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Volkov V. A. Voronkov M. V. Sazhina N. N. Kurilov D. V. Vokhmyanina D. V. Yamskova O. V. Martirosyan Yu. Ts. Atroshenko D. L. Martirosyan L. Yu. Romanova V. S. 《Kinetics and Catalysis》2021,62(3):395-403
Kinetics and Catalysis - The kinetic characteristics, mechanisms of activity, and relationship between the antioxidant activity and the molecular and supramolecular structure of fullerene C60 and... 相似文献
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Yamskov I. A. Blagodatskikh I. V. Krasnov M. S. Borisenko A. V. Margasyuk D. V. Vecherkin V. V. Skripnikova V. S. Nazarova P. A. Bitko S. A. Berezin B. B. Yaminskii I. V. Meshkov G. B. Grachev S. A. Serebryakova M. V. Rybakova E. Yu. Yamskova V. P. 《Russian Chemical Bulletin》2009,58(3):640-645
Russian Chemical Bulletin - The found similarity of the set of physicochemical characteristics of regulatory proteins active in microdoses isolated from various mammal tissues allows them to be... 相似文献
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A. S. Yasnaya O. V. Yamskova D. T. Guranda T. A. Shcherbakova V. I. Tishkov V. K. Švedas 《Moscow University Chemistry Bulletin》2008,63(2):103-107
The gene of penicillin acylase (PA) from Escherichia coli has been cloned from a PA producer strain that is an analogue of strain ATCC 11105. Optimization of the cultivation conditions made it possible to obtain up to 130 mg of active enzyme per liter of culture broth. A number of single, double, and triple mutants were obtained by the method of site-specific mutagenesis using PCR. As a result of isolation and purification procedures, homogeneous preparations of the wild-type enzyme and its mutants were obtained. Studies showed that (1) the obtained enzymes have the correctly folded structure; (2) complexing agents and metal cations do not inhibit their catalytic activity; (3) mutant PAs, like the wild type, are efficiently inactivated by phenylmethylsulfonyl fluoride (PMSF), which makes it possible to titrate their active sites; and (4) the obtained mutants are characterized by a greater specificity constant in the reaction of hydrolysis of a colorimetric substrate; however, they are inferior to the wild type in the synthesis of ampicillin by acyl transfer. 相似文献
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