Phase Diagram for the RbBr–CuBr System

The phase diagram for the RbBr–CuBr system has been determined. In the system two intermediate compounds are formed: RbCu₂Br₃, melting congruently at 537 K and Rb₃CuBr₄, melting incongruently at 544 K. The coordinates of the two eutectic points are: 501 K, 54 mole% CuBr and 522 K, 74 mole% CuBr. This revised version was published online in July 2006 with corrections to the Cover Date.     

Phase equilibrium diagram for the tin(II) iodide-copper(I) iodide system

The phase diagram of the SnI₂-CuI system was determined by the method of thermal analysis. No intermediates were found. It is suggested that limiting solid solutions of SnI₂ in-CuI,-CuI and-CuI, respectively, are formed. The eutectic point parameters (32.7 mol% CuI, 270.4 ±0.1 °C) and those of two invariant points (64.0 mol% CuI, 385.3 ±0.5 °C and 61.5 mol% CuI, 366.6±0.2 °C), corresponding to the equilibria+liquid and+liquid , respectively, were determined.Mittels Thermoanalyse wurde das Phasendiagramm SnI₂-CuI bestimmt. Es konnten keine Intermediäre gefunden werden. Es scheint, daß Grenzmischkristalle von Shl2 und-CuI,-CuI,-CuI gebildet werden. Es wurden ein Eutektikum (32,7 mol% CuI, 270,4 ± 0,1 °C) und zwei den Gleichgewichten+Flüssigkeit und+Flüssigkeit entsprechende invariante Punkte (64,0 mol% CuI, 385,3±0,5 °C und 61,5 mol% CuI, 366,6±0,2 °C) gefunden. SnI₂-CuI, . , , -CuI,-CuI Cul. (32,7 % CuI, 270,4±0,1°) (64,0 % Cul, 385,3 ±0,5° 61,5 % CuI, 366,6±0,2°), + + .

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The present work was carried out within the framework of contract CPBP 01.15.     

Phase diagram and dissolution studies of the fenofibrate–acetylsalicylic acid system

Enhancement of the dissolution rate of poorly soluble compounds through the formation of drug–drug eutectics was investigated using fenofibrate and acetylsalicylic acid. Solid–liquid equilibria in the system under study were investigated by differential scanning calorimetry (DSC). The phase diagram for the whole range of compositions was constructed. In addition, existence of a metastable polymorph of fenofibrate has been confirmed. The investigation has revealed that acetylsalicylic acid and fenofibrate form a simple eutectic mixture containing 0.958 mol fraction of fenofibrate at the eutectic point. Dissolution rate improvement of fenofibrate correlated with the phase diagram. The amount of fenofibrate released from the solid dispersions that contained fenofibrate as the eutectic mixture with acetylsalicylic acid was at least threefold higher compared to untreated fenofibrate.     

Melting and high-temperature solid state transitions in cobalt(II) halides

Melting and high temperature solid-state transitions in CoCl₂ and CoBr₂ are widely discussed. On the basis of DSC and conductometric measurements it was found that melting process of CoCl₂ is preceded by a solid-state transition appearing about 20 K below the melting point of CoCl₂. Due to deconvolution of the thermograms, the enthalpy of fusion and that of solid-state transition were found to be 36.4 and 9.6 kJ mol^–1, respectively. Melting points of CoCl₂ and CoBr₂ were established to be 999.0 and 949.7 K, respectively. Hitherto unknown enthalpy of fusion of CoBr₂ was determined to be 27.2 kJ mol^–1. A solid-state transition in CoBr₂ at 650 K has been confirmed.     

Diagrammes d'equilibre des systemes d'halogenures CuY-MYn

Phase diagrams for the series of CuY-M^IY and CuY-M^IIY systems (whereM ^I=Li, Na, K, Rb, Cs, Tl,M ^II=Zn, Cd, Hg, Sn, Pb andY=Cl, Br, I) were compared. Changes and trends accompanying the increase of charge of the second cation were discussed.

     

Phase equilibrium diagram for the tin(II) bromide-copper(I) bromide system

The phase diagram of the SnBr₂-CuBr system was determined by the method of thermal analysis. One intermediate compound, CuSn₅Br₁₁, melting congruently at 218.7 °C, was found.     

