Mapping of possible prion protein self-interaction domains using peptide arrays

Background  

The common event in transmissible spongiform encephalopathies (TSEs) or prion diseases is the conversion of host-encoded protease sensitive cellular prion protein (PrP^C) into strain dependent isoforms of scrapie associated protease resistant isoform (PrP^Sc) of prion protein (PrP). These processes are determined by similarities as well as strain dependent variations in the PrP structure. Selective self-interaction between PrP molecules is the most probable basis for initiation of these processes, potentially influenced by chaperone molecules, however the mechanisms behind these processes are far from understood. We previously determined that polymorphisms do not affect initial PrP^C to PrP^Sc binding but rather modulate a subsequent step in the conversion process. Determining possible sites of self-interaction could elucidate which amino acid(s) or amino acid sequences contribute to binding and further conversion into other isoforms. To this end, ovine – and bovine PrP peptide-arrays consisting of 15-mer overlapping peptides were probed with recombinant sheep PrP^C fused to maltose binding protein (MBP-PrP).     

Rheological behavior and structural interpretation of waxy crude oil gels

A waxy crude oil which gels below a threshold temperature has been investigated under static and dynamic conditions, using a combination of rheological methods, optical microscopy, and DSC. Particular attention is given in this work to the influence of the mechanical history on gel strength and to describing the time-dependent rheological behavior. The gels display a strong dependence of the yield stress and moduli on the shear history, cooling rate, and stress loading rate. Of particular interest is the partial recovery of the gel structure after application of small stress or strain (much smaller than the critical values needed for flow onset) during cooling, which can be used to reduce the ultimate strength of the crude oil gel formed below the pour point. A second focus of this study is to further develop the physical interpretation of the mechanism by which wax crystallization produces gelation. Gelation of the waxy crude oil studied is suggested to be the result of the association between wax crystals, which produces an extended network structure, and it is shown that the system displays features common to attractive colloidal gels, for one of which, fumed silica (Aerosil 200) in paraffin oil, rheological data are reported. The colloidal gel model provides a simple and economical basis for explaining the response of the gelled oil to various mechanical perturbations and constitutes a fruitful basis from which to develop technologies for controlling the gelation phenomenon, as suggested by the rheological results reported.     

Carathéodory balls and norm balls in a class of convex bounded Reinhardt domains

A family of the spherical fractional integrals on the unit sphere Σ _n in ℝ ⁿ⁺¹ is investigated. This family includes the spherical Radon transform (α = 0) and the Blaschke-Levy representation (α>1). Explicit inversion formulas and a characterization ofT ^αƒ are obtained for ƒ belonging to the spacesC ^∞,C, L^p and for the case when ƒ is replaced by a finite Borel measure. All admissiblen ≥ 2,α ε ℂ, andp are considered. As a tool we use spherical wavelet transforms associated withT ^α. Wavelet type representations are obtained forT ^α ƒ, ƒ εL ^p, in the case Reα ≤ 0, provided thatT ^α is a linear bounded operator inL ^p. Partially supported by the Edmund Landau Center for Research in Mathematical Analysis, sponsored by the Minerva Foundation (Germany).     

Maxwell’s Equations with Vector Hysteresis

Electromagnetic processes in magnetic materials are described by Maxwells equations. In ferrimagnetic insulators, assuming that D = E, we have the equationIn ferromagnetic metals, neglecting displacement currents and assuming Ohms law, we instead getAlternatively, under quasi-stationary conditions, for either material we can also deal with the magnetostatic equations:(Here f_ext and J_ext are prescribed time-dependent fields.) In any of these settings, the dependence of M on H is represented by a constitutive law accounting for hysteresis: M= (H), being a vector extension of the relay model. This is characterized by a rectangular hysteresis loop in a prescribed x-dependent direction, and accounts for high anisotropy and nonhomogeneity. The discontinuity in this constitutive relation corresponds to the possible occurrence of free boundaries.Weak formulations are provided for Cauchy problems associated with the above equations; existence of a solution is proved via approximation by time-discretization, derivation of energy-type estimates, and passage to the limit. An analogous representation is given for hysteresis in the dependence of P on E in ferroelectric materials. A model accounting for coupled ferrimagnetic and ferroelectric hysteresis is considered, too.Acknowledgement This research was partly supported by the project Free boundary problems in applied sciences of Italian M.I.U.R.. I gratefully acknowledge the useful suggestions from the reviewers.     

Homogenization of a parabolic model of ferromagnetism

This work deals with the homogenization of hysteresis-free processes in ferromagnetic composites. A degenerate, quasilinear, parabolic equation is derived by coupling the Maxwell-Ohm system without displacement current with a nonlinear constitutive law:

     

Studies on photoinduced effects in pulse-electrodeposited Ag/Hg-1212/CdSe hetero-nanostructures

Metal/superconductor/semiconductor (Ag/Hg-1212/CdSe) hetero-nanostructures have been fabricated using pulse-electrodeposition technique and are characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy (SEM) studies. The junction capacitance of Ag/Hg-1212, Hg-1212/CdSe and Ag/Hg-1212/CdSe heterojunctions is measured in dark and under laser irradiation at room temperature. The nature of the junction formed and built-in-junction potentials were determined. The increase in carrier concentration across the junction due to photo-irradiation has been observed.     

Activation process in hydrogen storage related to oxide layers formed on zirconium alloys

The passive behavior of ZrNi alloys near the rest potential is studied through in situ voltammetry, ellipsometry, and microscopic observation. A significant oxide layer growth is observed in aqueous 1 M KOH during the application of different potential programs currently used in the activation processes of the alloy. Oxide barrier effects and occlusion of hydrogen species within the film take place. The kinetics of the oxide layer formation under potential cycling plays a significant role in the activation process of metal alloys used in metal hydride batteries.Dedicated to Professor Gyorgy Horanyi on the occasion of his 70th birthday.     

Effect of laser irradiation of C-V characteristics of electrodeposited Ag/Tl-2223/CdSe hetero-nanostructures

One of the innovative technological directions for the high-temperature superconductors has been persued by fabricating the heteroepitaxial multilayer structures such as superconductor-semiconductor heterostructures. In the present investigation, metal/superconductor/semiconductor (Ag/Tl-2223/CdSe) hetero-nanostructures have successfully been fabricated using dc electrodeposition technique and were characterized by X-ray diffraction (XRD), full-width at half-maximum (FWHM) and scanning electron microscopy (SEM) studies. The measurement of junction capacitance as a function of biasing voltage was used for the estimation of junction built-in-potential (V _D) and to study the charge distribution in a heterojunction. The Mott-Schottky plots were measured for each junction in dark and under the photo-irradiation. The effect of laser irradiation on C-V characteristics of hetero-nanostructure has been studied.     

On nonstationary flow through porous media

Summary Saturated-unsaturated flow of an incompressible fluid through a porous medium is considered in the case of time-dependent water levels. This corresponds to coupling the mass conservation law with a continuous constitutive condition between water content and pressure. An existence result for the corresponding weak formulation is proved. Finally we study the limit as the constitutive relation degenerates into a maximal monotone graph.This work was supported by the Deutsche Forschungsgemeinschaft through Sonderforschungsbereich 123 (Heidelberg).On leave from: Istituto di Analisi Numerica del C.N.R., Corso C. Alberto 5 - I 27100 Pavia (Italy).