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1.
Fred. A. Mellon Keith W. Waldron Robbie R. Selvendran Guido C. Galletti 《Journal of mass spectrometry : JMS》1994,29(10):556-560
Sclerenchyma tissues from semi-mature and mature asparagus spears were analysed by pyrolysis/gas chromatography/mass spectrometry and by conventional chemical methods in order to determine the degree of lignification and compare analytical techniques. The pyrolysis data are similar to those obtained by a chemical method involving alkaline extraction at 70°C followed by spectrophotometric determination of phenolic content. The pyrolysis method was more rapid, sensitive and informative than the chemical technique and shows considerable potential for studying the chemical basis of the textural development of plant foods. 相似文献
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3.
Peptide mapping has been widely used for the identification of modified proteins involved in certain diseases. Despite the fact that capillary electrophoresis (CE) has been shown to be a powerful tool for the separation and detection of tryptic peptide fragments after protein digestion, this technique lacks sensitivity for mapping proteins isolated in very small quantities from biological samples. Consequently, it has been necessary to preconcentrate the protein before adding the proteolytic enzyme for digestion in solution. These experimental steps are quite long, labor intensive and require a lot of sample handling. In this paper, we describe an on-line system allowing digestion of the protein, followed by preconcentration, separation and detection of the tryptic fragments in 4 h. Up to an 800-fold preconcentration factor was achieved for cytochrome c, despite a loss of separation efficiency induced by the multiple-valve design of the system and dispersion of the 60-nl desorption plug. Moreover, our system showed good migration time reproducibility between peptide maps and could be reused for several samples. 相似文献
4.
Donnelly T Waldron S Tait A Dougans J Bearhop S 《Rapid communications in mass spectrometry : RCM》2001,15(15):1297-1303
This paper describes the application of a simple chromium reduction furnace which can be interfaced with a dual inlet isotope-ratio mass spectrometer thus providing the capacity for cheap, fast, accurate and precise measurement of deltaD(V-SMOW) by dynamic mass spectrometry. Measurements are precise to the order of < or =0.5 per thousand. Mean 95% confidence intervals for the Vienna Standard Mean Ocean Water (V-SMOW) to Standard Light Antarctic Precipitation (SLAP) range are in the order of 2.5 per thousand and the system is linear over the range -428 to 23,000 per thousand. Memory effects do exist, but are small for natural abundance samples and can be minimised by careful planning of the analytical load. 相似文献
5.
Carl A. Batt Anna M. Waldron Natalie Broadwater 《Journal of nanoparticle research》2008,10(7):1141-1148
Nanotechnology will be an increasing part of the everyday lives of most people in the world. There is a general recognition
that few people understand the implications of the technology, the technology itself or even the definition of the word. This
lack of understanding stems from a lack of knowledge about science in general but more specifically difficulty in grasping
the size scale and symbolism of nanotechnology. A potential key to informing the general public is establishing the ability
to comprehend the scale of nanotechnology. Transitioning from the macro to the nanoscale seems to require an ability to comprehend
scales of one-billion. Scaling is a skill not common in most individuals and tests of their ability to extrapolate size based
upon scaling a common object demonstrates that most individuals cannot scale to the extent needed to make the transition to
nanoscale. Symbolism is another important vehicle to providing the general public with a basis to understand the concepts
of nanotechnology. With increasing age, individuals are able to draw representations of atomic scale objects, but these tend
to be iconic and the different representations not easily translated. Ball and stick models are most recognized by the public,
which provides an opportunity to present not only useful symbolism but also a reference point for the atomic scale. 相似文献
6.
Georgiana Roman-Gusetu Karen C. Waldron Dominic Rochefort 《Journal of chromatography. A》2009,1216(47):8270-8276
Microencapsulation is used here as a new technique to immobilize enzymes in a microreactor coupled off-line to capillary electrophoresis (CE), allowing the determination of enzymatic reaction products. The redox enzyme laccase was encapsulated using the method of interfacial cross-linking of poly(ethyleneimine) (PEI). The 50 μm diameter capsules were slurry packed from a suspension into a capillary-sized reactor made easily and quickly from a short length of 530 μm diameter fused-silica tubing. The volume of the bed of laccase microcapsules in the microreactor was in the order of 1.1 μL through which 50 μL of the substrate o-phenylenediamine (OPD) was flowed. The oxidation product 2,3-diaminophenazine (DAP) and the remaining OPD were quantified by CE in a pH 2.5 phosphate buffer. Peak migration time reproducibility was in the order of 0.4% RSD and peak area reproducibility was less than 1.7% RSD within the same day. Using the OPD peak area calibration curve, a conversion efficiency of 48% was achieved for a 2-min oxidation reaction in the microreactor. 相似文献
7.
Capillary zone electrophoresis is employed for the separation and analysis of both fluorescein thiohydantoin and dimethylaminoazobenzene thiohydantoin derivatives of amino acids. Detection of minute amounts of these amino acid derivatives is an important milestone in the development of a high sensitivity protein sequencer. Current detection limits for the fluorescein derivative is on the order of 10(-21) moles whereas detection limits for the dimethylaminoazobenzene derivative is on the order of 10(-16) moles. 相似文献
8.
Anna M. Waldron Carl A. Batt Clarissa S. Lui 《Journal of nanoparticle research》2011,13(12):6291-6295
Public engagement in nanotechnology media products can lead to a greater interest in understanding of nanotechnology. A study
was undertaken to determine middle school student engagement in Nanooze, a magazine featuring nanotechnology research that has been developed for a young adult audience. Teachers at 116 Detroit
middle schools distributed two issues of the magazine to their students, and surveys were collected from 870 students after
reading the magazines. Results suggest that the majority of students liked reading the magazine and learned something about
nanotechnology. Engagement in nanotechnology led to understanding of nanotechnology. The Nanooze magazine was an effective medium for engaging middle school students in learning about nanotechnology. 相似文献
9.
Shayne Waldron 《Numerical Algorithms》2006,42(2):171-179
We give an algorithm for computing orthogonal polynomials over triangular domains in Bernstein–Bézier form which uses only the operator of degree raising and its adjoint. This completely avoids the need to choose an orthogonal basis (or tight frame) for the orthogonal polynomials of a given degree, and hence the difficulties inherent in that approach. The results are valid for Jacobi polynomials on a simplex, and show the close relationship between the Bernstein form of Jacobi polynomials, Hahn polynomials and degree raising. 相似文献
10.
A screening for thermophilic cellulolytic microbes from the soils of volcanic areas yielded several potentially useful bacteria.
Of these,Microbispora bispora was the most useful. The enzyme complex was stable at 60–65‡C. Analysis of the complex indicated the presence of endoglucanase,
cellobiohydrolase, and Β-glucosidase components. The two former enzymes were secreted, whereas the Β-glucosidase was cell-associated.
The cellobiohydrolase was of particular interest, since this type of enzyme has rarely been reported from bacteria, and therefore
was characterized in greater detail. Its mechanism of action was clarified through its action pattern towards soluble (and
reduced) oligosaccharides. Synergism was observed between the cellobiohydrolase and endoglucanases during attack on crystalline
cellulose (cotton and Avicel). This represents one of the first demonstrations of synergism in a procaryotic cellulase system. 相似文献