Effects of ultrasonic field on structure evolution of Ni film electrodeposited by bubble template method for hydrogen evolution electrocatalysis

Journal of Solid State Electrochemistry - Self-supporting porous Ni film with uniform honeycomb-like micropores and a thickness of up to 66 μm is electrodeposited by dynamic hydrogen...     

Determination of 20(S)-protopanaxadiol in rat plasma by LC-MS/MS and its application to the pharmacokinetic study: A comparative study of its solution and two oral formulations

A high-performance liquid chromatography — tandem mass spectrometry method was used in a comparative pharmacokinetic study on 20(S)-protopanaxadiol in its solution, pharmacosomes and hydroxy-propyl-β-cyclodextrin inclusion compound. The calibration linearity range was 2.5–5000 ng/mL and the limit of quantification was 2.5 ng/mL. The intra- and inter-assay coefficients of variation were less than 7% and the recoveries were 67.4, 63.3 and 69.6% at 5, 250 and 4500 ng/mL. In contrast to 20(S)-protopanaxadiol solution, its pharmacosomes and hydroxypropyl-β-cyclodextrin inclusion compound improved the oral bioavailability to different extent and meanwhile were much safer in clinical use.     

Research on the resolution of micro stereo lithography

Micro stereo lithography is a kind of technology utilizing the solidified effect that photo curable polymer will appear under ultraviolet （UV） laser exposure. It is widely used in three-dimensional （3D） micro fabrication. We get the experimental values of a pair of UV laser curing coefficients, absorption coefficient and critical curing energy, of curable resin by fitting the calculation results of the Gaussian beam theory and experimental curing results. The theoretical relation between the curing unit＇s shape and the exposure features of time and intensity of convergent Gaussian beam is presented. The calculation and experimental results of curing unit under different conditions agree well with each other. This research offers a steady base for further research about the improvement of resolution.     

Fabrication of silica nanoparticles and hollow spheres using ionic liquid microemulsion droplets as templates

Silica products with two different morphologies were synthesized using nonaqueous ionic liquid microemulsion droplets as templates. The morphologies of the obtained products were characterized by both transmission electron microscopy (TEM) and scanning electron microscopy (SEM). By adjusting the reaction conditions, ellipsoidal nanoparticles were formed under acidic conditions, while hollow silica spheres were obtained under alkaline conditions. It is demonstrated that the size distribution of hollow silica spheres was narrower than that of the ellipsoidal nanoparticles. The various vibration modes of different functional groups in the silica materials were revealed by Fourier transform infrared (FTIR) spectroscopy. The two samples were both shown to be amorphous, not crystalline by X-ray diffraction (XRD). A simple diagram of the formation process including the hydrolysis and condensation reactions is given. Furthermore, a probable mechanism for the formation of silica materials under acidic or alkaline conditions is presented, which may be helpful for better understanding the different silica materials obtained under different conditions.     

Investigation on the oxidation of aryl oxiranylmethanols and the synthesis of 2-aryl-N-thiazolyl-oxirane-2-carboxamides as glucokinase activators

RuCl₃/NaIO₄-initiated oxidation of oxiranemethanols was investigated, and two products, oxirane-2-carboxylic acid and (5′-oxotetrahydrofuran-3-yl)acetic acid, were obtained in variant ratios. The product structures were determined and a tentative mechanism involving oxidation-rearrangement-oxidation process was proposed. Glucokinase enzymatic assay revealed that oxiranecarboxamides 4a-c retained moderate GK activation potency with amide 4a showing an EC₅₀ value of 584 nM and a high activation fold of 3.14. However, (5′-oxotetrahydrofuran-3-yl)acetamide 11a is inactive. This study not only provided an alternative protocol to access (5′-oxotetrahydrofuran-3-yl)acetic acid analogs, but also yielded nanomolar GA activators (esp. 4a) for further structure-activity relationship study.     

反相高效液相色谱法测定人肌腱中的胶原蛋白

建立了测定人肌腱中胶原蛋白含量的高效液相色谱法。动物肌腱中的胶原蛋白经酸水解后生成包括羟脯氨酸在内的氨基酸混合物,用2,4-二硝基氯苯对水解产物衍生化,然后以0.01 mol/L乙酸钠-乙酸缓冲液(pH 6.0)-乙腈(体积比为80∶20)为流动相,经反相C18柱分离,紫外检测器于360 nm波长处检测来测定羟脯氨酸的含量。羟脯氨酸是胶原蛋白的特异性氨基酸且含量稳定,因而可通过样品中羟脯氨酸的含量来计算胶原蛋白的含量。该方法对羟脯氨酸的检出限为3 μg/L,羟脯氨酸为3 μg/L~100 mg/L时与峰面积的线性关系良好;样品测定的相对标准偏差为1.95%,加标回收率为98.4%~110.8%。对60份人肌腱样品中胶原蛋白的含量进行了测定。所建立的方法灵敏、准确、干扰少,适用于肌腱中胶原蛋白含量的测定。     

