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Petrenko D. E. Nikolaeva A. Yu. Lazarenko V. A. Dorovatovskii P. V. Timofeev V. I. Vlaskina A. V. Korzhenevskiy D. A. Mikhailova A. G. Rakitina T. V. 《Crystallography Reports》2020,65(2):264-268
Crystallography Reports - Oligopeptidases B (OpdBs) are two-domain serine peptidases with trypsin-like substrate specificity belonging to the prolyl oligopeptidase family. These enzymes are... 相似文献
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Petrenko D. E. Nikolaeva A. Yu. Lazarenko V. A. Dorovatovskiy P. V. Timofeev V. I. Vlaskina A. V. Korzhenevskiy D. A. Mikhailova A. G. Boyko K. M. Rakitina T. V. 《Crystallography Reports》2020,65(6):909-914
Crystallography Reports - The results of the structural studies of bacterial oligopeptidase B (OpB) belonging to the least well-studied prolyl oligopeptidase family are described. The screening of... 相似文献
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Akparov V. Kh. Timofeev V. I. Korzhenevskiy D. A. Kuranova I. P. Rakitina T. V. 《Crystallography Reports》2019,64(5):750-757
Crystallography Reports - A mutant of bacterial carboxypeptidase T from Thermoactinomyces vulgaris (CPT5) with amino-acid substitutions in the S1' specificity pocket of the active site for the... 相似文献
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D. A. Altukhov Y. K. Agapova A. V. Vlaskina D. A. Korzhenevskiy A. Y. Nikolaeva A. M. Frank-Kamenetskaya E. V. Bocharov T. V. Rakitina 《Moscow University Chemistry Bulletin》2016,71(4):221-226
We created a high-performance system for expression of 13C, 15N-double-labeled recombinant НU-proteins from mycoplasms S. melliferum (HUSpm) and M. gallisepticum (HUMgal), and a purification protocol that yields protein samples suitable for structure studies by high-resolution NMR. Using these proteins we optimized the conditions of NMR experiments and obtained two-dimensional heteronuclear 1H/15N-HSQC and 1H/13С-HSQC-CT NMR spectra of free НU-proteins (HUSpm and HUMGal), and 1H/15N-HSQC spectrum of a complex between HUSpm and a double-stranded DNA (ds-DNA). The techniques we created are applicable in the studies of НU-proteins from other organisms. They allow analyzing structure and dynamics of free proteins and of their conformational changes induced by binding of various ds-DNAs or of DNA binding inhibitors. 相似文献
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