Renato Lajarim Carneiro Vanderlan Da Silva Bolzani Ian Castro‐Gamboa 《Journal of separation science》2016,39(6):1023-1030
A major challenge in metabolomic studies is how to extract and analyze an entire metabolome. So far, no single method was able to clearly complete this task in an efficient and reproducible way. In this work we proposed a sequential strategy for the extraction and chromatographic separation of metabolites from leaves Jatropha gossypifolia using a design of experiments and partial least square model. The effect of 14 different solvents on extraction process was evaluated and an optimized separation condition on liquid chromatography was estimated considering mobile phase composition and analysis time. The initial conditions of extraction using methanol and separation in 30 min between 5 and 100% water/methanol (1:1 v/v) with 0.1% of acetic acid, 20 μL sample volume, 3.0 mL min?1 flow rate and 25°C column temperature led to 107 chromatographic peaks. After the optimization strategy using i‐propanol/chloroform (1:1 v/v) for extraction, linear gradient elution of 60 min between 5 and 100% water/(acetonitrile/methanol 68:32 v/v with 0.1% of acetic acid), 30 μL sample volume, 2.0 mL min?1 flow rate, and 30°C column temperature, we detected 140 chromatographic peaks, 30.84% more peaks compared to initial method. This is a reliable strategy using a limited number of experiments for metabolomics protocols. 相似文献