Determination of eugenol and isoeugenol in aquaculture water by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometryEI北大核心CSCD

An analytical method was established for the determination of eugenol and isoeugenol in aquaculture water by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry UPLC-Q-TOF-MS. Water samples were derivatized with dansyl chloride. The derivatization products were separated on a ThermoAccucore C18 column100 mm×2.1 mm2.6 μµm under gradient elution condition utilizing acetonitrile and 0.1% formic acid as mobile phases. Q-TOF-MS operating in mass data using alternating collision cell energyMSEmode with positive electrospray ionization was used for the identification of analytes. The results showed that the calibration curves for eugenol and isoeugenol were linear in the range of 0.1-20 μµg/L with the correlation coefficientsrhigher than 0.995. Their recoveries in aquaculture water were 83.2%-102% and 91.8%-104%respectivelywith relative standard deviationsRSDsless than 15%. The limits of detection were 0.02 and 0.05 μµg/L and the limits of quantitation were 0.05 and 0.1 μµg/Lrespectively. The method can be applied to the determination of eugenol and isoeugenol in real water samples. © 2022, Youke Publishing Co.,Ltd. All rights reserved.