Giloy Tulsi tablet is an Ayurvedic preparation containing Tinospora cordifolia (Giloy) and Ocimum sanctum (Tulsi) and it is recommended for boosting the body’s immune response. This research work is about the marker-based standardization of this Ayurvedic preparation using high-performance thin-layer chromatography method. Standardization is based on the determination and quantification of the phytoconstituents berberine and ursolic acid present in Giloy Tulsi tablets. Separation was performed on pre-coated silica gel 60 F254 plate as the stationary phase, with chloroform‒acetone‒formic acid (6:3.5:0.5, V/V) as the mobile phase. Identification and quantification were conducted densitometrically at 330 nm. The developed method resulted in good quality peak shape and enabled high-quality resolution of biomarkers. The RF value for berberine (0.46 ± 0.02) and for ursolic acid (0.68 ± 0.02) in both reference standard and formulation were found to be comparable. The method was validated for specificity, linearity, limit of detection, limit of quantification, intra-day and inter-day precisions, accuracy, and robustness. The limit of detection values were 91 and 153 ng/band for berberine and ursolic acid, respectively. The limit of quantification values were 175 and 465 ng/band for berberine and ursolic acid, respectively. Regression analysis of the calibration data revealed a good linear relationship between peak area response and concentration in the range 200‒1000 ng/band for berberine (r2 = 0.995) and 500‒2500 ng/band for ursolic acid (r2 = 0.9968). The accuracy of the method, determined by measurement of recovery at three different levels, was in the range 98‒102% for both markers. These results are indication of reliability, reproducibility, accuracy, and precision of the method.
相似文献The miscibility, crystallization and melting behaviour of poly(trimethylene terephthalate)/thermotropic liquid crystalline polymer (LCP) blends were studied using differential scanning calorimetry. The blends were found to form primarily an immiscible system. The addition of LCP accelerated the overall rate of crystallization and caused a depression in equilibrium melting temperature, especially at low LCP content. Lauritzen–Hoffman analysis showed that the addition of LCP caused a reduction in the fold surface energy and increased the regime II to III transition temperature.
相似文献Himalaya PartySmart capsule is a polyherbal formulation recommended for its liver-protective properties. As the formulation contains extracts of six different herbs, a large number of markers are present in the same. This research work reports the standardization of Himalaya PartySmart capsule using andrographolide and catechin as therapeutic phytoconstituents to assess its quality and efficacy. A specific, sensitive, precise, and accurate high-performance thin-layer chromatography (HPTLC) method has been developed for the quantitative estimation of andrographolide and catechin. Separation was performed on TLC silica gel 60 F254 aluminum plates as the stationary phase using chloroform‒acetone‒formic acid (7:3:0.5, V/V) as the mobile phase with densitometric detection at 259 nm. The developed method was validated as per the recommendations of the International Council for Harmonisation (ICH) Q2(R1) guideline. Each marker phytoconstituent showed a good linear relationship with an average correlation coefficient (r2) = 0.99 in the concentration range studied. The proposed method was found to be specific, precise, and accurate with recovery within the range of 95‒105% and hence can be used for the routine analysis of PartySmart capsule formulation.
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