Spectral imaging of time-resolved anisotropy: theory and experiment

Time-resolved fluorescence anisotropy on the nanosecond time scale is useful for the study of the rapid rotation of macromolecules. A system combining the capabilities of fluorescence spectral imaging with time-resolved fluorescence anisotropy and enabling the wide-field measurement of the spectroscopic parameters of fluorophores is discussed. The phasor approach is used to quantitatively analyze the time-resolved fluorescence anisotropy by transforming the polarized parallel and perpendicular components to the phasor space in the frequency domain, respectively, and a unique way to calculate the fluorescence rotational correlation time is put forward. Experimental results prove that the phasor approach is a proper model for the time-resolved fluorescence anisotropy.     

基于全场干涉测量方法的透镜三维轮廓测量

为了满足高精度透镜加工工艺的要求,设计了一套基于全场干涉测量方法的透镜三维轮廓测量系统。已标定的透镜三维轮廓测量系统利用分布式反馈激光二极管对透镜进行波数扫描,对CCD相机采集到的干涉图像依次进行随机采样傅里叶变换和解卷绕算法,最终通过计算得出透镜的三维轮廓数据。实验结果表明:该系统横向和纵向的分辨率均为0.011 3 mm/pixels,深度方向的测量均方根误差为±19.8 nm。系统结构简单、稳定性高,对透镜没有任何接触,不会对透镜表面造成损伤,适用于透镜的高精度轮廓测量。     

光学透视测量玻璃纤维增强复合材料板离面应变场分布

本文通过激光波数扫描干涉测量系统和方法,透视测量商用玻璃纤维增强复合材料板前、后表面的离面应变场分布。实验中分别对有缺陷和无缺陷的商用玻璃纤维增强复合材料样品进行了离面位移场测量和平均轴向正应变场分布计算。无缺陷样品的离面位移场及压缩应变场分布均匀连续,随加载呈现递增压缩变化;有缺陷样品的缺陷周围的离面位移场分布变化无规则,其压缩正应变分布以缺陷孔洞为中心,加载量越大,孔洞周围其压缩正应变值较小的区域越大,压缩正应变集中在远离孔洞的边缘区域,随加载量变化压缩应变场分布无线性规律。实验结果证明,激光波数扫描干涉测量系统和方法准确可靠,它为玻璃纤维增强复合材料板的力学性能测量提供了一个新技术平台。