Efficient transformation of 7,14-dihydroxy-ent-kaurenes to novel ent-abietanes having cis-fused α-methylene γ-lactones under Mitsunobu reaction conditions and their cytotoxicities

Transformation of plant-origin 7,14-dihydroxy-ent-kaurenes to ent-abietanes having a cis-fused α-methylene γ-lactones was accomplished efficiently under the Mitsunobu reaction conditions. The yields of the desired products were apparently influenced by the steric hindrance at C-1. The cytotoxic activity on P388 murine leukemia cells of the ent-abietanes having cis-fused α-methylene γ-lactones produced were assayed.     

离子液体均匀提取-高效液相色谱法测定参附注射液中4种单酯型乌头碱的含量

摘要 采用离子液体均匀提取-高效液相色谱法测定了参附注射液中苯甲酰乌头碱（BA）、苯甲酰新乌头碱（BM）、苯甲酰次乌头碱（BH）和苯甲酰脱氧乌头碱（BD）的含量,通过优化离子液体的用量,样品溶液的pH值,提取时间和盐浓度等参数,确定最佳提取条件为：pH为中性,盐浓度为5%,采用0.058g的[C6Mim][ BF4]和0.26g的 NH4PF6作为提取剂,提取1min,可有效地将目标物从5mL注射液中提取出来。在此实验条件下测得四种单酯型乌头碱的检测限分别为45.5（BM）,19.8(BA),26.3(BH)和12.2(BD)μg L-1,对参附注射液样品进行分析,加样回收率范围在93.74-107.91%,RSD值均小于4.34%。     

基质固相分散提取桃儿七根中鬼臼毒苷和异鬼臼苦酮

采用基质固相分散提取桃儿七根中的鬼臼毒苷和异鬼臼苦酮,并用高校液相色谱法进行了测定,选择硅胶为分散剂和甲醇为洗脱剂对鬼臼毒苷进行提取,硅藻土为分散剂和甲醇为洗脱剂对异鬼臼苦酮进行提取,与回流提取法相比,提取率较高。     

狗枣猕猴桃叶化学成分研究

从狗枣猕猴桃叶中分离得到7个黄酮类化合物, 经1D NMR, 2D NMR和MS等波谱分析, 鉴定它们的化学结构分别为山柰甲黄素-3-O-芸香糖苷(Ⅰ)、 山柰甲黄素-7-O-(4"-O-乙酰基鼠李糖基)-3-O-β-D-吡喃葡萄糖苷(Ⅱ)、山柰甲黄素-7-O-(4"-O-乙酰基鼠李糖基)-3-O-芸香糖苷(Ⅲ)、山柰酚-3-O-β-D-葡萄糖苷(Ⅳ)、山柰酚-3-O-芸香糖苷(Ⅴ)、山柰甲黄素-3-O-β-D-葡萄糖苷(Ⅵ)和山柰甲黄素-7-O-鼠李糖基-3-O-芸香糖苷(Ⅶ). 其中化合物Ⅰ, Ⅱ和Ⅲ为新化合物, Ⅳ, Ⅴ和Ⅵ为首次从该植物中分离得到.     

Determination of Ten Rare Ginsenosides in Three Kinds of Injection by SPE and HPLC

A method combining solid-phase extraction and high-performance liquid chromatography (SPE–HPLC) has been developed for analysis of ten rare ginsenosides including 20(R)-Rh₁, 20(S)-Rh₁, 20(R)-Rg₃, 20(S)-Rg₃, Rg₅, Rk₁, Rg₆, F₄, Rk₃, and Rh₄ in three kinds of injection (Shenfu, Shenmai, and Shengmai). Waters Oasis HLB SPE columns were used to clean and enrich the sample. An Eclipse XDB-C₁₈ column was used to separate the analytes, with water and acetonitrile as mobile phase components under gradient elution conditions at 30 °C. There were good linear correlations between the signals measured and the concentrations of the ten analytes (r ² ≥ 0.9996). Intraday and interday precision were both better than 2.19% and average recovery ranged from 95.25 to 108.83%. The proposed method was successfully applied to determination of the ten analytes in different batches of the injections and the results indicated the method is simple, rapid, and accurate. The proposed method could be used for quality control during manufacture of the injections.

