Interferometric testing at very high temperatures by TV holography (ESPI)

This paper describes how TV-holography or electronic-speckle-pattern interferometry (ESP) can be used to monitor and measure with interferometric sensitivity the surface behavior, vibrations and deformations of objects heated to temperatures up to +3000°C. If problems due to rapid microstructure changes and the object actually melting are disregarded, the temperature limits are determined by the accessible laser illumination due to the increasing background radiantion.     

Resolution of tryptophan-ANS fluorescence energy transfer in apomyoglobin by site-directed mutagenesis

Resonance energy transfer between tryptophanyl residues and the apolar fluorescent dye 1-anilino-8-naphthalene sulfonate (ANS) occurs when the fluorophore is bound to native folded sperm whale apomyoglobin. The individual transfer contribution of the two tryptophanyl residues (W7 and W14, both located on the A-helix of the protein) was resolved by measuring the tryptophan-ANS transfer efficiency for the ANS-apomyoglobin complexes formed by wild-type protein and protein mutants containing one or no tryptophanyl residues, i.e. W7F, W14F and W7YW14F. The transfer efficiency of W14 residue was found to be higher than that of W7, thus indicating that W14 acts as the main energy donor in the ANS-apomyoglobin complex. This suggests that the plane containing the anilinonaphthalene ring of the extrinsic fluorophore has a spatial orientation similar to that of W14 and, hence, to the heme group in the holoprotein.     

Torsional dynamics of the Birnboim-Schrag multiple lump resonator studied using TV-holography

We have studied the torsional dynamics of the Birnboim-Schrag multiple lump resonator (MLR) using TV holography. The relative torsional displacement amplitudes have been determined for all resonator lumps at their five resonance modes. Due to the low vibration amplitudes for the highest frequency mode, we only give a rough estimate of the relative torsional displacements for this resonance. For some lumps, the observed relative displacement amplitudes deviate by as much as a factor of 2 from the predictions of the dumbbell theory commonly used to analyze MLR dynamics. The experimental data agree well with the recent theoretical analysis carried out by Mikkelsen et al. (1992).