全文获取类型
收费全文 | 1498篇 |
免费 | 51篇 |
国内免费 | 6篇 |
专业分类
化学 | 1231篇 |
晶体学 | 15篇 |
力学 | 22篇 |
数学 | 85篇 |
物理学 | 202篇 |
出版年
2023年 | 7篇 |
2022年 | 12篇 |
2021年 | 16篇 |
2020年 | 23篇 |
2019年 | 25篇 |
2018年 | 20篇 |
2017年 | 16篇 |
2016年 | 36篇 |
2015年 | 34篇 |
2014年 | 36篇 |
2013年 | 78篇 |
2012年 | 80篇 |
2011年 | 101篇 |
2010年 | 51篇 |
2009年 | 50篇 |
2008年 | 96篇 |
2007年 | 111篇 |
2006年 | 105篇 |
2005年 | 97篇 |
2004年 | 89篇 |
2003年 | 71篇 |
2002年 | 58篇 |
2001年 | 36篇 |
2000年 | 17篇 |
1999年 | 17篇 |
1998年 | 17篇 |
1997年 | 16篇 |
1996年 | 11篇 |
1995年 | 16篇 |
1994年 | 16篇 |
1993年 | 13篇 |
1992年 | 9篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1988年 | 5篇 |
1987年 | 11篇 |
1986年 | 4篇 |
1985年 | 13篇 |
1984年 | 7篇 |
1983年 | 9篇 |
1982年 | 12篇 |
1981年 | 9篇 |
1980年 | 15篇 |
1979年 | 9篇 |
1978年 | 15篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1975年 | 12篇 |
1974年 | 9篇 |
1973年 | 6篇 |
排序方式: 共有1555条查询结果,搜索用时 15 毫秒
1.
Jumiarti Agus Hideki Abe Yoshiharu Doi Takeharu Tsuge 《Polymer Degradation and Stability》2006,91(8):1645-1650
Polyhydroxyalkanoate (PHA) synthase (PhaC) from Wautersia eutropha was expressed in a wide range of production level in Escherichia coli XL1-Blue cells and its effects on PhaC activity, poly[(R)-3-hydroxybutyrate] [P(3HB)] production and its molecular weights were investigated. The production level of PhaC was controlled both by the amount of chemical inducer (isopropyl-β-d-thiogalactopyranoside, IPTG) added into the medium and the use of different copy number of plasmids. In a flask experiment, as PhaC production level in the cells increased, the PhaC activity also increased in the range of low PhaC concentration. However, PhaC activity did not further increase in the range of high PhaC concentration, probably due to the formation of inclusion body in the cells. The molecular weight of P(3HB) was found to decrease with increasing PhaC activity. This trend was also verified in high cell density cultivation using 10-l jar fermentor. Furthermore, we demonstrated that the use of low copy number plasmid and appropriate induction of PhaC expression were effective in achieving both high productivity and high molecular weight of P(3HB). 相似文献
2.
3.
Yuichi Kamiya 《Acta Mathematica Hungarica》2004,105(1-2):103-114
A. Beurling introduced harmonic functions attached to measurable functions satisfying suitable conditions and defined their spectral sets. The concept of spectral sets is closely related to approximations by trigonometric polynomials. In this paper we consider spectral sets of the harmonic functions attached to the Riemann zeta-function and its modification. 相似文献
4.
Crosslinked poly(N-isopropylacrylamide) gel was tested on the feasibility for a preparative electrophoretic matrix. Horse heart myoglobin and bovine hemoglobin were well separated on the gel matrix electrophoretically by molecular sieving effect of the gel network. Relative mobilities of those proteins in the gel were larger than those in a crosslinked polyacrylamide gel of the same polymer concentration. After the separation, the protein-containing portion of the gel underwent swelling at 4°C and deswelling at 37°C, alternatively. As a result of the deswelling, each protein was recovered in a discharged solution out of the gel at almost 100% yield. 相似文献
5.
In this study, we attempted to control the timing of light-emission from bioluminescent bacteria, by changed cell numbers inoculated into medium. Luminous bacteria express bioluminescence when the number of cells reached a threshold. Inoculated cell density had an effect on the time of bioluminescence starting. Samples were prepared by varying cell density of inoculation. In the results, all the vials showed different luminescence profiles in the order of inoculated cell population. 相似文献
6.
7.
Takayuki Doi 《Tetrahedron letters》2004,45(29):5727-5729
Catch and release method utilizing polymer-support was investigated in the separation of 1α,25-(OH)2 pre- and provitamin D3. Polymer-supported alkyldiisopropylsilyl triflate selectively captured the previtamin D3 from a 26:74 mixture of pre- and provitamin D3 produced by photoisomerization of provitamin D3. 相似文献
8.
The friction coefficient between the polymer network of an opaque poly(acrylamide) gel and water is measured as a function of the mole fraction of cross linker. The friction coefficients of opaque gels are 4 to 5 orders of magnitude smaller than those of the transparent gels. This drastic decrease in friction occurs when the mole fraction of cross linker is 0.2. In opaque gels, the friction coefficient of gels and the mole fraction of cross linker are related by a power law. The network structure of the opaque gels used in the friction measurements is examined with a confocal laser scanning microscope. The opaque gel network consists of a fractal aggregate of colloidal particles. The radius of particles and the volume occupied by the particles depend on the mole fraction of cross linker. Both relationships are well described by the power laws. The power law of the friction coefficient is well explained in terms of the power laws of the structural parameters and the Stokes equation of the hydrodynamic friction for the spherical particle. It indicates that the friction of the opaque gel is determined simply by the structure of the polymer network. 相似文献
9.
A poly(N-isopropylacrylamide) (PNIPAAm)-like biodegradable thermosensitive polydepsipeptide, poly[Glc-Asn(N-isopropyl)], was synthesized by introducing an isopropyl amide group into poly[Glc-Asn]. Poly[Glc-Asn(N-isopropyl)] was degraded in vitro by cleavage of the ester bonds in the main chain in water at room temperature. The non-toxic nature of the polymer and its degradation products, coupled with a cloud point at 29 degrees C in water, make this polymer attractive for biomedical implant applications. 相似文献
10.
We achieved the total synthesis of the histone deacetylase inhibitor spiruchostatin A, as the prelude to the preparation of a combinatorial library of its analogues. Two key reactions were an asymmetric acetate aldol reaction using a Zr-enolate and macrolactonization using the Shiina method. 相似文献