Determination of Diethylstilbestrol in Aquatic Products by High Performance Liquid Chromatography with Fluorescence Detection

A sensitive method for the determination of diethylstilbestrol (DES) in aquatic products by high performance liquid chromatography (HPLC) using a fluorescence detector (FLD) was developed. By means of sulfonation in concentrated sulfuric acid and hydrolysis, DES was converted into a reaction product that can emit fluorescence and determined by HPLC. For the first time, HPLC with FLD was applied to the determination of DES and sensitivity comparable to that of LC‐MS gained. The limit of detection was 0.1 µg·kg^?1, and the recoveries were above 86% with relative standard deviations less than 6.2%. The proposed method was successfully applied to the determination of DES in aquatic products.     

Indium-111 labeled bleomycin for targeting diagnosis and therapy of liver tumor: optimized preparation,biodistribution and SPECT imaging with xenograft models

In this work, the preparation of ¹¹¹In radiolabeled bleomycin (¹¹¹In-BLM) was optimized systematically and used for SPECT imaging of liver cancer xenograft models. ¹¹¹In-BLM with a high radiochemical yield (>?99%) could be obtained at pH 0.5–1 at a mixture ratio of 9:1 (¹¹¹In/BLM, mCi/mg). The radiochemical purity of ¹¹¹In-BLM retained?>?99% in either buffers or serum for 3 days. Biodistribution of ¹¹¹In-BLM revealed its excellent stability in vivo. The SPECT imaging studies showed ¹¹¹In-BLM has great specificity to liver tumor xenograft. All the results implied ¹¹¹In-BLM is a potential radiopharmaceutical for targeting diagnosis and therapy of liver tumor.