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Liu Kang Chua Hong Lo Wai-hung Lawford Hugh Yu Peter Hoi-Fu 《Applied biochemistry and biotechnology》2002,98(1-9):1061-1073
Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. IT is one of the filamentous bacteria
causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment
could not only provide the metabolic knowledge of the strains that would be useful in the development of wastewater treatment
methods, but also could enable us to gain an understanding of the mechanism by which poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
(poly[3-HB-co-3-HV]) is produced by this strain. This article reports the screening and isolation of the strain from the activated
sludge using the Nile blue staining method together with Fourier transform infrared analysis. We investigated the ability
of the selected strain to produce poly(3-HB-co-3-HV) copolymer using glucose and peptone, or by adding valeric acid or sodium
propionate as precursor. Proper precursor feeding could dramatically enhance its 3HV content in the copolymer P(3HB-co-3HV).
By controlling the different feeding times in fed-batch fermentation, different desired copolymers were obtained with 15,
40, and 70% 3HV mole fraction of the copolymer. Polymer properties were analyzed by gas chromatography, differential scanning
calorimetry, thermo-gravimetry, and nuclear magnetic resonance analysis.
Open laboratory of Chirotechnology of the Institute of Molecular Technology for Drug Discovery and Synthesis
The University Grants Committee Area of Excellence Scheme (Hong Kong). 相似文献
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