Genome Classification of Musa cultivars from Northeast India as Revealed by ITS and IRAP Markers

Genome classification of 38 banana cultivars found in northeast India was successfully carried out using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) of the internal transcribed spacer (ITS) region and inter-retrotransposon amplified polymorphism (IRAP) techniques. The RsaI digestion of the ITS region revealed the composition of A genome in 32 cultivars and B genome in 29 cultivars. With the gypsy-IRAP marker, 33 cultivars were identified to be composed of B genome. The AluI digestion of the 420-bp PCR amplification product using copia-IRAP primer resulted in the identification of the ABB genome in 17 cultivars. Overall, the data obtained from 36 cultivars using the molecular markers were in accordance with the initial classification based on morphological characters except in two cultivars. The present findings provide the reliable information on the genome classification and the status of the existing banana genetic resources from the northeastern Indian region, which could be utilized in improvement and conservation programs.     

Measurement of(n,α)and(n,2n)reaction cross sections at a neutron energy of 14.92±0.02 MeV for potassium and copper with uncertainty propagation

Experimentally measured neutron activation cross sections are presented for the ⁶⁵Cu(n,0)^62mCu,⁴¹ K(n,a)^38Cl,and ⁶⁵Cu(n.2n)⁶⁴Cu reactions with detailed uncertainty propagation.The neutron cross secions were measured at an incident energy of 14.92±0.02 MeV,and the neutrons were based on the(d,n)a fusion reaction.The ²⁷ Al(n,a)²⁴Na reaction was used as a reference reaction for the normalization of the neutron flux.The pre-calib-rated lead-shielded HPGe detector was used to detect the residues'γ-ray spetra.The data from the measured cross sections are compared to the previously measured cross sections from the EXFOR database,theoretically calculated cross sections using the TALYS and EMPIRE codes,and evaluated nuclear data.