HEV嵌合抗原的克隆表达及在抗-HEV ELISA试剂中的应用

通过反转录从戊肝病人粪便中克隆了戊型肝炎病毒（HEV）第二（ORF2）和第三（ORF3）读码框区的抗原决定簇基因，利用基因工程手段，在大肠杆菌中表达了含有上述二种抗原的融合蛋白，该融合蛋白具有良好的抗原性和特异性，以该融合蛋白为抗原制备的抗－HEV ELISA试剂检测中国药品生物制品检定所40份标准血清时，其阴性、阳性符合率分别达29／30、10／10，符合国家标准。     

细胞动力学研究IV.细菌非理想生长过程的热动力学

By using LKB2277 Bioactivity Monitor, we have determined the thermogenesis power curves of four kinds of bacteria: Brucella M5(55010), 83-980, 83-981 and E. coli. We have also derived the thermokinetic equation for the process of bacterial growth, which was untypical "S" , unideal growth, as:
Ln[P•(1-P/Pm)?-1]=ln[P0•(1-P0/Pm)?-1]+kt
From this equation, the rate constants of bacterial growth k wore obtained. This thermokinetic equation is very suitable for cell growth of separated culture, in which the thermogenesis curve is untypical. The model propoted by equationl(1) has been compared with the exponential and logistic models. This thermokinetic equation is very significant for the study of bacterial limited growth and their characteristics.     

细胞动力学研究 III.细菌算术级数式生长过程热动力学

By using an LKB-2277 Bioactivity Monitor, we have determined the thermogenesis curves of two species of Brucellas arithmetic series growth. From the thermogenesis curves, a thermokinetic equation as:
dP/dt=km﹒Pn
could be established for bacterial arithmetic series growth in which the order of growth metabolism n=0. The mean specific rate constant of multiplication μand the mean generation time G et al. were calculated. This model was compared with the exponential model.     

细胞动力学研究 II.产物抑制生长过程的热动力学

By using an LKB2277 Bioactivity Monitor, we have determined the thermogenesis power curves of four kinds of Brucellas S2(55007), M5 (55009),83-202,83-980. And established the thermokinetic equation for the process of cell growth inhibited by the products of metabolism, as:
Ln[1/(β﹒Pt)-1]=ln[1/(β﹒P0)-1]-k0﹒t
From this equation, the rate constants of cell growth k [= k0(1 - β﹒P0)] were obtained. This thermokinetic equation is very suitable for cell growth of separated culture and is very important for the study of bacterial limited growth and their characteristics.