An optimized method to extract poplar leaf proteins for two‐dimensional gel electrophoresis guided by analysis of polysaccharides and phenolic compounds

Commonly used methods for protein extraction from plant leaves, such as extraction with phenol or a combination of trichloroacetic acid and acetone, were ineffective for four tested cultivars of poplar. Moreover, multiple protocols for 2DE of the extracted proteins gave different results when protein profiles of relatively closely related plants were compared. Given that polycyclic compounds strongly hinder 2DE, we analyzed the impact of polyphenols and polysaccharides present in the plant tissues used for protein extraction, on the quality of 2DE protein profiles. Analysis of content of polyphenols and polysaccharides in leaves of poplar cultivars showed that even small differences in concentrations of analyzed metabolites accompany large differences between poplar cultivars when considering the susceptibility of samples to protein extraction for 2DE. High‐quality 2DE results were correlated with decreased amounts of polyphenols. Additional analysis using MS/MS suggested that only levels of total phenolics affected the results of 2DE. Soluble total nonstructural carbohydrates also had a negative effect, but the level of starch was not important. Finally, we present an optimized method for extraction of proteins from poplar leaves, which enables reliable comparative analysis of four different poplar cultivars, that is, “Eridano,” “Villafranca,” “NE‐42,” and “Luisa Avanzo,” which have not yet been used for the proteomic studies.     

Structural characterization of flavonoid glycosides from leaves of wheat (Triticum aestivum L.) using LC/MS/MS profiling of the target compounds

The aim of this study was to present integrated mass spectrometric methods for the structural characterization and identification of flavonoid glycoconjugates. During the liquid chromatography/mass spectrometry analyses, TriVersa NanoMate chip‐based system with nanoelectrospray ionization and fraction collection was combined to a quadrupole time‐of‐flight mass spectrometer. In the extract samples prepared from green leaves of wheat plantlets, 41 flavonoid derivatives were recognized. Part of the target natural products had the full structure being characterized after the registration of mass spectra, where m/z values for protonated [M + H]⁺ and deprotonated molecules [M ? H]^? were annotated. MS² and pseudo‐MS³ experiments were performed for [M + H]⁺ or [M ? H]^? and aglycone ions (Y₀^+/?‐type), respectively. It should be underlined that pseudo‐MS³ mass spectra were registered for aglycone product ions in the mass spectra of O‐glycosides present in the extract samples. In many cases, only tentative structural identification of aglycones was possible, mainly because of the presence of numerous C‐monoglycoside or C‐diglycoside in the samples. Acylation of the sugar moiety and/or methylation of the aglycone in the flavonoid glycosides under study was observed. The existence of isobaric and/or isomeric compounds was demonstrated in the extract studied. The collision‐induced dissociation mass spectra registered for C,O‐diglycosides and C,C‐diglycosides did not permit to draw complete structural conclusions about the compounds studied. For the investigated class of natural products, unambiguous classification of sugar moieties linked to the aglycones from the recorded mass spectra was not possible. Registration of the positive and negative ion mass spectra did not lead to the precise conclusion about the glycosylation position at C‐6 or C‐8, and O‐4′ or O‐7 atoms. It was possible, on the basis of the collected MS² spectra, to differentiate between O‐glycosides and C‐glycosides present in the samples analyzed. Copyright © 2013 John Wiley & Sons, Ltd.     

Studies of phase equilibria in the systems ZnCl2-AgCl and ZnBr2-AgBr

Phase equilibria in the systems zinc chloride - silver chloride and zinc bromide - silver bromide were investigated by differential scanning calorimetry, electric conductivity measurements and X-ray diffraction methods. No intermediate compounds were found. Solubility of components in the solid-state is negligible. Both systems are of the eutectic type. The eutectic temperatures in the ZnCl₂-AgCl system and in the ZnBr₂-AgBr system are 504 K and 551 K, respectively, and the compositions of the eutectic point are 53.5 mol% AgCl and 59.4 mol% AgBr, respectively. This revised version was published online in July 2006 with corrections to the Cover Date.