     

狗枣猕猴桃叶化学成分研究(Ⅱ)

从狗枣猕猴桃叶中分离出4个新的黄酮类化合物, 经1D NMR, 2D NMR及ESI-MS等波谱分析鉴定了其结构, 分别是4'-甲氧基-槲皮素-7-O-(4"-O-乙酰基-鼠李糖基)-3-O-β-D-吡喃葡萄糖苷(1), 山柰甲黄素-7-O-(3"-O-乙酰基-鼠李糖基)-3-O-芸香糖苷(2), 山柰酚-7-O-(4"-O-乙酰基-鼠李糖基)-3-O-芸香糖苷(3)和4'-甲氧基-槲皮素-7-O-(4"-O-乙酰基-鼠李糖基)-3-O-芸香糖苷(4).     

Efficient synthesis of novel cytotoxic cis-fused α-methylene γ-lactones from 7,14-dihydroxy-ent-kaurenes by transformation under Mitsunobu reaction conditions

Facile transformation of 7,14-dihydroxy-ent-kaurenes such as excisanin A (8), kamebanin (9), and kamebakaurin (10), which are abundant in plants of the genus Rabdosia species (Labiatae), to ent-abietanes was accomplished under the Mitsunobu reaction conditions. The δ, ε-unsaturated cis-fused α-methylene γ-lactones (17, 18, and 25) thus prepared showed a moderate cytotoxic activity on P388 murine leukemia cells.     

Robust image encryption by combined use of integral imaging and pixel scrambling techniques

A robust image encryption method by using the integral imaging and pixel scrambling (PS) techniques is proposed. In this method, pixels of the cover image are scrambled with the PS technique and elemental images for this scrambled image are picked up through a lenslet array. Subsequently, an encrypted image is obtained by scrambling these picked-up elemental images. Since this encrypted image has the hologram-like property of data redundancy resulted from the integral imaging scheme, while it can as well be decoded by multiple keys such as the orders of pixel scrambling and the pickup conditions of the elemental images, its security against the various attacks could be dramatically improved. Good experimental results also confirm that the proposed method could provide more enhanced robustness against data loss and Gaussian noises compared to the conventional methods.     

碱催化降解法制备抗癌活性化合物20(S)-原人参二醇

通过碱催化降解制备了与植物体内结构一致且具有抗癌活性的人参皂苷元--20(S)-原人参二醇,并对其进行分离及结构表征. 将西洋参茎叶总皂苷和强碱溶于高沸点有机溶剂中,在常压和高温条件下进行降解. 通过正交试验确定了制备20(S)-原人参二醇的最佳降解条件,并将降解物经萃取、 柱层析及重结晶等方法分离得到20(S)-原人参二醇. 按西洋参茎叶总皂苷计,20(S)-原人参二醇产率为5.01%,纯度为98.56% . 通过理化性质和光谱分析可确认该化合物为20(S)-原人参二醇. 所制备的20(S)-原人参二醇具有产率和纯度高及成本低等特点.     

珠子参化学成分分析

从珠子参根茎中分离得到7个化合物. 利用核磁共振、 质谱和红外等手段, 并结合其理化性质, 鉴定了其结构, 它们分别是24(R)-珠子参苷R1, 6-O-[β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖基]-20-O-[β-D-吡喃葡萄糖基(1→4)-β-D-吡喃葡萄糖基]-20(S)-原人参三醇、 6″-乙酰基-人参皂苷Rd、 人参皂苷Rf、 竹节参皂苷Ⅳa、 人参皂苷Rd和竹节参皂苷Ⅴ. 其中, 24(R)-珠子参苷R1和6-O-[β-D-吡喃葡萄糖基(1→2)-β-D-吡喃葡萄糖基]-20-O-[β-D-吡喃葡萄糖基(1→4)-β-D-吡喃葡萄糖基]-20(S)-原人参三醇为2个新化合物, 6″-乙酰基-人参皂苷Rd 和人参皂苷Rf为首次从珠子参根茎中得到.