Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening

Background  

The mitotic exit network (MEN) is a group of proteins that form a signaling cascade that is essential for cells to exit mitosis in Saccharomyces cerevisiae. The MEN has also been implicated in playing a role in cytokinesis. Two components of this signaling pathway are the protein kinase Dbf2 and its binding partner essential for its kinase activity, Mob1. The components of MEN that act upstream of Dbf2-Mob1 have been characterized, but physiological substrates for Dbf2-Mob1 have yet to be identified.     

Steady streaming around a spherical drop displaced from the velocity antinode in an acoustic levitation field

The steady (acoustic) streaming associated with a sphericaldrop displaced from the velocity antinode of a standing waveis studied. The ratio of the particle size to the acoustic wavelengthis treated as small but non-zero, and the solution is developedin the form of a two-term expansion in terms of the correspondingsmallness parameter. The drop viscosity is assumed to be muchhigher than that of the surrounding fluid, which is the casefor a drop in a gas medium. There are essentially three distinctregions where the steady streaming flow is analysed: insidethe drop (internal circulation), in the Stokes shear-wave layerat the surface on the gas side, and the gas outside the Stokeslayer (the outer streaming region). Solutions for the internalcirculation and the outer streaming are obtained in the limitof small Reynolds number. Despite the gas-to-liquid viscosity ratio being small, the outerstreaming may be dramatically affected by the fact that thesphere is liquid as opposed to solid. The parameter that measuresthe effect of liquidity is essentially the viscosity ratio dividedby the relative (to the particle size) thickness of the Stokeslayer. The case of a solid sphere is recovered by letting thisparameter go to zero.     

Molecular logic gates using surface-enhanced Raman-scattered light

A voltage-activated molecular-plasmonics device was created to demonstrate molecular logic based on resonant surface-enhanced Raman scattering (SERS). SERS output was achieved by a combination of chromophore-plasmon coupling and surface adsorption at the interface between a solution and a gold nanodisc array. The chromophore was created by the self-assembly of a supramolecular complex with a redox-active guest molecule. The guest was reversibly oxidized at the gold surface to the +1 and +2 oxidation states, revealing spectra that were reproduced by calculations. State-specific SERS features enabled the demonstration of a multigate logic device with electronic input and optical output.     

Ueber die Bestimmung des Cadmiums

Ohne Zusammenfassung