谷物种子中蛋白质含量测定的核反应分析法

分析了在高蛋白质谷物育种中对筛选方法的要求, 对谷物中蛋白质测定的方法进行了综述评价, 重点介绍了用于谷物蛋白质测定的核反应分析法的原理及特点. The requirements of the screening method for high protein content in grain breeding are analysed. Methods for the determination of protein content in grain seed are reviewed.The principle and the advantages of the nuclear reaction method for the measurement of protein content in grain seed are discussed in detail.     

Comparative Reactivity of Phosphate Ester Hydrolysis Catalyzed by Mononuclear and Hetero-Dinuclear Complexes Containing the Lanthanum Ion (III)

The hydrolysis of bis(p-nitrophenyl phosphate)(BNPP) and p-nitrophenyl phosphate (NPP) were strongly promoted by the mononuclear La^III[(N,N-bis(3-carboxyl)-2-hydroxysalicylidene)ethylenediamine] and the hetero-dinuclear Cu^IILa^III[(N,N-bis(3-carboxyl)-2-hydroxysalicylidene)ethylenediamine] complexes at 35 °C in 50% aqueous DMSO. The results indicated that the hetero-dinuclear Cu^IILa^III complex exhibited a relatively higher catalytic function on NPP hydrolysis, while the hydrolysis of BNPP was slightly more efficient in the presence of the mononuclear La^III complex than that in the presence of the Cu^IILa^III complex, and this case was discussed by the different spacer requirements for NPP and BNPP hydrolysis. In addition, widely differing pH-rate profiles were obtained: saturated curves for NPP hydrolysis and bell-shaped curves for BNPP hydrolysis, and thus different kinetic treatments were employed to obtain the relative parameters.