Optimization of Naringenin Nanoparticles to Improve the Antitussive Effects on Post-Infectious Cough

Naringenin (NRG) is a natural compound with several biological activities; however, its bioavailability is limited owing to poor aqueous solubility. In this study, NRG nanoparticles (NPs) were prepared using the wet media milling method. To obtain NRG NPs with a small particle size and high drug-loading content, the preparation conditions, including stirring time, temperature, stirring speed, and milling media amount, were optimized. The NRG (30 mg) and D-α-tocopherol polyethylene glycol succinate (10 mg) were wet-milled in deionized water (2 mL) with 10 g of zirconia beads via stirring at 50 °C for 2 h at a stirring speed of 300 rpm. As a result, the NRG NPs, with sheet-like morphology and a diameter of approximately 182.2 nm, were successfully prepared. The NRG NPs were stable in the gastrointestinal system and were released effectively after entering the blood circulation. In vivo experiments indicated that the NRG NPs have good antitussive effects. The cough inhibition rate after the administration of the NRG NPs was 66.7%, cough frequency was three times lower, and the potential period was 1.8 times longer than that in the blank model group. In addition, the enzyme biomarkers and histological analysis results revealed that the NRG NPs can effectively regulate the inflammatory and oxidative stress response. In conclusion, the NRG NPs exhibited good oral bioavailability and promoted antitussive and anti-inflammatory effects.     

稀土离子(La3+,Ce3+,Tb3+,Y3+)对炎症及血小板聚集的影响

研究稀土离子(La3 ,Ce3 ,Tb3 ,Y3 )对炎症、血小板聚集及蛋白磷酸化的影响.采用二甲苯使小鼠耳部致炎,腹腔注射稀土离子,观察炎症的变化;利用血小板聚集仪观察稀土离子对血小板聚集的影响;用放射标记法测量稀土对血小板蛋白磷酸化程度的影响.结果表明,稀土离子在2.5×10-4mol·L-1·kg-1的注射剂量下,能显著加强炎症反应;1×10-3mol·L-1的轻稀土(La3 ,Ce3 )对由ADP诱导的血小板聚集有明显的抑制作用,而重稀土(Tb3 ,Y3 )有明显的促进作用;浓度在1×10-6～1×10-4mol·L-1时,轻、重稀土均可促进血小板蛋白磷酸化.稀土离子对炎症、血小板聚集及蛋白磷酸化的影响与稀土的种类和剂量有关.     

Drug-Induced Phase Transition from Cubic Phase to Vesicle

The effect of caffeine on the phase behavior of aqueous dispersion of cubic phase was studied. The morphologies of the dispersed particles were observed by means of cryo-transmission electron microscopy (TEM) and the sizes of the dispersed particles were determined by dynamic light scattering (DLS). It was found that the addition of caffeine could lead to the phase transition from cubic phase to vesicle, and this might be due to the “salt out” effect of caffeine on the monoglyceride molecules in aqueous phase.     

The electrodynamics of rod-like microparticles based on optically induced dielectrophoresis

Due to its characteristics of noncontact, non-damage, high flux, and easy-to-achieve flexible manipulation, optically induced dielectrophoresis (ODEP) technology has been employed to manipulate microspherical biological particles, including separation, enrichment, capture, arrangement, and fusion. However, in nature, biomolecules are morphologically diverse, and some of them are rodlike. In order to illustrate the electrodynamics of rodlike particles under the action of ODEP, a transient multi-physical field coupling model of ODEP chip under the hypothesis of electrical double layer thin layer was established in this paper. The arbitrary Lagrangian–Eulerian method is used to track single-rod particle in the strong coupled flow field and electric field simultaneously. The influence of several key factors, including the applied alternating current (AC) electric voltage, the width of optical bright area, and the initial position of particle, on the trajectory of particle center was analyzed in positive dielectrophoresis (DEP) action and negative DEP action, respectively. Especially, the planar motion process of rodlike particles was discussed together. The research results reveal the electrodynamics behavior of rodlike particles based on the action of ODEP, which may provide theoretical support for the further design of rodlike biological cells manipulation chip based on AC ODEP technology in the future.